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Literature summary extracted from

  • Palma-Orozco, G.; Ortiz-Moreno, A.; Dorantes-Alvarez, L.; Sampedro, J.G.; Najera, H.
    Purification and partial biochemical characterization of polyphenol oxidase from mamey (Pouteria sapota) (2011), Phytochemistry, 72, 82-88.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.10.3.1 benzoic acid 134% relative activity at 10 mM Pouteria sapota
1.10.3.1 Citric acid 148% relative activity at 10 mM Pouteria sapota
1.10.3.1 potassium sorbate 158% relative activity at 10 mM Pouteria sapota

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.1 ascorbic acid one of the most effective inhibitors of isozyme PPO 1, complete inhibition at 0.1 mM Pouteria sapota
1.10.3.1 EDTA 5% residual activity at 0.1 mM Pouteria sapota
1.10.3.1 kojic acid 43% residual activity at 1 mM Pouteria sapota
1.10.3.1 NaCl 29% residual activity at 0.1 mM Pouteria sapota
1.10.3.1 Sodium metabisulfite one of the most effective inhibitors of isozyme PPO 1, complete inhibition at 0.1 mM Pouteria sapota
1.10.3.1 succinic acid complete inhibition at 1 mM Pouteria sapota
1.10.3.1 tropolone 68% residual activity at 0.1 mM Pouteria sapota

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.1 1.3
-
 pyrogallol isozyme PPO 1, in 0.2 M sodium phosphate buffer pH 7.0, 25°C Pouteria sapota
1.10.3.1 44
-
 catechol isozyme PPO 1, in 0.2 M sodium phosphate buffer pH 7.0, 25°C Pouteria sapota

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.1 16100
-
 gel filtration Pouteria sapota
1.10.3.1 18000
-
 1 * 18000, SDS-PAGE Pouteria sapota

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.1 Pouteria sapota
-
 mamey
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.1 ammonium sulfate precipitation, and phenyl Sepharose column chromatography Pouteria sapota

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.1 fruit
-
 Pouteria sapota
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.1 1.32
-
 crude extract, at pH 7.0, 25°C Pouteria sapota
1.10.3.1 8.76
-
 isozyme PPO 1 after 6.6fold purification, at pH 7.0, 25°C Pouteria sapota
1.10.3.1 241.5
-
 isozyme PPO 2 after 183fold purification, at pH 7.0, 25°C Pouteria sapota

Storage Stability

EC Number Storage Stability Organism
1.10.3.1 isozyme PPO 2 is highly unstable such as after 24 h of storage PPO 2 activity is not detected Pouteria sapota

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.1 2 catechol + O2 100% activity with 10 mM substrate concentration Pouteria sapota 2 1,2-benzoquinone + 2 H2O
-
 ?
1.10.3.1 4-methylcatechol + O2 208% activity with 10 mM substrate concentration compared to catechol Pouteria sapota 4-methyl-1,2-benzoquinone + H2O
-
 ?
1.10.3.1 additional information the enzyme shows no activity towards caffeic acid, DL-Dopa, ferulic acid and phenol Pouteria sapota ?
-
 ?
1.10.3.1 pyrogallol + O2 746% activity with 10 mM substrate concentration compared to catechol Pouteria sapota ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.1 monomer 1 * 18000, SDS-PAGE Pouteria sapota

Synonyms

EC Number Synonyms Comment Organism
1.10.3.1 polyphenol oxidase
-
 Pouteria sapota
1.10.3.1 PPO 1 isozyme Pouteria sapota
1.10.3.1 PPO 2 isozyme Pouteria sapota

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.1 35 45 optimum temperature for isozyme PPO 1 Pouteria sapota

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.1 20 70 at 20°C the residual activity (26%) is maintained, at higher temperatures the activity decreases (50-60°C) and from 65 to 70°C there is no activity Pouteria sapota

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.1 35
-
 PPO is maximum thermally stable up to 35°C Pouteria sapota

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.1 7
-
 optimum pH for isozyme PPO 1 Pouteria sapota

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.1 6 7.5 at pH 6.0, isozyme PPO 1 activity increases drastically, reaching maximum activity at pH 7.0 and then again radically decreases at pH 7.5 Pouteria sapota