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Literature summary extracted from

  • Lopez, A.B.; Yang, Y.; Thannhauser, T.W.; Li, L.
    Phytoene desaturase is present in a large protein complex in the plastid membrane (2008), Physiol. Plant., 133, 190-198.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.5.5 heterologous expression in a strain of Escherichia coli containing a carotenoid-producing plasmid Brassica oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.5.5 chloroplast the plastid membrane enzyme form is present in a large protein complex of approximately 350000 Da, the enzyme from stroma is in an approximately 660000 Da complex Brassica oleracea 9507
-
1.3.5.5 chromoplast BoPDS is present at similar levels in chromoplasts and leucoplasts Brassica oleracea 9509
-
1.3.5.5 leucoplast BoPDS is present at similar levels in chromoplasts and leucoplasts Brassica oleracea 9516
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.5.5 55000
-
 x * 55000, SDS-PAGE, the plastid membrane enzyme form is present in a large protein complex of approximately 350000 Da, the stroma version is in an approximately 660000 Da complex Brassica oleracea
1.3.5.5 350000
-
 the plastid membrane enzyme form is present in a large protein complex of approximately 350000 Da, the stroma version is in an approximately 660000 Da complex Brassica oleracea
1.3.5.5 660000
-
 the plastid membrane enzyme form is present in a large protein complex of approximately 350000 Da, the stroma version is in an approximately 660000 Da complex Brassica oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.5.5 15-cis-phytoene + acceptor Brassica oleracea phytoene desaturase is an early enzyme of the carotenoid biosynthetic pathway 9,15,9'-tricis-zeta-carotene + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.5.5 Brassica oleracea B2X8P6 var. botrytis, white cultivar Stovepipe
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.3.5.5 additional information the enzyme form from stroma appears to be slightly larger in size than the membrane version, which may be because of different posttranslational modifications of BoPDS in these two locations Brassica oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.5.5 flower BoPDS has the highest level of expression in flowers with an approximate 4.5fold increase of BoPDS transcript level compared with leaf tissue Brassica oleracea
-
1.3.5.5 leaf BoPDS has the highest level of expression in flowers with an approximate 4.5fold increase of BoPDS transcript level compared with leaf tissue Brassica oleracea
-
1.3.5.5 root siliques and roots show the lowest levels of expression Brassica oleracea
-
1.3.5.5 seed
-
 Brassica oleracea
-
1.3.5.5 silique siliques and roots show the lowest levels of expression Brassica oleracea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.5.5 15-cis-phytoene + acceptor
-
 Brassica oleracea 9,15,9'-tricis-zeta-carotene + reduced acceptor
-
 ?
1.3.5.5 15-cis-phytoene + acceptor phytoene desaturase is an early enzyme of the carotenoid biosynthetic pathway Brassica oleracea 9,15,9'-tricis-zeta-carotene + reduced acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.5.5 ? x * 55000, SDS-PAGE, the plastid membrane enzyme form is present in a large protein complex of approximately 350000 Da, the stroma version is in an approximately 660000 Da complex Brassica oleracea

Synonyms

EC Number Synonyms Comment Organism
1.3.5.5 BoPDS
-
 Brassica oleracea