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Literature summary extracted from

  • Kempenaers, M.; Roovers, M.; Oudjama, Y.; Tkaczuk, K.L.; Bujnicki, J.M.; Droogmans, L.
    New archaeal methyltransferases forming 1-methyladenosine or 1-methyladenosine and 1-methylguanosine at position 9 of tRNA (2010), Nucleic Acids Res., 38, 6533-6543.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.218 expression in Escherichia coli Thermococcus kodakarensis
2.1.1.218 expression in Escherichia coli Sulfolobus acidocaldarius
2.1.1.221 expression in Escherichia coli Thermococcus kodakarensis
2.1.1.221 expression in Escherichia coli Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.218 S-adenosyl-L-methionine + adenine9 in tRNA Sulfolobus acidocaldarius
-
 S-adenosyl-L-homocysteine + N1-methyladenine9 in tRNA
-
 ?
2.1.1.218 S-adenosyl-L-methionine + adenine9 in tRNA Thermococcus kodakarensis the bifunctional enzyme catalyzes both methylation of guanine9 and methylation of adenine9 in tRNA S-adenosyl-L-homocysteine + N1-methyladenine9 in tRNA
-
 ?
2.1.1.221 S-adenosyl-L-methionine + guanine9 in tRNA Saccharomyces cerevisiae
-
 S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNA
-
 ?
2.1.1.221 S-adenosyl-L-methionine + guanine9 in tRNA Thermococcus kodakarensis the bifunctional enzyme catalyzes both methylation of guanine9 and methylation of adenine9 in tRNA S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.218 Sulfolobus acidocaldarius Q4J894
-
-
2.1.1.218 Thermococcus kodakarensis Q5JD38
-
-
2.1.1.221 Saccharomyces cerevisiae Q12400
-
-
2.1.1.221 Thermococcus kodakarensis Q5JD38
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.218
-
 Thermococcus kodakarensis
2.1.1.218
-
 Sulfolobus acidocaldarius
2.1.1.221
-
 Thermococcus kodakarensis
2.1.1.221
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.218 S-adenosyl-L-methionine + adenine9 in tRNA
-
 Sulfolobus acidocaldarius S-adenosyl-L-homocysteine + N1-methyladenine9 in tRNA
-
 ?
2.1.1.218 S-adenosyl-L-methionine + adenine9 in tRNA the bifunctional enzyme catalyzes both methylation of guanine9 and methylation of adenine9 in tRNA Thermococcus kodakarensis S-adenosyl-L-homocysteine + N1-methyladenine9 in tRNA
-
 ?
2.1.1.218 S-adenosyl-L-methionine + adenine9 in tRNA formation of N1-methyladenosine9 in tRNAAla from Thermococcus kodakaraensis that contains an adenine at position 9. The enzyme forms approximately the same amount of m1A and m1G when the tRNA of the yeast strain Y16243 is used as substrate. Given that occurrence of A9 and G9 in this tRNA population is almost equal (about 50% each) this result indicates that the enzyme TK0422p does not show any preference for one of these two nucleosides. The ratio m1A/m1G formed from Escherichia coli tRNA is higher than that with tRNA from the yeast Y16243 strain. This is consistent with the fact that there are about two times more tRNAs with A9 than with G9 in Escherichia coli. The enzyme is active in a pH range 5.5-9.75. The intensity of m1A and m1G spots varies greatly as a function of the pH. At pH 5.5, m1A MTase activity of TK0422p is predominant over m1G. At pH 7 or higher, both m1A and m1G are detected, m1G intensity growing with increasing pH Thermococcus kodakarensis S-adenosyl-L-homocysteine + N1-methyladenine9 in tRNA
-
 ?
2.1.1.218 S-adenosyl-L-methionine + adenine9 in tRNA the enzyme is specific for adenine9 in tRNA, does not catalyse the methylation of guanine9 in tRNA from Saccharomyces cerevisiae. No m1A is formed in a mutant of the tRNAMeti where position 9 is occupied by a guanosine (tRNAMeti A9G) Sulfolobus acidocaldarius S-adenosyl-L-homocysteine + N1-methyladenine9 in tRNA
-
 ?
2.1.1.221 S-adenosyl-L-methionine + guanine9 in tRNA
-
 Saccharomyces cerevisiae S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNA
-
 ?
2.1.1.221 S-adenosyl-L-methionine + guanine9 in tRNA the bifunctional enzyme catalyzes both methylation of guanine9 and methylation of adenine9 in tRNA Thermococcus kodakarensis S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNA
-
 ?
2.1.1.221 S-adenosyl-L-methionine + guanine9 in tRNA formation of N1-methylguanine9 in tRNA(Asp) from Thermococcus kodakaraensis that contains a guanosine at position 9. The enzyme forms approximately the same amount of m1A and m1G when the tRNA of the yeast strain Y16243 is used as substrate. Given that occurrence of A9 and G9 in this tRNA population is almost equal (about 50% each) this result indicates that the enzyme TK0422p does not show any preference for one of these two nucleosides. The ratio m1A/m1G formed from Escherichia coli tRNA is higher than that with tRNA from the yeast Y16243 strain. This is consistent with the fact that there are about two times more tRNAs with A9 than with G9 in Escherichia coli. The enzyme is active in a pH range 5.5-9.75. The intensity of m1A and m1G spots varies greatly as a function of the pH. At pH 5.5, m1A MTase activity of TK0422p is predominant over m1G. At pH 7 or higher, both m1A and m1G are detected, m1G intensity growing with increasing pH Thermococcus kodakarensis S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNA
-
 ?
2.1.1.221 S-adenosyl-L-methionine + guanine9 in tRNA the enzyme is specific for guanine9 in tRNA, does not catalyse methylation of adenine9 in tRNA Saccharomyces cerevisiae S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.218 TK0422p
-
 Thermococcus kodakarensis
2.1.1.218 Trm10p (ambiguous)
-
 Thermococcus kodakarensis
2.1.1.218 tRNA m1A9 MTase
-
 Sulfolobus acidocaldarius
2.1.1.218 tRNA m1A9-methyltransferase
-
 Sulfolobus acidocaldarius
2.1.1.218 tRNA(m1G9/m1A9)-methyltransferase
-
 Thermococcus kodakarensis
2.1.1.218 tRNA(m1G9/m1A9)MTase
-
 Thermococcus kodakarensis
2.1.1.221 TK0422p
-
 Thermococcus kodakarensis
2.1.1.221 Trm10p (ambiguous)
-
 Thermococcus kodakarensis
2.1.1.221 tRNA(m1G9/m1A9)-methyltransferase
-
 Thermococcus kodakarensis
2.1.1.221 tRNA(m1G9/m1A9)MTase
-
 Thermococcus kodakarensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.218 50
-
 assay at Thermococcus kodakarensis
2.1.1.218 50
-
 assay at Sulfolobus acidocaldarius
2.1.1.221 30
-
 assay at Saccharomyces cerevisiae
2.1.1.221 50
-
 assay at Thermococcus kodakarensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.218 8
-
 assay at Thermococcus kodakarensis
2.1.1.218 8
-
 assay at Sulfolobus acidocaldarius
2.1.1.221 8
-
 assay at Thermococcus kodakarensis
2.1.1.221 8
-
 assay at Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.218 5.5 9.75 the bifunctional enzyme is active in a pH range 5.5-9.75. The intensity of m1A and m1G spots varies greatly as a function of the pH. At pH 5.5, m1A MTase activity of TK0422p is predominant over m1G. At pH 7 or higher, both m1A and m1G are detected, m1G intensity growing with increasing pH Thermococcus kodakarensis
2.1.1.221 5.5 9.75 the bifunctional enzyme is active in a pH range 5.5-9.75. The intensity of m1A and m1G spots varies greatly as a function of the pH. At pH 5.5, m1A MTase activity of TK0422p is predominant over m1G. At pH 7 or higher, both m1A and m1G are detected, m1G intensity growing with increasing pH Thermococcus kodakarensis