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Literature summary extracted from

  • Taylor, R.C.; Brown, A.K.; Singh, A.; Bhatt, A.; Besra, G.S.
    Characterization of a beta-hydroxybutyryl-CoA dehydrogenase from Mycobacterium tuberculosis (2010), Microbiology, 156, 1975-1982.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.35 overexpression in Escherichia coli Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.35 Co2+ 1 mM, about 50% inhibition Mycobacterium tuberculosis
1.1.1.35 EDTA about 35% inhibition at 1 mM, about 25% inhibition at 10 mM Mycobacterium tuberculosis
1.1.1.35 K+ about 30% inhibition at 1 mM, about 20% inhibition at 10 mM Mycobacterium tuberculosis
1.1.1.35 Ni2+ 1 mM, about 60% inhibition Mycobacterium tuberculosis
1.1.1.35 Zn2+ 1 mM, about 60% inhibition Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.35 29.5
-
 NAD+ pH 9.5, 30°C Mycobacterium tuberculosis
1.1.1.35 43.5
-
 (S)-3-hydroxybutyryl-CoA pH 9.5, 30°C Mycobacterium tuberculosis
1.1.1.35 50
-
 NADH pH 7.0, 30°C Mycobacterium tuberculosis
1.1.1.35 65.6
-
 3-acetoacetyl-CoA pH 7.0, 30°C Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.35 Ca2+ enhances activity at 1 mM or 10 mM Mycobacterium tuberculosis
1.1.1.35 Mg2+ enhances activity at 1 mM or 10 mM Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.35 (S)-3-hydroxybutyryl-CoA + NAD+ Mycobacterium tuberculosis the enzyme is involved in betaq-oxidation cycle 3-acetoacetyl-CoA + NADH + H+
-
 r
1.1.1.35 (S)-3-hydroxybutyryl-CoA + NAD+ Mycobacterium tuberculosis ATCC 25618 the enzyme is involved in betaq-oxidation cycle 3-acetoacetyl-CoA + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.35 Mycobacterium tuberculosis P9WNP7
-
-
1.1.1.35 Mycobacterium tuberculosis ATCC 25618 P9WNP7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.35
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.35 (S)-3-hydroxybutyryl-CoA + NAD+ the enzyme is involved in betaq-oxidation cycle Mycobacterium tuberculosis 3-acetoacetyl-CoA + NADH + H+
-
 r
1.1.1.35 (S)-3-hydroxybutyryl-CoA + NAD+ no activity woth NADP+ Mycobacterium tuberculosis 3-acetoacetyl-CoA + NADH + H+
-
 r
1.1.1.35 (S)-3-hydroxybutyryl-CoA + NAD+ the enzyme is involved in betaq-oxidation cycle Mycobacterium tuberculosis ATCC 25618 3-acetoacetyl-CoA + NADH + H+
-
 r
1.1.1.35 (S)-3-hydroxybutyryl-CoA + NAD+ no activity woth NADP+ Mycobacterium tuberculosis ATCC 25618 3-acetoacetyl-CoA + NADH + H+
-
 r
1.1.1.35 3-acetoacetyl-CoA + NADH + H+
-
 Mycobacterium tuberculosis (S)-3-hydroxybutyryl-CoA + NAD+
-
 r
1.1.1.35 3-acetoacetyl-CoA + NADH + H+
-
 Mycobacterium tuberculosis ATCC 25618 (S)-3-hydroxybutyryl-CoA + NAD+
-
 r
1.1.1.35 3-acetoacetyl-CoA + NADPH + H+ no dehydration of beta-hydroxybutyryl-CoA to acetoacetyl-CoA with NADP+ as cofactor Mycobacterium tuberculosis (S)-3-hydroxybutyryl-CoA + NAD+
-
 ir
1.1.1.35 3-acetoacetyl-CoA + NADPH + H+ no dehydration of beta-hydroxybutyryl-CoA to acetoacetyl-CoA with NADP+ as cofactor Mycobacterium tuberculosis ATCC 25618 (S)-3-hydroxybutyryl-CoA + NAD+
-
 ir

Synonyms

EC Number Synonyms Comment Organism
1.1.1.35 FadB2
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.35 37
-
 NAD+-dependent dehydration of beta-hydroxybutyryl-CoA to acetoacetyl-CoA Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.35 0.004
-
 NAD+ pH 9.5, 30°C Mycobacterium tuberculosis
1.1.1.35 0.011
-
 3-acetoacetyl-CoA pH 7.0, 30°C Mycobacterium tuberculosis
1.1.1.35 0.012
-
 (S)-3-hydroxybutyryl-CoA pH 9.5, 30°C Mycobacterium tuberculosis
1.1.1.35 0.189
-
 NADH pH 7.0, 30°C Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.35 5.5 6.5 conversion of acetoacetyl-CoA to beta-hydroxybutyryl-CoA decreases above pH 6.5 Mycobacterium tuberculosis
1.1.1.35 10
-
 NAD+-dependent dehydration of beta-hydroxybutyryl-CoA to acetoacetyl-CoA Mycobacterium tuberculosis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.35 9 11 pH 9.0: about 45% of maximal activity, pH 11.0: about 55% of maximal activity Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.35 NAD+ no dehydration of beta-hydroxybutyryl-CoA to acetoacetyl-CoA with NADP+ as cofactor Mycobacterium tuberculosis
1.1.1.35 NADH conversion of acetoacetyl-CoA to beta-hydroxybutyryl-CoA Mycobacterium tuberculosis
1.1.1.35 NADPH conversion of acetoacetyl-CoA to beta-hydroxybutyryl-CoA Mycobacterium tuberculosis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.35 Mycobacterium tuberculosis calculated, native protein
-
 5.2
1.1.1.35 Mycobacterium tuberculosis calculated, recombinant protein
-
 6.1

General Information

EC Number General Information Comment Organism
1.1.1.35 malfunction a null mutant of fadB2 shows no significant differences from the wild-type strain with regard to lipid composition, utilization of different fatty acid carbon sources and tolerance to various stresses Mycobacterium tuberculosis
1.1.1.35 metabolism the enzyme is involved in beta-oxidation cycle Mycobacterium tuberculosis
1.1.1.157 physiological function null mutant shows no significant differences from the wild-type strain with regard to lipid composition, utilization of different fatty acid carbon sources and tolerance to various stresses Mycobacterium tuberculosis