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Literature summary extracted from

  • Salaheddin, C.; Takakura, Y.; Tsunashima, M.; Stranzinger, B.; Spadiut, O.; Yamabhai, M.; Peterbauer, C.K.; Haltrich, D.
    Characterisation of recombinant pyranose oxidase from the cultivated mycorrhizal basidiomycete Lyophyllum shimeji (hon-shimeji) (2010), Microb. Cell Fact., 9, 57.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.1.3.10 biotechnology P2Ox is a biocatalyst with high potential for biotransformations of carbohydrates and in synthetic carbohydrate chemistry. P2Ox is useful as bioelement in biofuel cells, replacing glucose oxidase Lyophyllum shimeji

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.10 expressed in Escherichia coli Rosetta 2(DE3) cells Lyophyllum shimeji

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.10 0.033
-
1,4-benzoquinone using D-glucose as cosubstrate, at 30°C, pH 6.5 Lyophyllum shimeji
1.1.3.10 0.187
-
2,6-dichloroindophenol using D-glucose as cosubstrate, at 30°C, pH 6.5 Lyophyllum shimeji
1.1.3.10 0.187
-
ferrocenium ion using D-glucose as cosubstrate, at 30°C, pH 6.5 Lyophyllum shimeji
1.1.3.10 0.314
-
D-glucose at 30°C, pH 6.5 Lyophyllum shimeji
1.1.3.10 3.84
-
D-galactose at 30°C, pH 6.5 Lyophyllum shimeji
1.1.3.10 6.3
-
D-xylose at 30°C, pH 6.5 Lyophyllum shimeji
1.1.3.10 14.7
-
L-sorbose at 30°C, pH 6.5 Lyophyllum shimeji
1.1.3.10 72.8
-
melibiose at 30°C, pH 6.5 Lyophyllum shimeji

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.10 68000
-
gel filtration Lyophyllum shimeji
1.1.3.10 68490
-
calculated from amino acid sequence Lyophyllum shimeji

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.10 Lyophyllum shimeji Q75ZP8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.10 anion exchange chromatography and preparative native PAGE Lyophyllum shimeji

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.10 D-galactose + O2
-
Lyophyllum shimeji 2-dehydro-D-galactose + H2O2
-
?
1.1.3.10 D-glucose + 1,4-benzoquinone
-
Lyophyllum shimeji 2-dehydro-D-glucose + 1,4-hydroquinone
-
?
1.1.3.10 D-glucose + 2,6-dichloroindophenol
-
Lyophyllum shimeji 2-dehydro-D-glucose + ?
-
?
1.1.3.10 D-glucose + ferrocenium ion
-
Lyophyllum shimeji 2-dehydro-D-glucose + ?
-
?
1.1.3.10 D-glucose + O2
-
Lyophyllum shimeji 2-dehydro-D-glucose + H2O2
-
?
1.1.3.10 D-xylose + O2
-
Lyophyllum shimeji D-xylosone + H2O2
-
?
1.1.3.10 L-sorbose + O2
-
Lyophyllum shimeji 5-dehydro-D-fructose + H2O2
-
?
1.1.3.10 melibiose + O2
-
Lyophyllum shimeji ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.10 P2Ox
-
Lyophyllum shimeji
1.1.3.10 pyranose 2-Oxidase
-
Lyophyllum shimeji
1.1.3.10 pyranose:oxygen 2-oxidoreductase
-
Lyophyllum shimeji

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.10 40 55 the melting temperature is 54.9°C, half-life time of activity is 0.12 min at 50°C and pH 6.5, half-life time of activity is 60 h at 40°C and pH 6.5/8.0 and 43 min at 40°C and pH 4.0 Lyophyllum shimeji

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.10 0.839
-
melibiose at 30°C, pH 6.5 Lyophyllum shimeji
1.1.3.10 1.02
-
D-galactose at 30°C, pH 6.5 Lyophyllum shimeji
1.1.3.10 5.48
-
D-xylose at 30°C, pH 6.5 Lyophyllum shimeji
1.1.3.10 6.92
-
D-glucose at 30°C, pH 6.5 Lyophyllum shimeji
1.1.3.10 7.7
-
L-sorbose at 30°C, pH 6.5 Lyophyllum shimeji
1.1.3.10 39.9
-
ferrocenium ion using D-glucose as cosubstrate, at 30°C, pH 6.5 Lyophyllum shimeji
1.1.3.10 67.3
-
2,6-dichloroindophenol using D-glucose as cosubstrate, at 30°C, pH 6.5 Lyophyllum shimeji
1.1.3.10 92.3
-
1,4-benzoquinone using D-glucose as cosubstrate, at 30°C, pH 6.5 Lyophyllum shimeji

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.10 FAD dependent on Lyophyllum shimeji

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.3.10 0.0115
-
melibiose at 30°C, pH 6.5 Lyophyllum shimeji
1.1.3.10 0.265
-
D-galactose at 30°C, pH 6.5 Lyophyllum shimeji
1.1.3.10 0.524
-
L-sorbose at 30°C, pH 6.5 Lyophyllum shimeji
1.1.3.10 0.867
-
D-xylose at 30°C, pH 6.5 Lyophyllum shimeji
1.1.3.10 22.1
-
D-glucose at 30°C, pH 6.5 Lyophyllum shimeji
1.1.3.10 213
-
ferrocenium ion using D-glucose as cosubstrate, at 30°C, pH 6.5 Lyophyllum shimeji
1.1.3.10 361
-
2,6-dichloroindophenol using D-glucose as cosubstrate, at 30°C, pH 6.5 Lyophyllum shimeji
1.1.3.10 2760
-
1,4-benzoquinone using D-glucose as cosubstrate, at 30°C, pH 6.5 Lyophyllum shimeji