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Literature summary extracted from

  • Even, S.; Garrigues, C.; Loubiere, P.; Lindley, N.D.; Cocaign-Bousquet, M.
    Pyruvate metabolism in Lactococcus lactis is dependent upon glyceraldehyde-3-phosphate dehydrogenase activity (1999), Metab. Eng., 1, 198-205.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.12 iodoacetate
-
 Lactococcus lactis
1.2.1.12 additional information no inhibition by D-glucose 6-phosphate, D-fructose 6-phosphate, D-fructose 1,6-bisphosphate, dihydroxyacetone phosphate, phosphoenolpyruvate, pyruvate Lactococcus lactis
1.2.1.12 NADH competitive Lactococcus lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.12 0.2
-
 NAD+ 30°C, pH 7.2 Lactococcus lactis
1.2.1.12 0.55
-
 D-glyceraldehyde 3-phosphate 30°C, pH 7.2 Lactococcus lactis

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.12 Lactococcus lactis
-
-
-
1.2.1.12 Lactococcus lactis NCDO 2118
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.12
-
 Lactococcus lactis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Storage Stability

EC Number Storage Stability Organism
1.2.1.12 -20°C Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Lactococcus lactis 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Lactococcus lactis NCDO 2118 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.12 70
-
 NAD+ 30°C, pH 7.2 Lactococcus lactis

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.12 NAD+
-
 Lactococcus lactis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.1.12 0.02
-
 NADH 30°C, pH 7.2, competitive inhibitor, no effect on the maximum velocity Lactococcus lactis
1.2.1.12 0.145
-
 iodoacetate 30°C, pH 7.2 Lactococcus lactis