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Literature summary extracted from

  • Keighron, J.D.; Keating, C.D.
    Enzyme:nanoparticle bioconjugates with two sequential enzymes: stoichiometry and activity of malate dehydrogenase and citrate synthase on Au nanoparticles (2010), Langmuir, 26, 18992-19000.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.37 additional information adsorption of the enzyme in 30 nm diameter Au nanoparticles for synthesis of bioconjugates, MDH:Au stoichiometry and kinetic parameters determined for MDH:Au, citrate synthase CS:Au, and three types of dual-activity MDH/CS:Au bioconjugates, method development, overview. The number of enzyme molecules adsorbed per particle is dependent upon the enzyme concentration in solution, with multilayers forming at high enzyme:Au solution ratios. The specific activity of adsorbed enzyme increases with increasing number adsorbed per particle for CS:Au, but is less sensitive to stoichiometry for MDH:Au Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.37 0.02
-
 oxaloacetate pH 8.1, 22°C Sus scrofa
1.1.1.37 0.3
-
 (S)-malate pH 8.1, 22°C Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.37 (S)-malate + NAD+ Sus scrofa
-
 oxaloacetate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.37 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.37 commercial preparation
-
 Sus scrofa
-
1.1.1.37 heart
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.37 182
-
 pH 8.1, 22°C, specific activity of free and conjugated enzymes prepared at 300:1 solution enzyme:Au ratio Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.37 (S)-malate + NAD+
-
 Sus scrofa oxaloacetate + NADH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.37 malate dehydrogenase
-
 Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.37 22
-
 assay at room temperature, oxidation and reduction Sus scrofa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.37 18
-
 (S)-malate pH 8.1, 22°C Sus scrofa
1.1.1.37 377
-
 oxaloacetate pH 8.1, 22°C Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.37 8.1
-
 assay at, oxidation and reduction Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.37 NAD+
-
 Sus scrofa
1.1.1.37 NADH
-
 Sus scrofa