Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kostan, J.; Sjoeblom, B.; Maixner, F.; Mlynek, G.; Furtmueller, P.G.; Obinger, C.; Wagner, M.; Daims, H.; Djinovic-Carugo, K.
    Structural and functional characterisation of the chlorite dismutase from the nitrite-oxidizing bacterium Candidatus Nitrospira defluvii: identification of a catalytically important amino acid residue (2010), J. Struct. Biol., 172, 331-342.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.13.11.49 environmental protection bacteria with Cld play significant roles in the bioremediation of industrially contaminated sites and also in wastewater treatment Nitrospira defluvii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.49 phylogenetic analysis, expression of N-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Nitrospira defluvii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.11.49 purified recombinant His-tagged wild-type and mutant enzymes with bound cyanide, 22°C, sitting drop vapour diffusion method, X-ray diffraction structure determination and analysis at 1.85-2.70 A resolution Nitrospira defluvii

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.49 R173A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme, structure determination and comparison to the wild-type enzyme Nitrospira defluvii
1.13.11.49 R173K site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme, structure determination and comparison to the wild-type enzyme Nitrospira defluvii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.49 additional information
-
 additional information steady-state, pre-steady-state, and transient-state kinetics, overview Nitrospira defluvii
1.13.11.49 0.057
-
 Chlorite pH 7.0, 30°C, recombinant wild-type enzyme Nitrospira defluvii
1.13.11.49 0.107
-
 Chlorite pH 7.0, 30°C, recombinant mutant R137A Nitrospira defluvii
1.13.11.49 0.818
-
 Chlorite pH 7.0, 30°C, recombinant mutant R137K Nitrospira defluvii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.49 CN- binding kinetics of wild-type and mutant enzymes, overview Nitrospira defluvii
1.13.11.49 Fe2+ heme protein Nitrospira defluvii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.49 chloride + O2 Nitrospira defluvii chlorite dismutase is a unique heme enzyme which transforms chlorite to chloride and molecular oxygen chlorite
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.49 Nitrospira defluvii B3U4H7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.49 recombinant N-terminally His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Nitrospira defluvii

Reaction

EC Number Reaction Comment Organism Reaction ID
1.13.11.49 chloride + O2 = chlorite reaction mechanism and active site structure, Arg173 plays a key role in (i) controlling of ligand and substrate access and binding and (ii) in chlorite dismutation reaction, overview Nitrospira defluvii
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.49 chloride + O2 chlorite dismutase is a unique heme enzyme which transforms chlorite to chloride and molecular oxygen Nitrospira defluvii chlorite
-
 r
1.13.11.49 chloride + O2 Arg173 plays a key role in (i) controlling of ligand and substrate access and binding and (ii) in chlorite dismutation reaction, mechanism, overview. The flexible residue modulates the electrostatic potential and size of the active site entrance and might be involved in keeping transiently formed hypochlorite in place for final molecular oxygen and chloride formation Nitrospira defluvii chlorite
-
 r

Subunits

EC Number Subunits Comment Organism
1.13.11.49 homopentamer each monomer of NdCld is characterized by two topologically equivalent four-stranded antiparallel beta-sheets forming a beta-barrel, flanked on both sides by six alpha-helices, overview Nitrospira defluvii

Synonyms

EC Number Synonyms Comment Organism
1.13.11.49 chlorite dismutase
-
 Nitrospira defluvii
1.13.11.49 CLD
-
 Nitrospira defluvii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.49 25 30 assay at Nitrospira defluvii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.49 7.1
-
 Chlorite pH 7.0, 30°C, recombinant mutant R137A Nitrospira defluvii
1.13.11.49 21
-
 Chlorite pH 7.0, 30°C, recombinant mutant R137K Nitrospira defluvii
1.13.11.49 35
-
 Chlorite pH 7.0, 30°C, recombinant wild-type enzyme Nitrospira defluvii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.49 7
-
 assay at Nitrospira defluvii

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.11.49 heme
-
 Nitrospira defluvii

General Information

EC Number General Information Comment Organism
1.13.11.49 evolution active site conservation and evolution of Cld, overview Nitrospira defluvii
1.13.11.49 additional information Candidatus Nitrospira defluvii is a key nitrifier in biological wastewater treatment, molecular mechanism of chlorite detoxification, overview. The residue corresponding to Arg173 is conserved in all known active forms of Cld and propose it as a marker for Cld activity in yet uncharacterized Cld-like proteins Nitrospira defluvii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.11.49 25000
-
 Chlorite pH 7.0, 30°C, recombinant mutant R137K Nitrospira defluvii
1.13.11.49 66000
-
 Chlorite pH 7.0, 30°C, recombinant mutant R137A Nitrospira defluvii
1.13.11.49 600000
-
 Chlorite pH 7.0, 30°C, recombinant wild-type enzyme Nitrospira defluvii