EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.4.5 | expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.5.4.5 | hanging drop vapor diffusion method | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.4.5 | 1.004 | - |
cytidine | in 50 mM Tris-HCl buffer pH 7.5 at 25°C | Mycobacterium tuberculosis | |
3.5.4.5 | 1.059 | - |
2'-deoxycytidine | in 50 mM Tris-HCl buffer pH 7.5 at 25°C | Mycobacterium tuberculosis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.4.5 | Zn2+ | CDA is a zinc-dependent metalloenzyme and contains one mol of Zn2+ per mol of enzyme subunit | Mycobacterium tuberculosis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.4.5 | 13938 | - |
4 * 13938, electrospray ionization mass spectrometry | Mycobacterium tuberculosis |
3.5.4.5 | 14000 | - |
4 * 14000, SDS-PAGE | Mycobacterium tuberculosis |
3.5.4.5 | 14072 | - |
4 * 14072, calculated from amino acid sequence | Mycobacterium tuberculosis |
3.5.4.5 | 52990 | - |
gel filtration | Mycobacterium tuberculosis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.4.5 | Mycobacterium tuberculosis | P9WPH3 | - |
- |
3.5.4.5 | Mycobacterium tuberculosis H37Rv | P9WPH3 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.4.5 | Q Sepharose column chromatography, Sephacryl S-200 gel filtration, and butyl-Sepharose column chromatography | Mycobacterium tuberculosis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.4.5 | 1.68 | - |
crude extract, pH 7.5, at 25°C, using 2'-deoxycytidine as substrate | Mycobacterium tuberculosis |
3.5.4.5 | 2.35 | - |
crude extract, pH 7.5, at 25°C, using cytidine as substrate | Mycobacterium tuberculosis |
3.5.4.5 | 3.75 | - |
after 2.2fold purification, pH 7.5, at 25°C, using 2'-deoxycytidine as substrate | Mycobacterium tuberculosis |
3.5.4.5 | 5.44 | - |
after 2.3fold purification, pH 7.5, at 25°C, using cytidine as substrate | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.4.5 | 2'-deoxycytidine + H2O | - |
Mycobacterium tuberculosis | 2'-deoxyuridine + NH3 | - |
? | |
3.5.4.5 | 2'-deoxycytidine + H2O | - |
Mycobacterium tuberculosis H37Rv | 2'-deoxyuridine + NH3 | - |
? | |
3.5.4.5 | cytidine + H2O | - |
Mycobacterium tuberculosis | uridine + NH3 | - |
? | |
3.5.4.5 | cytidine + H2O | - |
Mycobacterium tuberculosis H37Rv | uridine + NH3 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.4.5 | homotetramer | 4 * 13938, electrospray ionization mass spectrometry | Mycobacterium tuberculosis |
3.5.4.5 | homotetramer | 4 * 14000, SDS-PAGE | Mycobacterium tuberculosis |
3.5.4.5 | homotetramer | 4 * 14072, calculated from amino acid sequence | Mycobacterium tuberculosis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.4.5 | CDA | - |
Mycobacterium tuberculosis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.4.5 | 3.5 | - |
2'-deoxycytidine | in 50 mM Tris-HCl buffer pH 7.5 at 25°C | Mycobacterium tuberculosis | |
3.5.4.5 | 4.8 | - |
cytidine | in 50 mM Tris-HCl buffer pH 7.5 at 25°C | Mycobacterium tuberculosis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.5.4.5 | metabolism | CDA is a pyrimidine salvage pathway enzyme that recycles cytidine and 20-deoxycytidine for uridine and 2-deoxyuridine synthesis, respectively | Mycobacterium tuberculosis |