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Literature summary extracted from

  • Sanchez-Quitian, Z.A.; Schneider, C.Z.; Ducati, R.G.; de Azevedo, W.F.; Bloch, C.; Basso, L.A.; Santos, D.S.
    Structural and functional analyses of Mycobacterium tuberculosis Rv3315c-encoded metal-dependent homotetrameric cytidine deaminase (2010), J. Struct. Biol., 169, 413-423.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.5 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.4.5 hanging drop vapor diffusion method Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.5 1.004
-
cytidine in 50 mM Tris-HCl buffer pH 7.5 at 25°C Mycobacterium tuberculosis
3.5.4.5 1.059
-
2'-deoxycytidine in 50 mM Tris-HCl buffer pH 7.5 at 25°C Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.4.5 Zn2+ CDA is a zinc-dependent metalloenzyme and contains one mol of Zn2+ per mol of enzyme subunit Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.5 13938
-
4 * 13938, electrospray ionization mass spectrometry Mycobacterium tuberculosis
3.5.4.5 14000
-
4 * 14000, SDS-PAGE Mycobacterium tuberculosis
3.5.4.5 14072
-
4 * 14072, calculated from amino acid sequence Mycobacterium tuberculosis
3.5.4.5 52990
-
gel filtration Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.5 Mycobacterium tuberculosis P9WPH3
-
-
3.5.4.5 Mycobacterium tuberculosis H37Rv P9WPH3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.5 Q Sepharose column chromatography, Sephacryl S-200 gel filtration, and butyl-Sepharose column chromatography Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.5 1.68
-
crude extract, pH 7.5, at 25°C, using 2'-deoxycytidine as substrate Mycobacterium tuberculosis
3.5.4.5 2.35
-
crude extract, pH 7.5, at 25°C, using cytidine as substrate Mycobacterium tuberculosis
3.5.4.5 3.75
-
after 2.2fold purification, pH 7.5, at 25°C, using 2'-deoxycytidine as substrate Mycobacterium tuberculosis
3.5.4.5 5.44
-
after 2.3fold purification, pH 7.5, at 25°C, using cytidine as substrate Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.5 2'-deoxycytidine + H2O
-
Mycobacterium tuberculosis 2'-deoxyuridine + NH3
-
?
3.5.4.5 2'-deoxycytidine + H2O
-
Mycobacterium tuberculosis H37Rv 2'-deoxyuridine + NH3
-
?
3.5.4.5 cytidine + H2O
-
Mycobacterium tuberculosis uridine + NH3
-
?
3.5.4.5 cytidine + H2O
-
Mycobacterium tuberculosis H37Rv uridine + NH3
-
?

Subunits

EC Number Subunits Comment Organism
3.5.4.5 homotetramer 4 * 13938, electrospray ionization mass spectrometry Mycobacterium tuberculosis
3.5.4.5 homotetramer 4 * 14000, SDS-PAGE Mycobacterium tuberculosis
3.5.4.5 homotetramer 4 * 14072, calculated from amino acid sequence Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
3.5.4.5 CDA
-
Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.4.5 3.5
-
2'-deoxycytidine in 50 mM Tris-HCl buffer pH 7.5 at 25°C Mycobacterium tuberculosis
3.5.4.5 4.8
-
cytidine in 50 mM Tris-HCl buffer pH 7.5 at 25°C Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
3.5.4.5 metabolism CDA is a pyrimidine salvage pathway enzyme that recycles cytidine and 20-deoxycytidine for uridine and 2’-deoxyuridine synthesis, respectively Mycobacterium tuberculosis