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Literature summary extracted from

  • Byrns, M.C.; Duan, L.; Lee, S.H.; Blair, I.A.; Penning, T.M.
    Aldo-keto reductase 1C3 expression in MCF-7 cells reveals roles in steroid hormone and prostaglandin metabolism that may explain its over-expression in breast cancer (2010), J. Steroid Biochem. Mol. Biol., 118, 177-187.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.1.1.239 medicine establishment of MCF-7 cells that stably express AKR1C3 to model its over-expression in breast cancer. AKR1C3 expression increases steroid conversion by MCF-7 cells, leading to a pro-estrogenic state. Estrone is reduced fastest by MCF-7 cells expressing AKR1C3 when compared to other substrates at 0.1M. MCF-7 cells expressing AKR1C3 cells proliferate three times faster than parental cells in response to estrone and 17-estradiol. MCF-7 cells expressing AKR1C3 cells also reduce PGD2, limiting its dehydration to form PGJ2 products Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.239 expression in MCF-7 cell Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.239 0.009
-
 estrone pH 7.0, 37°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.239 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.239 recombinant enzyme Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.239 MCF-7 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.239 4-androstenedione + NADPH + H+
-
 Homo sapiens testosterone + NADP+
-
 ?
1.1.1.239 estrone + NADPH + H+
-
 Homo sapiens 17beta-estradiol + NADP+
-
 ?
1.1.1.239 progesterone + NADPH + H+
-
 Homo sapiens 20alpha-hydroxyprogesterone + NADP+
-
 ?
1.1.1.239 prostaglandin D2 + NADPH + H+
-
 Homo sapiens 9alpha,11beta-prostaglandin F2 + NADP+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.239 0.0011
-
 estrone pH 7.0, 37°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.239 0.127
-
 estrone pH 7.0, 37°C Homo sapiens