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Literature summary extracted from

  • Constantinesco, F.; Motorin, Y.; Grosjean, H.
    Characterisation and enzymatic properties of tRNA(guanine 26, N2,N2)-dimethyltransferase (Trm1p) from Pyrococcus furiosus (1999), J. Mol. Biol., 291, 375-392.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.216 expression in Escherichia coli Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.216 0.003
-
 S-adenosyl-L-methionine pH 7.2, 50°C Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.216 45700
-
 calculated from sequence Pyrococcus furiosus
2.1.1.216 49000
-
 gel filtration Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.216 2 S-adenosyl-L-methionine + guanine26 in tRNA Pyrococcus furiosus the efficient dimethylation of guanine26 requires the presence of base-pairs C11*G24 and G10*C25 and a variable loop of five bases within a correct 3D-core of the tRNA molecule. These identity elements probably ensure the correct presentation of methylated m2G26 to the enzyme for the attachment of the second methyl group 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNA
-
 ?
2.1.1.216 S-adenosyl-L-methionine + guanine26 in tRNA Pyrococcus furiosus
-
 S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
 ?
2.1.1.216 S-adenosyl-L-methionine + N2-methylguanine26 in tRNA Pyrococcus furiosus the efficient dimethylation of guanine26 requires the presence of base-pairs C11*G24 and G10*C25 and a variable loop of five bases within a correct 3D-core of the tRNA molecule. These identity elements probably ensure the correct presentation of methylated m2G26 to the enzyme for the attachment of the second methyl group S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.216 Pyrococcus furiosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.216 recombinant enzyme (pfTrm1p) with a His6-tag at the N-terminus Pyrococcus furiosus

Storage Stability

EC Number Storage Stability Organism
2.1.1.216 4°C, the purified enzyme can be stored for several months without loss of activity Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.216 2 S-adenosyl-L-methionine + guanine26 in tRNA the efficient dimethylation of guanine26 requires the presence of base-pairs C11*G24 and G10*C25 and a variable loop of five bases within a correct 3D-core of the tRNA molecule. These identity elements probably ensure the correct presentation of methylated m2G26 to the enzyme for the attachment of the second methyl group Pyrococcus furiosus 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNA
-
 ?
2.1.1.216 2 S-adenosyl-L-methionine + guanine26 in tRNA the efficient dimethylation of guanine26 requires the presence of base-pairs C11*G24 and G10*C25 and a variable loop of five bases within a correct 3D-core of the tRNA molecule. These identity elements probably ensure the correct presentation of methylated m2G26 to the enzyme for the attachment of the second methyl group. The methylated intermediate, and the enzyme dissociates from its tRNA substrate between the two consecutive methylation reactions Pyrococcus furiosus 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNA
-
 ?
2.1.1.216 2 S-adenosyl-L-methionine + guanine26 in tRNAPhe tRNAPhe from yeast, the efficient dimethylation of guanine26 requires the presence of base-pairs C11*G24 and G10*C25 and a variable loop of five bases within a correct 3D-core of the tRNA molecule. These identity elements probably ensure the correct presentation of methylated m2G26 to the enzyme for the attachment of the second methyl group Pyrococcus furiosus 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNAPhe
-
 ?
2.1.1.216 S-adenosyl-L-methionine + guanine26 in tRNA
-
 Pyrococcus furiosus S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
 ?
2.1.1.216 S-adenosyl-L-methionine + guanine26 in tRNAPhe tRNAPhe from yeast Pyrococcus furiosus S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNAPhe
-
 ?
2.1.1.216 S-adenosyl-L-methionine + N2-methylguanine26 in tRNA the efficient dimethylation of guanine26 requires the presence of base-pairs C11*G24 and G10*C25 and a variable loop of five bases within a correct 3D-core of the tRNA molecule. These identity elements probably ensure the correct presentation of methylated m2G26 to the enzyme for the attachment of the second methyl group Pyrococcus furiosus S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
2.1.1.216 S-adenosyl-L-methionine + N2-methylguanine26 in tRNA the efficient dimethylation of guanine26 requires the presence of base-pairs C11*G24 and G10*C25 and a variable loop of five bases within a correct 3D-core of the tRNA molecule. These identity elements probably ensure the correct presentation of methylated m2G26 to the enzyme for the attachment of the second methyl group. The methylated intermediate, and the enzyme dissociates from its tRNA substrate between the two consecutive methylation reactions Pyrococcus furiosus S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
2.1.1.216 S-adenosyl-L-methionine + N2-methylguanine26 in tRNAPhe tRNAPhe from yeast, the efficient dimethylation of guanine26 requires the presence of base-pairs C11*G24 and G10*C25 and a variable loop of five bases within a correct 3D-core of the tRNA molecule. These identity elements probably ensure the correct presentation of methylated m2G26 to the enzyme for the attachment of the second methyl group Pyrococcus furiosus S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNAPhe
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.216 monomer
-
 Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
2.1.1.216 Trm1p
-
 Pyrococcus furiosus
2.1.1.216 tRNA(guanine26,N2,N2)-dimethyltransferase
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.216 50
-
 assay at Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.216 80
-
 2 h: stable Pyrococcus furiosus
2.1.1.216 90
-
 2 h: 30% loss of activity Pyrococcus furiosus
2.1.1.216 95
-
 t1/2: 2 h Pyrococcus furiosus