BRENDA - Enzyme Database show

Purification and some properties of carboxynorspermidine synthase participating in a novel biosynthetic pathway for norspermidine inVibrio alginolyticus

Nakao, H.; Shinoda, S.; Yamamoto, S.; J. Gen. Microbiol. 137, 1737-1742 (1991)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.4.1.B4
DTT
without addition 7% of the maximum activity detected in the purified enzyme, 14fold activiation is observed at 20 mM
Vibrio alginolyticus
1.4.1.B4
additional information
1 mM EDTA shows no stimulatory effect on enzyme activity
Vibrio alginolyticus
1.5.1.43
dithiothreitol
about 14fold increase of activity at 20 mM. Without addition of dithiothreitol to the assay mixture, only 7% of the maximum activity is detected in the purified enzyme
Vibrio alginolyticus
1.5.1.43
additional information
EDTA (1 mM) shows no significant stimulatory effect on enzyme activity
Vibrio alginolyticus
General Stability
EC Number
General Stability
Organism
1.4.1.B4
no significant stabilization by addition of NADPH. 1 mM DTT has a protective effect on the enzyme during purification and storage. Freezing and thawing results in almost complete loss of activity
Vibrio alginolyticus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.4.1.B4
1,2-diaminopropane
10 mM, causes 10-20% inhibition of carboxynorspermidine formation
Vibrio alginolyticus
1.4.1.B4
cadaverine
10 mM, causes 10-20% inhibition of carboxynorspermidine formation
Vibrio alginolyticus
1.4.1.B4
ethylenediamine
10 mM, causes 10-20% inhibition of carboxynorspermidine formation
Vibrio alginolyticus
1.4.1.B4
ethylmaleimide
5 mM, in presence of 20 mM, 83% inhibition
Vibrio alginolyticus
1.4.1.B4
iodoacetamide
5 mM, in presence of 20 mM, 24% inhibition
Vibrio alginolyticus
1.4.1.B4
additional information
no inhibitory effect of NAD+, ATP, and spermidine at 5 or 10 mM. No effect of 1 mM Na+ or K+. No evidence for inhibitory effect of metal cations such as Ca2+, Mg2+, Fe3+, Fe2+, Cu+, Cu2+, Mn2+, and Zn2+. EDTA, 1 mM no inhibition
Vibrio alginolyticus
1.4.1.B4
NADP+
5 mM, 51% inhibition
Vibrio alginolyticus
1.4.1.B4
norspermidine
10 mM, 10% inhibition
Vibrio alginolyticus
1.5.1.43
iodoacetamide
24% inhibition at 5 mM
Vibrio alginolyticus
1.5.1.43
additional information
EDTA (1 mM) shows no significant inhibitory effect on enzyme activity; NAD+, ATP and SPD are not inhibitory at 5 mM
Vibrio alginolyticus
1.5.1.43
N-ethylmaleimide
83% inhibition at 5 mM
Vibrio alginolyticus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4.1.B4
additional information
-
additional information
the Km for the Schiff base is 4.68 mM, the intermediate formed from L-aspartic 4-semialdehyde and 1,3-diaminopropane, which is reduced to carboxynorspermidine
Vibrio alginolyticus
1.4.1.B4
1.51
-
NADPH
pH 7.5, 37C, with a Vmax of 31 micromol carboxynorspermidine/min/mg protein formed
Vibrio alginolyticus
1.4.1.B4
2.97
-
NADH
pH 7.5, 37C, with a Vmax of 13.5 micromol carboxynorspermidine/min/mg protein formed
Vibrio alginolyticus
1.4.1.B4
4.68
-
L-aspartic 4-semialdehyde
pH 7.5, 37C, Vmax: 35 micromol/min/mg carboxynorspermidine
Vibrio alginolyticus
1.5.1.43
1.51
-
NADPH
at pH 7.5 and 37C
Vibrio alginolyticus
1.5.1.43
2.97
-
NADH
at pH 7.5 and 37C
Vibrio alginolyticus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.4.1.B4
additional information
no evidence for activity being dependent on metal ions such as Ca2+, Mg2+, Fe3+, Fe2+, Cu+, Cu2+, Mn2+, and Zn2+
Vibrio alginolyticus
1.5.1.43
additional information
Ca2+, Mg2+, Fe3+, Fe2+, Cu+, Cu2+, Mn2+, Zn2+, Na+ and K+ at 1 M have no effect on enzyme activity
Vibrio alginolyticus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.4.1.B4
45100
-
2 * 45100, SDS-PAGE, indicates that the native enzyme is composed of two subunits of similar size
Vibrio alginolyticus
1.4.1.B4
93500
-
gel filtration
Vibrio alginolyticus
1.5.1.43
45100
-
2 * 45100, SDS-PAGE
Vibrio alginolyticus
1.5.1.43
93500
-
gel filtration
Vibrio alginolyticus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.4.1.B4
Vibrio alginolyticus
-
-
-
1.5.1.43
Vibrio alginolyticus
-
-
-
1.5.1.43
Vibrio alginolyticus ATCC 17749
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.4.1.B4
to homogeneity, yield 33%, 1800fold purified, ammonium sulfate precipitation, DEAE-Sepharose CL-6B, hydroxyapatite, and Blue Sepharose CL-6B chromatography
Vibrio alginolyticus
1.5.1.43
ammonium sulfate precipitation, Blue Sepharose CL-6B column chromatography, DEAE-Sepharose column chromatography, and hydroxyapatite column chromatography
Vibrio alginolyticus
Reaction
EC Number
Reaction
Commentary
Organism
1.4.1.B4
L-aspartic 4-semialdehyde + 1,3-diaminopropane + NADPH + H+ = carboxynorspermidine + NADP+ + H2O
i.e. (3-((3-aminopropyl)amino)propyl)carbamate (carboxynorspermidine) and (2S)-2-amino-4-oxobutanoate (L-aspartic 4-semialdehyde) via a nicotinamide-nucleotide reduction of the Schiff base H2N(CH2)3=CHCH2CH(NH2)COOH, formed from L-aspartic beta-semialdehyde, an intermediate in the novel pathway for norspermidine biosynthesis
Vibrio alginolyticus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.4.1.B4
additional information
-
in the presence of 5 mM nospermidine, specific activity is reduced by 70%
Vibrio alginolyticus
1.4.1.B4
0.0039
-
addition of 5 mM norspermidine to the growth medium, 27% compared to the specific activity without addition, pH 7.5, 37C
Vibrio alginolyticus
1.4.1.B4
0.0108
-
addition of 5 mM spermidine to the growth medium, 74% compared to the specific activity without addition, pH 7.5, 37C
Vibrio alginolyticus
1.4.1.B4
0.01455
-
without addition of norspermidine or spermidine to the growth medium, pH 7.5, 37C
Vibrio alginolyticus
1.4.1.B4
31
-
micromol carboxynorspermidine/min-1/mg protein, pH 7.5, 37C
Vibrio alginolyticus
1.5.1.43
0.0167
-
crude enzyme, at pH 7.5 and 37C
Vibrio alginolyticus
1.5.1.43
31
-
after 1856fold purification, at pH 7.5 and 37C
Vibrio alginolyticus
Storage Stability
EC Number
Storage Stability
Organism
1.4.1.B4
4C, protein concentration of 1.6 mg/ml in 20 mM Tris-HCl, pH 7.5, 1 mM DTT and 0.02% NaN3, lost of about 15% of its activity after one week
Vibrio alginolyticus
1.5.1.43
4C, in 20 mM Tris/HCl, pH 7.5, containing 1 mM dithiothreitol and 0.02% (v/v) NaN3, 1 week, 15% loss of activity
Vibrio alginolyticus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.1.B4
carboxynorspermidine + NAD+ + H2O
NADH is a much poorer cofactor than NADPH
712623
Vibrio alginolyticus
L-aspartic 4-semialdehyde + 1,3-diaminopropane + NADH + H+
-
-
-
ir
1.4.1.B4
carboxynorspermidine + NAD+ + H2O
NADH is a much poorer cofactor than NADPH
712623
Vibrio alginolyticus ATCC 17749
L-aspartic 4-semialdehyde + 1,3-diaminopropane + NADH + H+
-
-
-
ir
1.4.1.B4
carboxynorspermidine + NADP+ + H2O
i.e. (3-((3-aminopropyl)amino)propyl)carbamate, incubation of the purified enzyme in the range of pH 6-8, with cofactor NADP+ or NAD+ concentrations up to 5 mM and substrate concentrations of carboxynorspermidine up to 10 mM, no reversible activity is observed
712623
Vibrio alginolyticus
L-aspartic 4-semialdehyde + 1,3-diaminopropane + NADPH + H+
i.e. (2S)-2-amino-4-oxobutanoate
-
-
ir
1.4.1.B4
carboxynorspermidine + NADP+ + H2O
i.e. (3-((3-aminopropyl)amino)propyl)carbamate, incubation of the purified enzyme in the range of pH 6-8, with cofactor NADP+ or NAD+ concentrations up to 5 mM and substrate concentrations of carboxynorspermidine up to 10 mM, no reversible activity is observed
712623
Vibrio alginolyticus ATCC 17749
L-aspartic 4-semialdehyde + 1,3-diaminopropane + NADPH + H+
i.e. (2S)-2-amino-4-oxobutanoate
-
-
ir
1.4.1.B4
carboxyspermidine + NADP+ + H2O
i.e. 3-[(4-aminobutyl)amino]-L-alanine, slightly active with putrescine, ca. 7% of the rate compared to 1,3-diaminopropane
712623
Vibrio alginolyticus
L-aspartic 4-semialdehyde + putrescine + NADPH + H+
-
-
-
ir
1.4.1.B4
carboxyspermidine + NADP+ + H2O
i.e. 3-[(4-aminobutyl)amino]-L-alanine, slightly active with putrescine, ca. 7% of the rate compared to 1,3-diaminopropane
712623
Vibrio alginolyticus ATCC 17749
L-aspartic 4-semialdehyde + putrescine + NADPH + H+
-
-
-
ir
1.4.1.B4
additional information
no activity with ethylenediamine, cadaverine, and D-aspartic beta-semialdehyde
712623
Vibrio alginolyticus
?
-
-
-
-
1.4.1.B4
additional information
no activity with ethylenediamine, cadaverine, and D-aspartic beta-semialdehyde
712623
Vibrio alginolyticus ATCC 17749
?
-
-
-
-
1.5.1.43
L-aspartate 4-semialdehyde + propane-1,3-diamine + NADH + H+
NADH is a much poorer cofactor than NADPH
712623
Vibrio alginolyticus
carboxynorspermidine + H2O + NAD+
-
-
-
?
1.5.1.43
L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H+
-
712623
Vibrio alginolyticus
carboxynorspermidine + H2O + NADP+
-
-
-
?
1.5.1.43
L-aspartate 4-semialdehyde + putrescine + NADPH + H+
7% activity with putrescine compared to propane-1,3-diamine
712623
Vibrio alginolyticus
carboxyspermidine + H2O + NADP+
-
-
-
?
1.5.1.43
additional information
does not react with ethylenediamine, cadaverine and D-aspartate 4-semialdehyde
712623
Vibrio alginolyticus
?
-
-
-
-
1.5.1.43
additional information
does not react with ethylenediamine, cadaverine and D-aspartate 4-semialdehyde
712623
Vibrio alginolyticus ATCC 17749
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
1.4.1.B4
dimer
2 * 45100, SDS-PAGE, indicates that the native enzyme is composed of two subunits of similar size
Vibrio alginolyticus
1.5.1.43
homodimer
2 * 45100, SDS-PAGE
Vibrio alginolyticus
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.4.1.B4
37
-
-
Vibrio alginolyticus
1.5.1.43
37
-
-
Vibrio alginolyticus
Temperature Range [C]
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
1.4.1.B4
30
45
78 and 59% of the maximal activity at 30 and 45C, respectively, no activity at 50C
Vibrio alginolyticus
1.5.1.43
30
50
78% and 59% of the maximum activity remain at 30C and 45C, respectively, but no activity is observed above 50C
Vibrio alginolyticus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.4.1.B4
7.25
7.5
-
Vibrio alginolyticus
1.5.1.43
7.3
7.5
-
Vibrio alginolyticus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.4.1.B4
6.25
8
18 and 85% of the maximal acticity at pH 6.25 and 8.0, respectively
Vibrio alginolyticus
1.5.1.43
6.3
8
85% and 18% of the maximum activity are observed at pH 8.0 and 6.3, respectively
Vibrio alginolyticus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.4.1.B4
6.5
-
the enzyme is unstable in buffers below pH 6.5, at pH 6.5 50% loss of activity is observed within 12 h at 4C
Vibrio alginolyticus
1.5.1.43
6.5
-
the enzyme is unstable in buffers of pH below 6.5. At pH 6.5, 50% loss of activity is observed within 12 h at 4C
Vibrio alginolyticus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.4.1.B4
NADH
conmpletely inactive unless NADPH or NADH are added, NADH is a much poorer cofactor than NADPH
Vibrio alginolyticus
1.4.1.B4
NADPH
conmpletely inactive unless NADPH or NADH are added, NADH is a much poorer cofactor than NADPH
Vibrio alginolyticus
1.5.1.43
NADH
NADH is a much poorer cofactor than NADPH
Vibrio alginolyticus
1.5.1.43
NADPH
-
Vibrio alginolyticus
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.4.1.B4
Vibrio alginolyticus
isoelectric focusing and chromatofocusing
4.3
4.2
1.5.1.43
Vibrio alginolyticus
isoelectric focusing
4.3
4.2
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.4.1.B4
DTT
without addition 7% of the maximum activity detected in the purified enzyme, 14fold activiation is observed at 20 mM
Vibrio alginolyticus
1.4.1.B4
additional information
1 mM EDTA shows no stimulatory effect on enzyme activity
Vibrio alginolyticus
1.5.1.43
dithiothreitol
about 14fold increase of activity at 20 mM. Without addition of dithiothreitol to the assay mixture, only 7% of the maximum activity is detected in the purified enzyme
Vibrio alginolyticus
1.5.1.43
additional information
EDTA (1 mM) shows no significant stimulatory effect on enzyme activity
Vibrio alginolyticus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.4.1.B4
NADH
conmpletely inactive unless NADPH or NADH are added, NADH is a much poorer cofactor than NADPH
Vibrio alginolyticus
1.4.1.B4
NADPH
conmpletely inactive unless NADPH or NADH are added, NADH is a much poorer cofactor than NADPH
Vibrio alginolyticus
1.5.1.43
NADH
NADH is a much poorer cofactor than NADPH
Vibrio alginolyticus
1.5.1.43
NADPH
-
Vibrio alginolyticus
General Stability (protein specific)
EC Number
General Stability
Organism
1.4.1.B4
no significant stabilization by addition of NADPH. 1 mM DTT has a protective effect on the enzyme during purification and storage. Freezing and thawing results in almost complete loss of activity
Vibrio alginolyticus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.4.1.B4
1,2-diaminopropane
10 mM, causes 10-20% inhibition of carboxynorspermidine formation
Vibrio alginolyticus
1.4.1.B4
cadaverine
10 mM, causes 10-20% inhibition of carboxynorspermidine formation
Vibrio alginolyticus
1.4.1.B4
ethylenediamine
10 mM, causes 10-20% inhibition of carboxynorspermidine formation
Vibrio alginolyticus
1.4.1.B4
ethylmaleimide
5 mM, in presence of 20 mM, 83% inhibition
Vibrio alginolyticus
1.4.1.B4
iodoacetamide
5 mM, in presence of 20 mM, 24% inhibition
Vibrio alginolyticus
1.4.1.B4
additional information
no inhibitory effect of NAD+, ATP, and spermidine at 5 or 10 mM. No effect of 1 mM Na+ or K+. No evidence for inhibitory effect of metal cations such as Ca2+, Mg2+, Fe3+, Fe2+, Cu+, Cu2+, Mn2+, and Zn2+. EDTA, 1 mM no inhibition
Vibrio alginolyticus
1.4.1.B4
NADP+
5 mM, 51% inhibition
Vibrio alginolyticus
1.4.1.B4
norspermidine
10 mM, 10% inhibition
Vibrio alginolyticus
1.5.1.43
iodoacetamide
24% inhibition at 5 mM
Vibrio alginolyticus
1.5.1.43
additional information
EDTA (1 mM) shows no significant inhibitory effect on enzyme activity; NAD+, ATP and SPD are not inhibitory at 5 mM
Vibrio alginolyticus
1.5.1.43
N-ethylmaleimide
83% inhibition at 5 mM
Vibrio alginolyticus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4.1.B4
additional information
-
additional information
the Km for the Schiff base is 4.68 mM, the intermediate formed from L-aspartic 4-semialdehyde and 1,3-diaminopropane, which is reduced to carboxynorspermidine
Vibrio alginolyticus
1.4.1.B4
1.51
-
NADPH
pH 7.5, 37C, with a Vmax of 31 micromol carboxynorspermidine/min/mg protein formed
Vibrio alginolyticus
1.4.1.B4
2.97
-
NADH
pH 7.5, 37C, with a Vmax of 13.5 micromol carboxynorspermidine/min/mg protein formed
Vibrio alginolyticus
1.4.1.B4
4.68
-
L-aspartic 4-semialdehyde
pH 7.5, 37C, Vmax: 35 micromol/min/mg carboxynorspermidine
Vibrio alginolyticus
1.5.1.43
1.51
-
NADPH
at pH 7.5 and 37C
Vibrio alginolyticus
1.5.1.43
2.97
-
NADH
at pH 7.5 and 37C
Vibrio alginolyticus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.4.1.B4
additional information
no evidence for activity being dependent on metal ions such as Ca2+, Mg2+, Fe3+, Fe2+, Cu+, Cu2+, Mn2+, and Zn2+
Vibrio alginolyticus
1.5.1.43
additional information
Ca2+, Mg2+, Fe3+, Fe2+, Cu+, Cu2+, Mn2+, Zn2+, Na+ and K+ at 1 M have no effect on enzyme activity
Vibrio alginolyticus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.4.1.B4
45100
-
2 * 45100, SDS-PAGE, indicates that the native enzyme is composed of two subunits of similar size
Vibrio alginolyticus
1.4.1.B4
93500
-
gel filtration
Vibrio alginolyticus
1.5.1.43
45100
-
2 * 45100, SDS-PAGE
Vibrio alginolyticus
1.5.1.43
93500
-
gel filtration
Vibrio alginolyticus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.4.1.B4
to homogeneity, yield 33%, 1800fold purified, ammonium sulfate precipitation, DEAE-Sepharose CL-6B, hydroxyapatite, and Blue Sepharose CL-6B chromatography
Vibrio alginolyticus
1.5.1.43
ammonium sulfate precipitation, Blue Sepharose CL-6B column chromatography, DEAE-Sepharose column chromatography, and hydroxyapatite column chromatography
Vibrio alginolyticus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.4.1.B4
additional information
-
in the presence of 5 mM nospermidine, specific activity is reduced by 70%
Vibrio alginolyticus
1.4.1.B4
0.0039
-
addition of 5 mM norspermidine to the growth medium, 27% compared to the specific activity without addition, pH 7.5, 37C
Vibrio alginolyticus
1.4.1.B4
0.0108
-
addition of 5 mM spermidine to the growth medium, 74% compared to the specific activity without addition, pH 7.5, 37C
Vibrio alginolyticus
1.4.1.B4
0.01455
-
without addition of norspermidine or spermidine to the growth medium, pH 7.5, 37C
Vibrio alginolyticus
1.4.1.B4
31
-
micromol carboxynorspermidine/min-1/mg protein, pH 7.5, 37C
Vibrio alginolyticus
1.5.1.43
0.0167
-
crude enzyme, at pH 7.5 and 37C
Vibrio alginolyticus
1.5.1.43
31
-
after 1856fold purification, at pH 7.5 and 37C
Vibrio alginolyticus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.4.1.B4
4C, protein concentration of 1.6 mg/ml in 20 mM Tris-HCl, pH 7.5, 1 mM DTT and 0.02% NaN3, lost of about 15% of its activity after one week
Vibrio alginolyticus
1.5.1.43
4C, in 20 mM Tris/HCl, pH 7.5, containing 1 mM dithiothreitol and 0.02% (v/v) NaN3, 1 week, 15% loss of activity
Vibrio alginolyticus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.1.B4
carboxynorspermidine + NAD+ + H2O
NADH is a much poorer cofactor than NADPH
712623
Vibrio alginolyticus
L-aspartic 4-semialdehyde + 1,3-diaminopropane + NADH + H+
-
-
-
ir
1.4.1.B4
carboxynorspermidine + NAD+ + H2O
NADH is a much poorer cofactor than NADPH
712623
Vibrio alginolyticus ATCC 17749
L-aspartic 4-semialdehyde + 1,3-diaminopropane + NADH + H+
-
-
-
ir
1.4.1.B4
carboxynorspermidine + NADP+ + H2O
i.e. (3-((3-aminopropyl)amino)propyl)carbamate, incubation of the purified enzyme in the range of pH 6-8, with cofactor NADP+ or NAD+ concentrations up to 5 mM and substrate concentrations of carboxynorspermidine up to 10 mM, no reversible activity is observed
712623
Vibrio alginolyticus
L-aspartic 4-semialdehyde + 1,3-diaminopropane + NADPH + H+
i.e. (2S)-2-amino-4-oxobutanoate
-
-
ir
1.4.1.B4
carboxynorspermidine + NADP+ + H2O
i.e. (3-((3-aminopropyl)amino)propyl)carbamate, incubation of the purified enzyme in the range of pH 6-8, with cofactor NADP+ or NAD+ concentrations up to 5 mM and substrate concentrations of carboxynorspermidine up to 10 mM, no reversible activity is observed
712623
Vibrio alginolyticus ATCC 17749
L-aspartic 4-semialdehyde + 1,3-diaminopropane + NADPH + H+
i.e. (2S)-2-amino-4-oxobutanoate
-
-
ir
1.4.1.B4
carboxyspermidine + NADP+ + H2O
i.e. 3-[(4-aminobutyl)amino]-L-alanine, slightly active with putrescine, ca. 7% of the rate compared to 1,3-diaminopropane
712623
Vibrio alginolyticus
L-aspartic 4-semialdehyde + putrescine + NADPH + H+
-
-
-
ir
1.4.1.B4
carboxyspermidine + NADP+ + H2O
i.e. 3-[(4-aminobutyl)amino]-L-alanine, slightly active with putrescine, ca. 7% of the rate compared to 1,3-diaminopropane
712623
Vibrio alginolyticus ATCC 17749
L-aspartic 4-semialdehyde + putrescine + NADPH + H+
-
-
-
ir
1.4.1.B4
additional information
no activity with ethylenediamine, cadaverine, and D-aspartic beta-semialdehyde
712623
Vibrio alginolyticus
?
-
-
-
-
1.4.1.B4
additional information
no activity with ethylenediamine, cadaverine, and D-aspartic beta-semialdehyde
712623
Vibrio alginolyticus ATCC 17749
?
-
-
-
-
1.5.1.43
L-aspartate 4-semialdehyde + propane-1,3-diamine + NADH + H+
NADH is a much poorer cofactor than NADPH
712623
Vibrio alginolyticus
carboxynorspermidine + H2O + NAD+
-
-
-
?
1.5.1.43
L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H+
-
712623
Vibrio alginolyticus
carboxynorspermidine + H2O + NADP+
-
-
-
?
1.5.1.43
L-aspartate 4-semialdehyde + putrescine + NADPH + H+
7% activity with putrescine compared to propane-1,3-diamine
712623
Vibrio alginolyticus
carboxyspermidine + H2O + NADP+
-
-
-
?
1.5.1.43
additional information
does not react with ethylenediamine, cadaverine and D-aspartate 4-semialdehyde
712623
Vibrio alginolyticus
?
-
-
-
-
1.5.1.43
additional information
does not react with ethylenediamine, cadaverine and D-aspartate 4-semialdehyde
712623
Vibrio alginolyticus ATCC 17749
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.4.1.B4
dimer
2 * 45100, SDS-PAGE, indicates that the native enzyme is composed of two subunits of similar size
Vibrio alginolyticus
1.5.1.43
homodimer
2 * 45100, SDS-PAGE
Vibrio alginolyticus
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.4.1.B4
37
-
-
Vibrio alginolyticus
1.5.1.43
37
-
-
Vibrio alginolyticus
Temperature Range [C] (protein specific)
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
1.4.1.B4
30
45
78 and 59% of the maximal activity at 30 and 45C, respectively, no activity at 50C
Vibrio alginolyticus
1.5.1.43
30
50
78% and 59% of the maximum activity remain at 30C and 45C, respectively, but no activity is observed above 50C
Vibrio alginolyticus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.4.1.B4
7.25
7.5
-
Vibrio alginolyticus
1.5.1.43
7.3
7.5
-
Vibrio alginolyticus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.4.1.B4
6.25
8
18 and 85% of the maximal acticity at pH 6.25 and 8.0, respectively
Vibrio alginolyticus
1.5.1.43
6.3
8
85% and 18% of the maximum activity are observed at pH 8.0 and 6.3, respectively
Vibrio alginolyticus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.4.1.B4
6.5
-
the enzyme is unstable in buffers below pH 6.5, at pH 6.5 50% loss of activity is observed within 12 h at 4C
Vibrio alginolyticus
1.5.1.43
6.5
-
the enzyme is unstable in buffers of pH below 6.5. At pH 6.5, 50% loss of activity is observed within 12 h at 4C
Vibrio alginolyticus
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.4.1.B4
Vibrio alginolyticus
isoelectric focusing and chromatofocusing
4.3
4.2
1.5.1.43
Vibrio alginolyticus
isoelectric focusing
4.3
4.2
Expression
EC Number
Organism
Commentary
Expression
1.5.1.43
Vibrio alginolyticus
when Vibrio alginolyticus is grown in the presence of 5 mM norspermidine, the specific activity of the enzyme is reduced by about 70%
down
General Information
EC Number
General Information
Commentary
Organism
1.4.1.B4
physiological function
carboxynorspermidine synthase participates in the novel pathway for norspermidine biosynthesis, with two other enzymes, 2,4-diamonobutyrate deacroxylase and carboxynorspermidine decarboxylase
Vibrio alginolyticus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.4.1.B4
physiological function
carboxynorspermidine synthase participates in the novel pathway for norspermidine biosynthesis, with two other enzymes, 2,4-diamonobutyrate deacroxylase and carboxynorspermidine decarboxylase
Vibrio alginolyticus
Expression (protein specific)
EC Number
Organism
Commentary
Expression
1.5.1.43
Vibrio alginolyticus
when Vibrio alginolyticus is grown in the presence of 5 mM norspermidine, the specific activity of the enzyme is reduced by about 70%
down