Any feedback?
Literature summary extracted from
- Characterization of polyphenol oxidase from water caltrop (Trapa acornis Nakano) fruits (2010), J. Food Biochem., 34, 1125-1140.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.18.1 | CaCl2 | 373.5% relative activity at 5 mM | Trapa acornis |  |
| 1.14.18.1 | MnSO4 | 488.1% relative activity at 10 mM | Trapa acornis | |
| 1.14.18.1 | SDS | 342.4% relative activity at 10 mM | Trapa acornis | |
| 1.14.18.1 | Triton X-100 | 121% relative activity at 5 mM | Trapa acornis | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.18.1 | ascorbic acid | 17.6% residual activity at 1 mM | Trapa acornis | |
| 1.14.18.1 | Citric acid | 26% residual activity at 1 mM | Trapa acornis | |
| 1.14.18.1 | EDTA | 53.3% residual activity at 0.5 mM | Trapa acornis | |
| 1.14.18.1 | L-cysteine | 10.8% residual activity at 0.5 mM | Trapa acornis | |
| 1.14.18.1 | NaCl | 39.6% residual activity at 0.5 mM | Trapa acornis | |
| 1.14.18.1 | reduced glutathione | 42% residual activity at 1 mM | Trapa acornis | |
| 1.14.18.1 | Thiourea | 9.6% residual activity at 1 mM | Trapa acornis | |
| 1.14.18.1 | Triton X-100 | 42.5% residual activity at 10 mM | Trapa acornis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.18.1 | 15.4 | - |
catechol | at pH 6.0, 20°C | Trapa acornis | |
| 1.14.18.1 | 21.2 | - |
gallic acid | at pH 7.0, 30°C | Trapa acornis | |
| 1.14.18.1 | 57 | - |
pyrogallol | at pH 7.0, 30°C | Trapa acornis | |
| 1.14.18.1 | 77 | - |
L-tyrosine | at pH 5.8, 30°C | Trapa acornis | |
| 1.14.18.1 | 129.5 | - |
chlorogenic acid | at pH 7.0, 30°C | Trapa acornis | |
| 1.14.18.1 | 174.5 | - |
caffeic acid | at pH 6.0, 20°C | Trapa acornis | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.18.1 | chloroplast | - |
Trapa acornis | 9507 | - |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.18.1 | Trapa acornis | - |
water caltrop | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.18.1 | ammonium sulfate precipitation | Trapa acornis |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.18.1 | fruit | - |
Trapa acornis | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.18.1 | 2 catechol + O2 | most suitable substrate | Trapa acornis | 2 1,2-benzoquinone + 2 H2O | - |
? | |
| 1.14.18.1 | caffeic acid + O2 | 31% activity at 2.5 mM substrate concentration | Trapa acornis | caffeoyl quinone + H2O | - |
? | |
| 1.14.18.1 | chlorogenic acid + O2 | - |
Trapa acornis | ? | - |
? | |
| 1.14.18.1 | gallic acid + O2 | 39% activity at 2.5 mM substrate concentration | Trapa acornis | ? | - |
? | |
| 1.14.18.1 | L-tyrosine + O2 | - |
Trapa acornis | L-DOPA + H2O | - |
? | |
| 1.14.18.1 | pyrogallol + O2 | - |
Trapa acornis | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.18.1 | polyphenol oxidase | - |
Trapa acornis |
| 1.14.18.1 | PPO | - |
Trapa acornis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 20 | - |
for catechol and caffeic acid | Trapa acornis |
| 1.14.18.1 | 30 | - |
for gallic acid, chlorogenic acid, L-tyrosine and pyrogallol | Trapa acornis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 20 | 70 | PPO is stable at 20°C and 30°C for 20 min, and then the enzyme activity gradually decreases as time goes on. A decreased activity of 14.6% and 30% is observed after 50 min, respectively. The enzyme activity rapidly reduces at 40°C and 50°C as time goes on. After 50 min, the rudimental activities are only 43.7 and 10.6% of its original activity. The enzyme activity is nearly exhausted at 60°C and 70°C after 5 min | Trapa acornis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 5 | - |
for L-tyrosine | Trapa acornis |
| 1.14.18.1 | 6 | - |
for catechol and caffeic acid | Trapa acornis |
| 1.14.18.1 | 7 | - |
for gallic acid, chlorogenic acid and pyrogallol | Trapa acornis |
| 1.14.18.1 | 8 | - |
for L-tyrosine | Trapa acornis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 3 | 9 | enzyme activity decreases below pH 5.0 or above pH 8.0, however, the enzyme is still active at pH 3.0 and pH 9.0. PPO remains 77.96, 60.05 and 52.24% of its original activity between pH 5.0 and pH 7.0 using catechol, L-tyrosine and pyrogallol as substrates, respectively, and loses more than 57% of activity when using caffeic acid, gallic acid and chlorogenic acid as substrates at their optimum pH values | Trapa acornis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
