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Literature summary extracted from

  • Zalewski, W.; Galuszka, P.; Gasparis, S.; Orczyk, W.; Nadolska-Orczyk, A.
    Silencing of the HvCKX1 gene decreases the cytokinin oxidase/dehydrogenase level in barley and leads to higher plant productivity (2010), J. Exp. Bot., 61, 1839-1851.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.99.12 expressed in Escherichia coli strain DH5alpha Triticum aestivum
1.5.99.12 expressed in Escherichia coli strain DH5alpha Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
1.5.99.12 Hordeum vulgare Q94KI4 cultivars Golden Promise, Scarlett, Torka, and Kontesa
-
1.5.99.12 Triticum aestivum
-
cultivar Wanad
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.99.12 root
-
Triticum aestivum
-
1.5.99.12 root
-
Hordeum vulgare
-
1.5.99.12 seedling
-
Triticum aestivum
-
1.5.99.12 seedling
-
Hordeum vulgare
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.99.12 N6-(2-isopentenyl)adenine + 2,6-dichlorophenolindophenol + H2O
-
Triticum aestivum adenine + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol
-
?
1.5.99.12 N6-(2-isopentenyl)adenine + 2,6-dichlorophenolindophenol + H2O
-
Hordeum vulgare adenine + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol
-
?

Synonyms

EC Number Synonyms Comment Organism
1.5.99.12 CKX1
-
Triticum aestivum
1.5.99.12 CKX1
-
Hordeum vulgare
1.5.99.12 cytokinin oxidase/dehydrogenase
-
Triticum aestivum
1.5.99.12 cytokinin oxidase/dehydrogenase
-
Hordeum vulgare

Expression

EC Number Organism Comment Expression
1.5.99.12 Triticum aestivum highest levels of the CKX1 transcript are detected in spikes 0 days, 7 days, and 14 days after pollination and in the seedling roots up
1.5.99.12 Hordeum vulgare highest levels of the CKX1 transcript are detected in spikes 0 days, 7 days, and 14 days after pollination and in the seedling roots up

General Information

EC Number General Information Comment Organism
1.5.99.12 malfunction silencing of the CKX1 gene decreases the cytokinin oxidase/dehydrogenase level in barley and leads to higher plant productivity (number of seeds per plant) and root weight Triticum aestivum
1.5.99.12 malfunction silencing of the CKX1 gene decreases the cytokinin oxidase/dehydrogenase level in barley and leads to higher plant productivity (number of seeds per plant) and root weight Hordeum vulgare