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Literature summary extracted from

  • Nagano, T.; Kakegawa, A.; Yamakoshi, Y.; Tsuchiya, S.; Hu, J.C.; Gomi, K.; Arai, T.; Bartlett, J.D.; Simmer, J.P.
    Mmp-20 and Klk4 cleavage site preferences for amelogenin sequences (2009), J. Dent. Res., 88, 823-828.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.B6 extracellular
-
Sus scrofa
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.B6 Ca2+ required Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.B6 amelogenin + H2O Sus scrofa
-
amelogenin fragments
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.B6 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.B6 native enzyme from soft enamel-alkaline extract by anion exchange chromatography, ultrafiltration, and heparin affinity chromatography Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.B6 enamel secretory stage Sus scrofa
-
3.4.24.B6 tooth developing Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.B6 amelogenin + H2O
-
Sus scrofa amelogenin fragments
-
?
3.4.24.B6 amelogenin + H2O Mmp-20 cleaves amelogenin sequences after Pro162, Ser148, His62, Ala63, and Trp45. These cleavages generate all of the major cleavage products from tyrosine-rich amelogenin polypeptide that accumulate in porcine secretory stage enamel, i.e. the 23 kDa, 20 kDa, 13 kDa, 11 kDa, and 6 kDa amelogenins. And Mmp-20 cleaves leucine rich-amelogenin protein after Pro45 and Pro40, producing the two LRAP products, Mmp-20 cleavage site preferences, overview Sus scrofa amelogenin fragments
-
?
3.4.24.B6 casein + H2O zymography Sus scrofa ?
-
?
3.4.24.B6 additional information digestion of FRET-amelogenin peptides, labeled with 2-aminobenzoyl and 2,4-dinitrophenyl, by Mmp-20, overview Sus scrofa ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.B6 enamel protease
-
Sus scrofa
3.4.24.B6 MMP-20
-
Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.B6 37
-
assay at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.B6 7.4
-
assay at Sus scrofa

General Information

EC Number General Information Comment Organism
3.4.24.B6 physiological function the enzyme processes amelogenin to generate the major cleavage products that accumulate during the secretory stage of amelogenesis Sus scrofa