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Literature summary extracted from

  • Mohamed Ali, E.
    Purification and characterization of Vigna unguiculata cultivar asparaginase (2009), J. Biol. Res., 11, 29-36.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.1 Ba2+ 5 mM, 37°C, 25% relative activity compared to the activity in absence of metal cations Vigna unguiculata
3.5.1.1 Hg2+ 5 mM, 37°C, 20% relative activity compared to the activity in absence of metal cations Vigna unguiculata
3.5.1.1 Mn2+ 5 mM, 37°C, 42% relative activity compared to the activity in absence of metal cations Vigna unguiculata
3.5.1.1 Zn2+ 5 mM, 37°C, 36% relative activity compared to the activity in absence of metal cations Vigna unguiculata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.1 1.25
-
 L-asparagine pH 8.0, 37°C Vigna unguiculata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.1 Co2+ 5 mM, 37°C, 134% relative activity compared to the activity in absence of metal cations Vigna unguiculata
3.5.1.1 Ni2+ 5 mM, 37°C, 136% relative activity compared to the activity in absence of metal cations Vigna unguiculata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.1 35000
-
 2 * 35000, SDS-PAGE, indicates this enzyme has two similar subunits Vigna unguiculata
3.5.1.1 70000
-
 gel filtration Vigna unguiculata

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Vigna unguiculata
-
 various cultivars: Kareem 7, Dokki 331, Kafer El-Sheikh 1, Kaha 1, and Fodder
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1 all three forms ASPG I, ASPG II, and ASPG II are purified, ASPG II shows highest specific activity, purity 9.27% and 36% recovery Vigna unguiculata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.1 seed germinating and non-germinating seeds are studied, highest specific activity in germinating seeds in all cultivars Vigna unguiculata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.1 14.3
-
 cultivar Dokki 331, non-germinating seeds, pH 8.0, 37°C Vigna unguiculata
3.5.1.1 16.8
-
 cultivar Kaha 1, non-germinating seeds, pH 8.0, 37°C Vigna unguiculata
3.5.1.1 18.5
-
 cultivar Kareem 7, non-germinating seeds, pH 8.0, 37°C Vigna unguiculata
3.5.1.1 24.3
-
 cultivar Kafer El-Sheikh 1, non-germinating seeds, pH 8.0, 37°C Vigna unguiculata
3.5.1.1 32.3
-
 cultivar Fodder, non-germinating seeds, pH 8.0, 37°C Vigna unguiculata
3.5.1.1 49
-
 cultivar Dokki 331, germinating seeds, pH 8.0, 37°C Vigna unguiculata
3.5.1.1 63
-
 cultivar Kaha 1, germinating seeds, pH 8.0, 37°C Vigna unguiculata
3.5.1.1 74
-
 cultivar Kareem 7, germinating seeds, pH 8.0, 37°C Vigna unguiculata
3.5.1.1 87
-
 cultivar Kafer El-Sheikh 1, germinating seeds, pH 8.0, 37°C Vigna unguiculata
3.5.1.1 105
-
 cultivar Fodder, non-germinating seeds, pH 8.0, 37°C Vigna unguiculata
3.5.1.1 969
-
 ASPG II, from germinating seeds, cultivar Fodder, pH 8.0, 37°C Vigna unguiculata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 L-asparagine + H2O
-
 Vigna unguiculata L-aspartate + NH3
-
 ?
3.5.1.1 L-aspartyl hydroxamate + H2O 60% relative activity compared to L-asparagine as substrate Vigna unguiculata L-aspartate + hydroxylamine
-
 ?
3.5.1.1 L-glutamine + H2O 36% relative activity compared to L-asparagine as substrate Vigna unguiculata L-glutamate + NH3
-
 ?
3.5.1.1 L-glutamyl hydroxamate + H2O 16% relative activity compared to L-asparagine as substrate Vigna unguiculata L-glutamate + hydroxylamine
-
 ?
3.5.1.1 additional information no activity with D-asparagine as substrate Vigna unguiculata ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.1 dimer 2 * 35000, SDS-PAGE, indicates this enzyme has two similar subunits Vigna unguiculata

Synonyms

EC Number Synonyms Comment Organism
3.5.1.1 ASPG II three forms, ASPG I, ASPG II, and ASPG III. ASPG II shows the highest specific activity Vigna unguiculata
3.5.1.1 L-asparaginase
-
 Vigna unguiculata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.1 37
-
 assay at Vigna unguiculata
3.5.1.1 40
-
-
 Vigna unguiculata

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.1 10 60 20% relative activity at 10 and 60°C Vigna unguiculata

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.1 40
-
 stable up to 40°C followed by sharp decline Vigna unguiculata
3.5.1.1 90
-
 loses about 95% activity at 90°C Vigna unguiculata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.1 8
-
 phosphate buffer Vigna unguiculata

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.1 5 9 20% relative activity at pH 5 and 40% relative activity at pH 9 Vigna unguiculata