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Literature summary extracted from

  • Yu, Q.; Ghisla, S.; Hirschberg, J.; Mann, V.; Beyer, P.
    Plant carotene cis-trans isomerase CRTISO: a new member of the FADred-dependent flavopreoteins catalyzing non-redox reactions (2011), J. Biol. Chem., 286, 8666-8676.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.2.1.13 additional information the enzyme requires anaerobic conditions for activity Solanum lycopersicum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.2.1.13
-
 Solanum lycopersicum

Organism

EC Number Organism UniProt Comment Textmining
5.2.1.13 Solanum lycopersicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.2.1.13
-
 Solanum lycopersicum

Reaction

EC Number Reaction Comment Organism Reaction ID
5.2.1.13 7,9,7',9'-tetracis-lycopene = all-trans-lycopene the mechanism of isomerization relies on acid-base catalysis Solanum lycopersicum
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.2.1.13 7,9,7',9'-tetracis-lycopene
-
 Solanum lycopersicum prolycopene all-trans-lycopene is the main lycopene species formed, accompanied by certain amounts of newly formed cis isomers ?

Synonyms

EC Number Synonyms Comment Organism
5.2.1.13 carotene cis-trans isomerase
-
 Solanum lycopersicum
5.2.1.13 CRTISO
-
 Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2.1.13 5.8 8 exhibits no pH-dependence in the range pH 5.8-8.0 Solanum lycopersicum

Cofactor

EC Number Cofactor Comment Organism Structure
5.2.1.13 FADH2 requires the reduced cofactor for activity. The enzyme requires anaerobic conditions for activity. Km: 0.00055 mM Solanum lycopersicum
5.2.1.13 FMNH2 can substitute for FADH2, with about 60% loss effectivity Solanum lycopersicum