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Literature summary extracted from

  • Beretta, M.; Gorren, A.C.; Wenzl, M.V.; Weis, R.; Russwurm, M.; Koesling, D.; Schmidt, K.; Mayer, B.
    Characterization of the East Asian variant of aldehyde dehydrogenase-2: bioactivation of nitroglycerin and effects of Alda-1 (2010), J. Biol. Chem., 285, 943-952.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.3 Alda-1 i.e. N-(1,3-benzodioxol-5-ylmethyl)-2,6-dichlorobenzamide, 0.01 mM Alda-1 increases acetaldehyde oxidation by wild type ALDH2 and the Asian variant E487K approximately 1.5 and 6fold, respectively. Alda-1 stimulates established ALDH2 activities by improving NAD+ binding but does not improve the nitroglycerin binding affinity of the Asian variant E487K Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.3 expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.3 E487K the East Asian variant results in very low aldehyde dehydrogenase activity and catalyzes nitroglycerin denitration with about 7fold lower maximal rates than the wild type enzyme1 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.3 Alda-1 0.01 mM Alda-1 inhibits the apparent formaldehyde oxidation activity of the wild type enzyme by about 90% Homo sapiens
1.2.1.3 NAD+ pronounced inhibition occurs at above 5 mM Homo sapiens
1.2.1.3 nitroglycerin exposure of ALDH2 to nitroglycerin results in mechanism-based oxidative inactivation of the enzyme that is partially reversed by dithiothreitol Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.3 mitochondrion
-
 Homo sapiens 5739
-

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.3 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.3
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.3 acetaldehyde + NAD+ + H2O
-
 Homo sapiens acetate + NADH + H+
-
 ?
1.2.1.3 formaldehyde + NAD+ + H2O
-
 Homo sapiens formate + NADH + H+
-
 ?
1.2.1.3 nitroglycerin + NAD+ + H2O
-
 Homo sapiens 1,2-glyceryl dinitrate + NO + NADH + H+
-
 ?
1.2.1.3 propionaldehyde + NAD+ + H2O
-
 Homo sapiens propionate + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.3 aldehyde dehydrogenase-2
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.3 NAD+
-
 Homo sapiens