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Literature summary extracted from

  • Angelucci, F.; Dimastrogiovanni, D.; Boumis, G.; Brunori, M.; Miele, A.E.; Saccoccia, F.; Bellelli, A.
    Mapping the catalytic cycle of Schistosoma mansoni thioredoxin glutathione reductase by X-ray crystallography (2010), J. Biol. Chem., 285, 32557-32567.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.9 expressed in Escherichia coli Schistosoma mansoni

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.1.9 TGR alone or in complex with NADH and GSSG, sitting drop vapor diffusion method, using 0.1 M HEPES, pH 7.4, 20% PEG 3350, 0.2 M KI and 5 mM beta-mercaptoethanol or 0.2 M magnesium formate, 20% PEG 6000, 0.1 M Hepes, pH 7.0-7.5 and 5 mM beta-mercaptoethanol (TGR with reduced and ordered C-terminus) Schistosoma mansoni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.9 90000
-
 SDS-PAGE Schistosoma mansoni

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.9 Schistosoma mansoni Q962Y6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.9
-
 Schistosoma mansoni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.9 thioredoxin + NADP+
-
 Schistosoma mansoni thioredoxin disulfide + NADPH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.9 TGR
-
 Schistosoma mansoni
1.8.1.9 thioredoxin glutathione reductase this natural chimeric enzyme is a peculiar fusion of a glutaredoxin domain with a thioredoxin selenocysteine-containing reductase domain Schistosoma mansoni

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.9 FAD
-
 Schistosoma mansoni
1.8.1.9 NADP+
-
 Schistosoma mansoni