EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.14.12.17 | ascorbate | stimulates NOD activity, reduces the oxidized Cygb-NOD intermediate with a second order rate of 1000 M/s, Km is 0.25 mM | Homo sapiens | |
1.14.12.17 | cytochrome b5 | stimulates NOD activity, reduces the oxidized Cygb-NOD intermediate with a second order rate of 3 x 1000000 M/s, Km is 0.3 mM | Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.12.17 | functional expression of human cytoglobin in rat hepatocytes and effects on the cell NO metabolism, overview. Expression in Escherichia coli strain BL21(DE3) | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.12.17 | CO | a competitive inhibitor | Homo sapiens | |
1.14.12.17 | cyanide | causes slow inactivation | Homo sapiens | |
1.14.12.17 | additional information | anions competitively inhibit activity. Increasing the sodium phosphate concentration or including sodium phosphate or NaCl in a 25 mM sodium citrate buffer also competitively inhibits the activity and increases the Km value of ascorbate | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.12.17 | additional information | - |
additional information | Michaelis-Menten behavior of the Cygb-NOD activity with ascorbate | Homo sapiens | |
1.14.12.17 | 0.00002 | - |
O2 | pH 7.0, 37°C | Homo sapiens | |
1.14.12.17 | 0.00004 | - |
nitric oxide | pH 7.0, 37°C | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.12.17 | Homo sapiens | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.12.17 | hepatocyte | - |
Homo sapiens | - |
1.14.12.17 | liver | - |
Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.12.17 | 2 nitric oxide + 2 O2 + NADPH | via an Cygb-NOD intermediate, putative ascorbate-binding site in Cygb, overview | Homo sapiens | 2 nitrate + NADP+ + H+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.12.17 | Cygb | - |
Homo sapiens |
1.14.12.17 | cytoglobin | - |
Homo sapiens |
1.14.12.17 | nitric-oxide dioxygenase | - |
Homo sapiens |
1.14.12.17 | NO dioxygenase | - |
Homo sapiens |
1.14.12.17 | NOD | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.12.17 | 37 | - |
assay at | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.12.17 | 7 | - |
assay at | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.12.17 | NADPH | - |
Homo sapiens |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.12.17 | additional information | the enzyme is distinct from the microsomal NOD activity | Homo sapiens |