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Literature summary extracted from

  • Rather, L.J.; Knapp, B.; Haehnel, W.; Fuchs, G.
    Coenzyme A-dependent aerobic metabolism of benzoate via epoxide formation (2010), J. Biol. Chem., 285, 20615-20624.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.13.208 BoxC protein
-
 Aromatoleum evansii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.208 expressed in Escherichia coli DH5alpha cells Aromatoleum evansii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.208 Fe2+ contains iron Aromatoleum evansii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.77 54000
-
 2 * 54000 Aromatoleum evansii
1.14.13.208 54000
-
 1 * 54000, BoxB, SDS-PAGE Aromatoleum evansii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.208 benzoyl-CoA + NADPH + H+ + O2 Aromatoleum evansii
-
 2,3-epoxy-2,3-dihydrobenzoyl-CoA + NADP+ + H2O
-
 ?
1.14.13.208 benzoyl-CoA + NADPH + H+ + O2 Aromatoleum evansii KB740
-
 2,3-epoxy-2,3-dihydrobenzoyl-CoA + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.77 Aromatoleum evansii
-
-
-
1.2.1.77 Aromatoleum evansii KB740
-
-
-
1.14.13.208 Aromatoleum evansii
-
-
-
1.14.13.208 Aromatoleum evansii KB740
-
-
-
4.1.2.44 Aromatoleum evansii Q84HH6
-
-
4.1.2.44 Aromatoleum evansii KB740 (DSM Z6869) Q84HH6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.77
-
 Aromatoleum evansii
1.14.13.208 Strep-Tactin Superflow column chromatography Aromatoleum evansii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.77 3,4-dehydroadipyl-CoA semialdehyde + NADP+ + H2O
-
 Aromatoleum evansii 3,4-dehydroadipyl-CoA + NADPH + H+
-
 ?
1.2.1.77 3,4-dehydroadipyl-CoA semialdehyde + NADP+ + H2O
-
 Aromatoleum evansii KB740 3,4-dehydroadipyl-CoA + NADPH + H+
-
 ?
1.14.13.208 benzoyl-CoA + NADPH + H+ + O2
-
 Aromatoleum evansii 2,3-epoxy-2,3-dihydrobenzoyl-CoA + NADP+ + H2O
-
 ?
1.14.13.208 benzoyl-CoA + NADPH + H+ + O2
-
 Aromatoleum evansii KB740 2,3-epoxy-2,3-dihydrobenzoyl-CoA + NADP+ + H2O
-
 ?
4.1.2.44 2,3-dihydro-2,3-dihydroxybenzoyl-CoA + H2O 2,3-dihydro-2,3-dihydroxybenzoyl-CoA is also named 2,3-epoxybenzoyl-CoA Aromatoleum evansii 3,4-dehydroadipyl-CoA semialdehyde + formate
-
 ?
4.1.2.44 2,3-dihydro-2,3-dihydroxybenzoyl-CoA + H2O 2,3-dihydro-2,3-dihydroxybenzoyl-CoA is also named 2,3-epoxybenzoyl-CoA Aromatoleum evansii KB740 (DSM Z6869) 3,4-dehydroadipyl-CoA semialdehyde + formate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.77 homodimer 2 * 54000 Aromatoleum evansii
1.14.13.208 monomer 1 * 54000, BoxB, SDS-PAGE Aromatoleum evansii

Synonyms

EC Number Synonyms Comment Organism
1.2.1.77 BoxD
-
 Aromatoleum evansii
1.2.1.77 NADP+-dependent aldehyde dehydrogenase
-
 Aromatoleum evansii
1.14.13.208 benzoyl-CoA oxygenase
-
 Aromatoleum evansii
1.14.13.208 BoxAB
-
 Aromatoleum evansii
4.1.2.44 2,3-epoxybenzoyl-CoA dihydrolase
-
 Aromatoleum evansii
4.1.2.44 benzoyl-CoA-dihydrodiol lyase formerly termed Aromatoleum evansii
4.1.2.44 BoxC
-
 Aromatoleum evansii

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.77 NADP+
-
 Aromatoleum evansii
1.14.13.208 FAD
-
 Aromatoleum evansii
1.14.13.208 Fe-S center
-
 Aromatoleum evansii
1.14.13.208 NADPH
-
 Aromatoleum evansii