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Literature summary extracted from

  • Brocker, C.; Lassen, N.; Estey, T.; Pappa, A.; Cantore, M.; Orlova, V.V.; Chavakis, T.; Kavanagh, K.L.; Oppermann, U.; Vasiliou, V.
    Aldehyde dehydrogenase 7A1 (ALDH7A1) is a novel enzyme involved in cellular defense against hyperosmotic stress (2010), J. Biol. Chem., 285, 18452-18463.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.3 expressed in Sf9 insect cells, in CHO cells, in human renal proximal tubular epithelial (HK-2) cells, and in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.1.3 sitting drop vapor diffusion method, using 20% (w/v) polyethylene glycol 3350, 10% (v/v) ethylene glycol, 0.2 M NaBr, 0.1 M Bis-Tris propane (pH 6.6) Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.3 0.0053
-
 2-nonenal pH and temperature not specified in the publication Homo sapiens
1.2.1.3 0.0175
-
 octanal pH and temperature not specified in the publication Homo sapiens
1.2.1.3 0.0285
-
 n-nonanal pH and temperature not specified in the publication Homo sapiens
1.2.1.3 0.0391
-
 hexanal pH and temperature not specified in the publication Homo sapiens
1.2.1.3 0.0411
-
 Betaine aldehyde pH and temperature not specified in the publication Homo sapiens
1.2.1.3 0.169
-
 alpha-aminoadipate semialdehyde pH and temperature not specified in the publication Homo sapiens
1.2.1.3 0.5302
-
 benzaldehyde pH and temperature not specified in the publication Homo sapiens
1.2.1.3 0.6474
-
 propanal pH and temperature not specified in the publication Homo sapiens
1.2.1.3 7.374
-
 glyceraldehyde pH and temperature not specified in the publication Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.3 cytosol
-
 Mus musculus 5829
-
1.2.1.3 mitochondrion
-
 Mus musculus 5739
-
1.2.1.3 additional information not detected in microsomes Mus musculus
-
-
1.2.1.3 nucleus
-
 Mus musculus 5634
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.3 55000
-
 MALDI-TOF mass spectrometry Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.3 Homo sapiens
-
-
-
1.2.1.3 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.3 Ni2+-Sepharose column chromatography and Superdex S200 gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.3 bladder
-
 Mus musculus
-
1.2.1.3 brain highest ALDH7A1 expression Mus musculus
-
1.2.1.3 colon
-
 Mus musculus
-
1.2.1.3 duodenum
-
 Mus musculus
-
1.2.1.3 eye
-
 Mus musculus
-
1.2.1.3 heart
-
 Mus musculus
-
1.2.1.3 ileum
-
 Mus musculus
-
1.2.1.3 jejunum
-
 Mus musculus
-
1.2.1.3 kidney highest ALDH7A1 expression, ALDH7A1 in the kidney localizes to endothelial and podocyte cell nuclei Mus musculus
-
1.2.1.3 liver highest ALDH7A1 expression Mus musculus
-
1.2.1.3 lung
-
 Mus musculus
-
1.2.1.3 pancreas highest ALDH7A1 expression Mus musculus
-
1.2.1.3 stomach
-
 Mus musculus
-
1.2.1.3 testis
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.3 2-nonenal + NAD+ + H2O
-
 Homo sapiens (E)-non-2-enoate + NADH + H+
-
 ?
1.2.1.3 alpha-aminoadipate semialdehyde + NAD+ + H2O
-
 Homo sapiens alpha-aminoadipic acid + NADH + H+
-
 ?
1.2.1.3 benzaldehyde + NAD+ + H2O low affinity for benzaldehyde Homo sapiens benzoate + NADH + H+
-
 ?
1.2.1.3 betaine aldehyde + NAD+ + H2O
-
 Homo sapiens betaine + NADH + H+
-
 ?
1.2.1.3 glyceraldehyde + NAD+ + H2O
-
 Homo sapiens glycerate + NADH + H+
-
 ?
1.2.1.3 hexanal + NAD+ + H2O high specificity Homo sapiens hexanoate + NADH + H+
-
 ?
1.2.1.3 n-nonanal + NAD+ + H2O high specificity Homo sapiens n-nonanoate + NADH + H+
-
 ?
1.2.1.3 octanal + NAD+ + H2O high specificity Homo sapiens octanoate + NADH + H+
-
 ?
1.2.1.3 propanal + NAD+ + H2O
-
 Homo sapiens propionate + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.3 aldehyde dehydrogenase 7A1
-
 Mus musculus
1.2.1.3 aldehyde dehydrogenase 7A1
-
 Homo sapiens
1.2.1.3 Aldh7a1
-
 Mus musculus
1.2.1.3 Aldh7a1
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.3 NAD+
-
 Mus musculus
1.2.1.3 NAD+
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
1.2.1.3 malfunction ALDH7A1 knockdown sensitizes human renal proximal tubular epithelial cells and CHO cells to hyperosmotic stress Homo sapiens
1.2.1.3 physiological function ALDH7A1 is involved in cellular defense against hyperosmotic stress Mus musculus
1.2.1.3 physiological function ALDH7A1 is involved in cellular defense against hyperosmotic stress Homo sapiens