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Literature summary extracted from
- Localization, purification, and functional reconstitution of the P4-ATPase Atp8a2, a phosphatidylserine flippase in photoreceptor disc membranes (2009), J. Biol. Chem., 284, 32670-32679.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.6.2.1 | additional information | no activation by phosphatidylcholine or other membrane lipids | Bos taurus | |
| 7.6.2.1 | phosphatidylethanolamine | activates to a lesser degree | Bos taurus |  |
| 7.6.2.1 | phosphatidylserine | - |
Bos taurus | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 7.6.2.1 | gene atp8a2, DNA and amino acid sequence determination and analysis, expression in HEK-293 cells | Mus musculus |
| 7.6.2.1 | gene atp8a2, DNA and amino acid sequence determination and analysis, expression in HEK-293 cells | Homo sapiens |
| 7.6.2.1 | gene atp8a2, DNA and amino acid sequence determination and analysis, expression of 1D4-tagged Atp8a2 in HEK-293 cells | Bos taurus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.6.2.1 | additional information | no inhibition by ouabain and azide | Bos taurus | |
| 7.6.2.1 | NEM | over 90% inhibition of ATPase activity at 1 mM | Bos taurus | |
| 7.6.2.1 | vanadate | over 90% inhibition of ATPase activity at 0.1 mM | Bos taurus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 7.6.2.1 | additional information | - |
additional information | kinetics, overview | Homo sapiens | |
| 7.6.2.1 | additional information | - |
additional information | Michaelis-Menten kinetics, overview | Bos taurus | |
| 7.6.2.1 | 0.704 | - |
ATP | pH 7.5, 37°C | Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 7.6.2.1 | membrane | - |
Mus musculus | 16020 | - |
| 7.6.2.1 | membrane | - |
Homo sapiens | 16020 | - |
| 7.6.2.1 | membrane | - |
Bos taurus | 16020 | - |
| 7.6.2.1 | additional information | localization study by immunofluorescence microscopy | Homo sapiens | - |
- |
| 7.6.2.1 | additional information | localization study by immunofluorescence microscopy | Bos taurus | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.6.2.1 | Mg2+ | required | Mus musculus | |
| 7.6.2.1 | Mg2+ | required | Homo sapiens | |
| 7.6.2.1 | Mg2+ | required | Bos taurus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 7.6.2.1 | 132000 | - |
x * 132000, about, recombinant enzyme, SDS-PAGE | Homo sapiens |
| 7.6.2.1 | 132000 | - |
x * 132000, recombinant enzyme, SDS-PAGE | Bos taurus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.6.2.1 | ATP + H2O + phosphatidylethanolamine/in | Bos taurus | - |
ADP + phosphate + phosphatidylethanolamine/out | - |
? | |
| 7.6.2.1 | ATP + H2O + phosphatidylserine/in | Bos taurus | - |
ADP + phosphate + phosphatidylserine/out | - |
? | |
| 7.6.2.1 | ATP + H2O + phospholipid/in | Mus musculus | - |
ADP + phosphate + phospholipid/out | - |
? | |
| 7.6.2.1 | ATP + H2O + phospholipid/in | Homo sapiens | - |
ADP + phosphate + phospholipid/out | - |
? | |
| 7.6.2.1 | additional information | Mus musculus | Atp8a2 flipps fluorescent-labeled phosphatidylserine from the inner leaflet of liposomes, equivalent to the exocytoplasmic leaflet of cell membranes, to the outer leaflet, equivalent to cytoplasmic leaflet, in an ATP-dependent manner | ? | - |
? | |
| 7.6.2.1 | additional information | Homo sapiens | Atp8a2 flipps fluorescent-labeled phosphatidylserine from the inner leaflet of liposomes, equivalent to the exocytoplasmic leaflet of cell membranes, to the outer leaflet, equivalent to cytoplasmic leaflet, in an ATP-dependent manner | ? | - |
? | |
| 7.6.2.1 | additional information | Bos taurus | Atp8a2 flipps fluorescent-labeled phosphatidylserine from the inner leaflet of liposomes, equivalent to the exocytoplasmic leaflet of cell membranes, to the outer leaflet, equivalent to cytoplasmic leaflet, in an ATP-dependent manner | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.6.2.1 | Bos taurus | C7EXK4 | gene atp8a2 | - |
| 7.6.2.1 | Homo sapiens | Q9NTI2 | gene atp8a2 | - |
| 7.6.2.1 | Mus musculus | P98200 | C57/B6 and BALB/c mice, gene atp8a2 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 7.6.2.1 | native Atp8a2 1887fold from photoreceptor outer segments by immunoaffinity chromatography | Bos taurus |
| 7.6.2.1 | recombinant enzyme from HEK-293 cells by immunoaffinity chromatography | Homo sapiens |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 7.6.2.1 | purified Atp8a2 is reconstituted into liposomes containing fluorescent-labeled phosphatidylserine to measure the ability of Atp8a2 to flip phosphatidylserine across the lipid bilayer. 30% of the reconstituted Atp8a2 is inaccessible to trypsin and correspondingly ATP | Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 7.6.2.1 | additional information | localization study by immunofluorescence microscopy | Homo sapiens | - |
| 7.6.2.1 | additional information | localization study by immunofluorescence microscopy | Bos taurus | - |
| 7.6.2.1 | photoreceptor cell | disc membranes | Mus musculus | - |
| 7.6.2.1 | photoreceptor cell | disc membranes | Homo sapiens | - |
| 7.6.2.1 | photoreceptor cell | disc membranes | Bos taurus | - |
| 7.6.2.1 | retina | outer segment disc membranes of rod and cone photoreceptor cells | Mus musculus | - |
| 7.6.2.1 | retina | outer segment disc membranes of rod and cone photoreceptor cells, high expression of atp8a2 | Homo sapiens | - |
| 7.6.2.1 | retina | outer segment disc membranes of rod and cone photoreceptor cells, high expression of atp8a2 | Bos taurus | - |
| 7.6.2.1 | testis | - |
Mus musculus | - |
| 7.6.2.1 | testis | high expression of atp8a2 | Homo sapiens | - |
| 7.6.2.1 | testis | high expression of atp8a2 | Bos taurus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 7.6.2.1 | 0.054 | - |
purified native enzyme, pH 7.5, 37°C | Bos taurus |
| 7.6.2.1 | 35 | - |
purified native enzyme reconstituted in lipid vesicles, pH 7.5, 37°C | Bos taurus |
| 7.6.2.1 | 45 | 55 | purified lipid-stimulated enzyme, pH 7.5, 37°C | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.6.2.1 | ATP + H2O + phosphatidylethanolamine/in | - |
Bos taurus | ADP + phosphate + phosphatidylethanolamine/out | - |
? | |
| 7.6.2.1 | ATP + H2O + phosphatidylethanolamine/in | the enzyme is specific for ATP and shows no activity with GTP, AMP-PNP, GDP, or ADP | Bos taurus | ADP + phosphate + phosphatidylethanolamine/out | - |
? | |
| 7.6.2.1 | ATP + H2O + phosphatidylserine/in | - |
Bos taurus | ADP + phosphate + phosphatidylserine/out | - |
? | |
| 7.6.2.1 | ATP + H2O + phosphatidylserine/in | the enzyme is specific for ATP and shows no activity with GTP, AMP-PNP, GDP, or ADP | Bos taurus | ADP + phosphate + phosphatidylserine/out | - |
? | |
| 7.6.2.1 | ATP + H2O + phospholipid/in | - |
Mus musculus | ADP + phosphate + phospholipid/out | - |
? | |
| 7.6.2.1 | ATP + H2O + phospholipid/in | - |
Homo sapiens | ADP + phosphate + phospholipid/out | - |
? | |
| 7.6.2.1 | additional information | Atp8a2 flipps fluorescent-labeled phosphatidylserine from the inner leaflet of liposomes, equivalent to the exocytoplasmic leaflet of cell membranes, to the outer leaflet, equivalent to cytoplasmic leaflet, in an ATP-dependent manner | Mus musculus | ? | - |
? | |
| 7.6.2.1 | additional information | Atp8a2 flipps fluorescent-labeled phosphatidylserine from the inner leaflet of liposomes, equivalent to the exocytoplasmic leaflet of cell membranes, to the outer leaflet, equivalent to cytoplasmic leaflet, in an ATP-dependent manner | Homo sapiens | ? | - |
? | |
| 7.6.2.1 | additional information | Atp8a2 flipps fluorescent-labeled phosphatidylserine from the inner leaflet of liposomes, equivalent to the exocytoplasmic leaflet of cell membranes, to the outer leaflet, equivalent to cytoplasmic leaflet, in an ATP-dependent manner | Bos taurus | ? | - |
? | |
| 7.6.2.1 | additional information | dithionite NBD-lipid assay for detection of flippase activity | Homo sapiens | ? | - |
? | |
| 7.6.2.1 | additional information | dithionite NBD-lipid assay for detection of flippase activity | Bos taurus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 7.6.2.1 | ? | x * 132000, about, recombinant enzyme, SDS-PAGE | Homo sapiens |
| 7.6.2.1 | ? | x * 132000, recombinant enzyme, SDS-PAGE | Bos taurus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 7.6.2.1 | Atp8a2 | - |
Mus musculus |
| 7.6.2.1 | Atp8a2 | - |
Homo sapiens |
| 7.6.2.1 | Atp8a2 | - |
Bos taurus |
| 7.6.2.1 | More | the enzyme belongs to the P4-ATPase subfamily of the of P-type ATPases | Mus musculus |
| 7.6.2.1 | More | the enzyme belongs to the P4-ATPase subfamily of the of P-type ATPases | Homo sapiens |
| 7.6.2.1 | More | the enzyme belongs to the P4-ATPase subfamily of the of P-type ATPases | Bos taurus |
| 7.6.2.1 | P4-ATPase | - |
Mus musculus |
| 7.6.2.1 | P4-ATPase | - |
Homo sapiens |
| 7.6.2.1 | P4-ATPase | - |
Bos taurus |
| 7.6.2.1 | phosphatidylserine flippase | - |
Mus musculus |
| 7.6.2.1 | phosphatidylserine flippase | - |
Homo sapiens |
| 7.6.2.1 | phosphatidylserine flippase | - |
Bos taurus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 7.6.2.1 | 23 | - |
flippase assay at | Homo sapiens |
| 7.6.2.1 | 23 | - |
flippase assay at | Bos taurus |
| 7.6.2.1 | 37 | - |
ATPase assay at | Homo sapiens |
| 7.6.2.1 | 37 | - |
ATPase assay at | Bos taurus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 7.6.2.1 | 7 | 9 | with substrate phosphatidylserine | Bos taurus |
| 7.6.2.1 | 7.5 | - |
assay at | Homo sapiens |
| 7.6.2.1 | 8 | - |
with substrate phosphatidylethanolamine | Bos taurus |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 7.6.2.1 | physiological function | P4-ATPases in general are implicated in the energy-dependent translocation of aminophospholipids across cell membranes. Atp8a2 is implicated in the generation and maintenance of phosphatidylserine asymmetry in photoreceptor disc membranes | Mus musculus |
| 7.6.2.1 | physiological function | P4-ATPases in general are implicated in the energy-dependent translocation of aminophospholipids across cell membranes. Atp8a2 is implicated in the generation and maintenance of phosphatidylserine asymmetry in photoreceptor disc membranes | Homo sapiens |
| 7.6.2.1 | physiological function | P4-ATPases in general are implicated in the energy-dependent translocation of aminophospholipids across cell membranes. Atp8a2 is implicated in the generation and maintenance of phosphatidylserine asymmetry in photoreceptor disc membranes | Bos taurus |
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