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Literature summary extracted from

  • Behm-Ansmant, I.; Grosjean, H.; Massenet, S.; Motorin, Y.; Branlant, C.
    Pseudouridylation at position 32 of mitochondrial and cytoplasmic tRNAs requires two distinct enzymes in Saccharomyces cerevisiae (2004), J. Biol. Chem., 279, 52998-53006.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.28 expression in Escherichia coli Saccharomyces cerevisiae
5.4.99.28 overexpression in Escherichia coli Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
5.4.99.28 D112A mutant does not pseudouridine32 in formation in cytoplasmic tRNAGly of the DELTArib2/pus8/DELTApus9 strain Saccharomyces cerevisiae
5.4.99.28 additional information Pus9P lacking the 23 N-terminal amino acids is inactive Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.99.28 tRNA uridine32 Saccharomyces cerevisiae Rib2/Pus8p acts specifically on cytoplasmic tRNAs, whereas Pus9p modifies the same position mostly in mitochondrial tRNAs tRNA pseudouridine32
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.28 Saccharomyces cerevisiae Q12069
-
-
5.4.99.28 Saccharomyces cerevisiae Q12362
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.28 tRNA uridine32
-
 Saccharomyces cerevisiae tRNA pseudouridine32
-
 ?
5.4.99.28 tRNA uridine32 Rib2/Pus8p acts specifically on cytoplasmic tRNAs, whereas Pus9p modifies the same position mostly in mitochondrial tRNAs Saccharomyces cerevisiae tRNA pseudouridine32
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.4.99.28 Pus9
-
 Saccharomyces cerevisiae
5.4.99.28 Rib2/Pus8p
-
 Saccharomyces cerevisiae
5.4.99.28 tRNA:Psi32-synthase
-
 Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.99.28 8
-
 assay at Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
5.4.99.28 malfunction Rib2/Pus8p acts on cytoplasmic tRNAs, whereas Pus9p modifies the same position in mitochondrial tRNAs. The DELTArib2/pus8/DELTApus9, is viable only in the presence of 15 mg/liter riboflavin in the growth medium Saccharomyces cerevisiae