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Literature summary extracted from

  • Fraser, P.D.; Misawa, N.; Linden, H.; Yamano, S.; Kobayashi, K.; Sandmann, G.
    Expression in Escherichia coli, purification, and reactivation of the recombinant Erwinia uredovora phytoene desaturase (1992), J. Biol. Chem., 267, 19891-19895.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.99.31 expression in Escherichia coli Pantoea ananatis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.99.31 NAD+
-
 Pantoea ananatis
1.3.99.31 NADP+
-
 Pantoea ananatis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.99.31 membrane
-
 Pantoea ananatis 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.99.31 56200
-
 x * 56200, SDS-PAGE Pantoea ananatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.99.31 15-cis-phytoene + 4 acceptor Pantoea ananatis overall reaction. all-trans-Lycopene is the principal desaturase product constituting 60% of the total reaction products. The desaturase intermediates all-trans-phytofluene and all-trans-zeta-carotene are also detected and constitute 27 and 12% of the total desaturase products formed all-trans-lycopene + 4 reduced acceptor
-
 ?
1.3.99.31 all-trans-neurosporene + acceptor Pantoea ananatis
-
 all-trans-lycopene + reduced acceptor
-
 ?
1.3.99.31 all-trans-phytofluene + 3 acceptor Pantoea ananatis
-
 all-trans-lycopene + 3 reduced acceptor
-
 ?
1.3.99.31 all-trans-zeta-carotene + 2 acceptor Pantoea ananatis
-
 all-trans-lycopene + 2 reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.99.31 Pantoea ananatis P21685
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.99.31
-
 Pantoea ananatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.99.31 15-cis-phytoene + 4 acceptor overall reaction. all-trans-Lycopene is the principal desaturase product constituting 60% of the total reaction products. The desaturase intermediates all-trans-phytofluene and all-trans-zeta-carotene are also detected and constitute 27 and 12% of the total desaturase products formed Pantoea ananatis all-trans-lycopene + 4 reduced acceptor
-
 ?
1.3.99.31 all-trans-neurosporene + acceptor
-
 Pantoea ananatis all-trans-lycopene + reduced acceptor
-
 ?
1.3.99.31 all-trans-phytofluene + 3 acceptor
-
 Pantoea ananatis all-trans-lycopene + 3 reduced acceptor
-
 ?
1.3.99.31 all-trans-zeta-carotene + 2 acceptor
-
 Pantoea ananatis all-trans-lycopene + 2 reduced acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.99.31 ? x * 56200, SDS-PAGE Pantoea ananatis

Synonyms

EC Number Synonyms Comment Organism
1.3.99.31 CrtI
-
 Pantoea ananatis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.99.31 8
-
 assay at Pantoea ananatis

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.99.31 FAD involved in desaturation. FAD is a dissociable moiety and not covalently bound to the enzyme Pantoea ananatis

General Information

EC Number General Information Comment Organism
1.3.99.31 physiological function the enzyme is involved in carotenoid biosynthesis Pantoea ananatis