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Literature summary extracted from

  • Raisig, A.; Bartley, G.; Scolnik, P.; Sandmann, G.
    Purification in an active state and properties of the 3-step phytoene desaturase from Rhodobacter capsulatus overexpressed in Escherichia coli (1996), J. Biochem., 119, 559-564.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.99.28 expression in Escherichia coli Rhodobacter capsulatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.99.28 Diphenylamine
-
 Rhodobacter capsulatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.99.28 0.0166
-
 all-trans-zeta-carotene pH 8.0, 30°C Rhodobacter capsulatus
1.3.99.28 0.0333
-
 15-cis-phytoene pH 8.0, 30°C Rhodobacter capsulatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.99.28 57000
-
 x * 57000, SDS-PAGE Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.99.28 15-cis-phytoene + 3 acceptor Rhodobacter capsulatus involved in carotenoid biosynthesis all-trans-neurosporene + 3 reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.99.28 Rhodobacter capsulatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.99.28
-
 Rhodobacter capsulatus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.3.99.28 15-cis-phytoene + 3 acceptor = all-trans-neurosporene + 3 reduced acceptor overall reaction Rhodobacter capsulatus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.99.28 0.00029
-
 pH 8.0, 30°C Rhodobacter capsulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.99.28 1,2-epoxyphytoene + acceptor
-
 Rhodobacter capsulatus 1,2-epoxyneurosporene + reduced acceptor
-
 ?
1.3.99.28 15-cis-phytoene + 3 acceptor involved in carotenoid biosynthesis Rhodobacter capsulatus all-trans-neurosporene + 3 reduced acceptor
-
 ?
1.3.99.28 15-cis-phytoene + 3 acceptor intermediates are phytofluene and zeta-carotene. The proportion of all-trans versus cis isomers is about 20% for phytofluene, 46% for zeta-carotene, and 72% for neurosporene Rhodobacter capsulatus all-trans-neurosporene + 3 reduced acceptor
-
 ?
1.3.99.28 all-trans-phytofluene + 2 acceptor
-
 Rhodobacter capsulatus all-trans-neurosporene + 2 reduced acceptor
-
 ?
1.3.99.28 all-trans-zeta-carotene + acceptor
-
 Rhodobacter capsulatus all-trans-neurosporene + reduced acceptor
-
 ?
1.3.99.28 additional information C30-diapophytoene is not a substrate Rhodobacter capsulatus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.99.28 ? x * 57000, SDS-PAGE Rhodobacter capsulatus

Synonyms

EC Number Synonyms Comment Organism
1.3.99.28 3-step phytoene desaturase
-
 Rhodobacter capsulatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.99.28 FAD a low basal reaction is found in the absence of all coenzymes. Upon addition of FAD the activity is stimulated 5-fold. Km-value: 0.0049 mM Rhodobacter capsulatus
1.3.99.28 additional information NAD+, NADP+ or ATP have no positive effect Rhodobacter capsulatus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.3.99.28 0.23
-
 pH 8.0, 30°C Rhodobacter capsulatus Diphenylamine

General Information

EC Number General Information Comment Organism
1.3.99.28 physiological function involved in carotenoid biosynthesis Rhodobacter capsulatus