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Literature summary extracted from

  • Thompson, J.; Hill, K.K.; Smith, T.J.; Pikis, A.
    The gene CBO0515 from Clostridium botulinum strain Hall A encodes the rare enzyme N5-(carboxyethyl) ornithine synthase, EC 1.5.1.24 (2010), J. Bacteriol., 192, 1151-1155.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.24 expressed in Escherichia coli Clostridium botulinum

Protein Variants

EC Number Protein Variants Comment Organism
1.5.1.24 D114A severe reduction (higher than 95%) in enzymatic activity Clostridium botulinum
1.5.1.24 E13A severe reduction (higher than 95%) in enzymatic activity Clostridium botulinum
1.5.1.24 H92L severe reduction (higher than 95%) in enzymatic activity Clostridium botulinum
1.5.1.24 K71R severe reduction (higher than 95%) in enzymatic activity Clostridium botulinum
1.5.1.24 R15K severe reduction (higher than 95%) in enzymatic activity Clostridium botulinum
1.5.1.24 W90S severe reduction (higher than 95%) in enzymatic activity Clostridium botulinum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.24 0.15
-
 pyruvate pH 7.5, temperature not specified in the publication, Vmax: 0.9 micromol/min/mg protein Clostridium botulinum
1.5.1.24 2.95
-
 NADPH pH 7.5, temperature not specified in the publication Clostridium botulinum
1.5.1.24 29.5
-
 L-ornithine pH 7.5, temperature not specified in the publication Clostridium botulinum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.24 35850
-
 calculated from cDNA Clostridium botulinum
1.5.1.24 36000
-
 SDS-PAGE Clostridium botulinum
1.5.1.24 125000
-
 gel filtration Clostridium botulinum

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.24 Clostridium botulinum A5HZ59 UniProt
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.24 using anion-exchange and phosphocellulose P-11 chromatography Clostridium botulinum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.24 6.6
-
 pH 7.5, temperature not specified in the publication Clostridium botulinum

Storage Stability

EC Number Storage Stability Organism
1.5.1.24 -20°C, 20% glycerol, freezing and thawing results in loss of all enzymatic activity Clostridium botulinum
1.5.1.24 -20°C, 25% (NH4)2SO4, several months, no loss in activity Clostridium botulinum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.24 alpha-N-acetylornithine + pyruvate + NADPH + H+ weak reaction rate Clostridium botulinum ? + NADP+ + H2O
-
 ?
1.5.1.24 D-lysine + pyruvate + NADPH + H+ enzyme also reacts with D-isomer Clostridium botulinum ? + NADP+ + H2O
-
 ?
1.5.1.24 D-ornithine + pyruvate + NADPH + H+ enzyme also reacts with D-isomer Clostridium botulinum N5-(L-1-carboxyethyl)-D-ornithine + NADP+ + H2O
-
 ?
1.5.1.24 L-lysine + pyruvate + NADPH + H+ weak reaction rate Clostridium botulinum ? + NADP+ + H2O
-
 ?
1.5.1.24 L-ornithine + 2-oxo-butanoic acid + NADPH + H+ weak reaction rate Clostridium botulinum ? + NADP+ + H2O
-
 ?
1.5.1.24 L-ornithine + 3-fluoropyruvic acid + NADPH + H+ weak reaction rate Clostridium botulinum ? + NADP+ + H2O
-
 ?
1.5.1.24 L-ornithine + 3-mercaptopyruvic acid + NADPH + H+ weak reaction rate Clostridium botulinum ? + NADP+ + H2O
-
 ?
1.5.1.24 L-ornithine + glyoxylic acid + NADPH + H+ weak reaction rate Clostridium botulinum ? + NADP+ + H2O
-
 ?
1.5.1.24 L-ornithine + methylglyoxal + NADPH + H+ weak reaction rate Clostridium botulinum ? + NADP+ + H2O
-
 ?
1.5.1.24 L-ornithine + oxaloacetic acid + NADPH + H+ weak reaction rate Clostridium botulinum ? + NADP+ + H2O
-
 ?
1.5.1.24 L-ornithine + pyruvate + NADPH + H+ highest reaction rate Clostridium botulinum N5-(L-1-carboxyethyl)-L-ornithine + NADP+ + H2O
-
 ?
1.5.1.24 N2-methylornithine + pyruvate + NADPH + H+ weak reaction rate Clostridium botulinum ? + NADP+ + H2O
-
 ?
1.5.1.24 N6-hydroxy-L-lysine + pyruvate + NADPH + H+ weak reaction rate Clostridium botulinum ? + NADP+ + H2O
-
 ?
1.5.1.24 O-(2-aminoethyl)-L-serine + pyruvate + NADPH + H+ weak reaction rate Clostridium botulinum ? + NADP+ + H2O
-
 ?
1.5.1.24 S-(2-aminoethyl)-L- cysteine + pyruvate + NADPH + H+ weak reaction rate Clostridium botulinum ? + NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.24 homotetramer in solution, gel filtration Clostridium botulinum

Synonyms

EC Number Synonyms Comment Organism
1.5.1.24 bCEOS
-
 Clostridium botulinum
1.5.1.24 CBO0515
-
 Clostridium botulinum
1.5.1.24 N5-(carboxyethyl) ornithine synthase
-
 Clostridium botulinum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.24 7.5
-
 assay at Clostridium botulinum