BRENDA - Enzyme Database

The Rv1712 locus from Mycobacterium tuberculosis H37Rv codes for a functional CMP kinase that preferentially phosphorylates dCMP

Thum, C.; Schneider, C.Z.; Palma, M.S.; Santos, D.S.; Basso, L.A.; J. Bacteriol. 191, 2884-2887 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.4.25
expressed in Escherichia coli BL21(DE3) cells
Mycobacterium tuberculosis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.25
0.12
-
CMP
pH and temperature not specified in the publication
Mycobacterium tuberculosis
2.7.4.25
0.165
-
dCMP
pH and temperature not specified in the publication
Mycobacterium tuberculosis
2.7.4.25
13.85
-
UMP
pH and temperature not specified in the publication
Mycobacterium tuberculosis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.4.25
21000
-
gel filtration
Mycobacterium tuberculosis
2.7.4.25
23930
-
ESI-MS
Mycobacterium tuberculosis
2.7.4.25
24060
-
calculated from amino acid sequence
Mycobacterium tuberculosis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.4.25
Mycobacterium tuberculosis
-
-
-
2.7.4.25
Mycobacterium tuberculosis H37Rv
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.4.25
anionic-exchange column chromatography, gel filtration, and anionic-exchange resin column chromatography
Mycobacterium tuberculosis
Storage Stability
EC Number
Storage Stability
Organism
2.7.4.25
-80C, no apparent loss of activity
Mycobacterium tuberculosis
2.7.4.25
4C, up to 30 days, recombinant CMK remains stable
Mycobacterium tuberculosis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.25
ATP + CMP
the enzyme preferentially phosphorylates CMP and dCMP
712276
Mycobacterium tuberculosis
ADP + CDP
-
-
-
?
2.7.4.25
ATP + CMP
the enzyme preferentially phosphorylates CMP and dCMP
712276
Mycobacterium tuberculosis H37Rv
ADP + CDP
-
-
-
?
2.7.4.25
ATP + dCMP
the enzyme preferentially phosphorylates CMP and dCMP
712276
Mycobacterium tuberculosis
ADP + dCDP
-
-
-
?
2.7.4.25
ATP + dCMP
the enzyme preferentially phosphorylates CMP and dCMP
712276
Mycobacterium tuberculosis H37Rv
ADP + dCDP
-
-
-
?
2.7.4.25
ATP + UMP
UMP is a poor substrate
712276
Mycobacterium tuberculosis
ADP + UDP
-
-
-
?
2.7.4.25
ATP + UMP
UMP is a poor substrate
712276
Mycobacterium tuberculosis H37Rv
ADP + UDP
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.4.25
monomer
1 * 21000, gel filtration
Mycobacterium tuberculosis
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.4.25
12.2
-
UMP
pH and temperature not specified in the publication
Mycobacterium tuberculosis
2.7.4.25
30
-
dCMP
pH and temperature not specified in the publication
Mycobacterium tuberculosis
2.7.4.25
52
-
CMP
pH and temperature not specified in the publication
Mycobacterium tuberculosis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.4.25
ATP
-
Mycobacterium tuberculosis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.4.25
expressed in Escherichia coli BL21(DE3) cells
Mycobacterium tuberculosis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.4.25
ATP
-
Mycobacterium tuberculosis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.25
0.12
-
CMP
pH and temperature not specified in the publication
Mycobacterium tuberculosis
2.7.4.25
0.165
-
dCMP
pH and temperature not specified in the publication
Mycobacterium tuberculosis
2.7.4.25
13.85
-
UMP
pH and temperature not specified in the publication
Mycobacterium tuberculosis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.4.25
21000
-
gel filtration
Mycobacterium tuberculosis
2.7.4.25
23930
-
ESI-MS
Mycobacterium tuberculosis
2.7.4.25
24060
-
calculated from amino acid sequence
Mycobacterium tuberculosis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.4.25
anionic-exchange column chromatography, gel filtration, and anionic-exchange resin column chromatography
Mycobacterium tuberculosis
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.4.25
-80C, no apparent loss of activity
Mycobacterium tuberculosis
2.7.4.25
4C, up to 30 days, recombinant CMK remains stable
Mycobacterium tuberculosis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.25
ATP + CMP
the enzyme preferentially phosphorylates CMP and dCMP
712276
Mycobacterium tuberculosis
ADP + CDP
-
-
-
?
2.7.4.25
ATP + CMP
the enzyme preferentially phosphorylates CMP and dCMP
712276
Mycobacterium tuberculosis H37Rv
ADP + CDP
-
-
-
?
2.7.4.25
ATP + dCMP
the enzyme preferentially phosphorylates CMP and dCMP
712276
Mycobacterium tuberculosis
ADP + dCDP
-
-
-
?
2.7.4.25
ATP + dCMP
the enzyme preferentially phosphorylates CMP and dCMP
712276
Mycobacterium tuberculosis H37Rv
ADP + dCDP
-
-
-
?
2.7.4.25
ATP + UMP
UMP is a poor substrate
712276
Mycobacterium tuberculosis
ADP + UDP
-
-
-
?
2.7.4.25
ATP + UMP
UMP is a poor substrate
712276
Mycobacterium tuberculosis H37Rv
ADP + UDP
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.4.25
monomer
1 * 21000, gel filtration
Mycobacterium tuberculosis
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.4.25
12.2
-
UMP
pH and temperature not specified in the publication
Mycobacterium tuberculosis
2.7.4.25
30
-
dCMP
pH and temperature not specified in the publication
Mycobacterium tuberculosis
2.7.4.25
52
-
CMP
pH and temperature not specified in the publication
Mycobacterium tuberculosis
General Information
EC Number
General Information
Commentary
Organism
2.7.4.25
physiological function
the physiological role of CMK is to recycle CMP to CDP, which is either rapidly phosphorylated by the unspecific nucleoside diphosphate kinase NdkA to CTP or reduced to dCDP
Mycobacterium tuberculosis
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.7.4.25
physiological function
the physiological role of CMK is to recycle CMP to CDP, which is either rapidly phosphorylated by the unspecific nucleoside diphosphate kinase NdkA to CTP or reduced to dCDP
Mycobacterium tuberculosis
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.7.4.25
0.88
-
UMP
pH and temperature not specified in the publication
Mycobacterium tuberculosis
2.7.4.25
200
-
dCMP
pH and temperature not specified in the publication
Mycobacterium tuberculosis
2.7.4.25
1100
-
CMP
pH and temperature not specified in the publication
Mycobacterium tuberculosis
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.7.4.25
0.88
-
UMP
pH and temperature not specified in the publication
Mycobacterium tuberculosis
2.7.4.25
200
-
dCMP
pH and temperature not specified in the publication
Mycobacterium tuberculosis
2.7.4.25
1100
-
CMP
pH and temperature not specified in the publication
Mycobacterium tuberculosis