Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Thum, C.; Schneider, C.Z.; Palma, M.S.; Santos, D.S.; Basso, L.A.
    The Rv1712 locus from Mycobacterium tuberculosis H37Rv codes for a functional CMP kinase that preferentially phosphorylates dCMP (2009), J. Bacteriol., 191, 2884-2887.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.25 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.25 0.12
-
 CMP pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.25 0.165
-
 dCMP pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.25 13.85
-
 UMP pH and temperature not specified in the publication Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.25 21000
-
 gel filtration Mycobacterium tuberculosis
2.7.4.25 23930
-
 ESI-MS Mycobacterium tuberculosis
2.7.4.25 24060
-
 calculated from amino acid sequence Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.25 Mycobacterium tuberculosis
-
-
-
2.7.4.25 Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.25 anionic-exchange column chromatography, gel filtration, and anionic-exchange resin column chromatography Mycobacterium tuberculosis

Storage Stability

EC Number Storage Stability Organism
2.7.4.25 -80°C, no apparent loss of activity Mycobacterium tuberculosis
2.7.4.25 4°C, up to 30 days, recombinant CMK remains stable Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.25 ATP + CMP the enzyme preferentially phosphorylates CMP and dCMP Mycobacterium tuberculosis ADP + CDP
-
 ?
2.7.4.25 ATP + CMP the enzyme preferentially phosphorylates CMP and dCMP Mycobacterium tuberculosis H37Rv ADP + CDP
-
 ?
2.7.4.25 ATP + dCMP the enzyme preferentially phosphorylates CMP and dCMP Mycobacterium tuberculosis ADP + dCDP
-
 ?
2.7.4.25 ATP + dCMP the enzyme preferentially phosphorylates CMP and dCMP Mycobacterium tuberculosis H37Rv ADP + dCDP
-
 ?
2.7.4.25 ATP + UMP UMP is a poor substrate Mycobacterium tuberculosis ADP + UDP
-
 ?
2.7.4.25 ATP + UMP UMP is a poor substrate Mycobacterium tuberculosis H37Rv ADP + UDP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.25 monomer 1 * 21000, gel filtration Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.7.4.25 CMK
-
 Mycobacterium tuberculosis
2.7.4.25 CMP kinase
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.4.25 12.2
-
 UMP pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.25 30
-
 dCMP pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.25 52
-
 CMP pH and temperature not specified in the publication Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.4.25 ATP
-
 Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
2.7.4.25 physiological function the physiological role of CMK is to recycle CMP to CDP, which is either rapidly phosphorylated by the unspecific nucleoside diphosphate kinase NdkA to CTP or reduced to dCDP Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.4.25 0.88
-
 UMP pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.25 200
-
 dCMP pH and temperature not specified in the publication Mycobacterium tuberculosis
2.7.4.25 1100
-
 CMP pH and temperature not specified in the publication Mycobacterium tuberculosis