EC Number | Cloned (Comment) | Organism |
---|---|---|
6.2.1.27 | expression of the C-terminal His6-tagged protein in Escherichia coli BL21 | Thauera aromatica |
6.2.1.27 | the gene product is expressed as a C-terminal His10-tagged protein in Escherichia coli BL21(DE3) | Azoarcus sp. |
6.2.1.37 | expression of the C-terminal His6-tagged protein in Escherichia coli BL21 | Thauera aromatica |
6.2.1.37 | the gene product is expressed as a C-terminal His10-tagged protein in Escherichia coli BL21(DE3) | Azoarcus sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.2.1.27 | 0.34 | - |
4-hydroxybenzoate | pH and temperature not specified in the publication | Thauera aromatica | |
6.2.1.37 | 0.1 | - |
3-hydroxybenzoate | pH and temperature not specified in the publication | Thauera aromatica |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.2.1.27 | 54000 | - |
x * 54000, SDS-PAGE | Azoarcus sp. |
6.2.1.27 | 57500 | - |
x * 57500, SDS-PAGE | Thauera aromatica |
6.2.1.37 | 53694 | - |
x * 53694, calculated from sequence | Azoarcus sp. |
6.2.1.37 | 54000 | - |
x * 54000, SDS-PAGE | Azoarcus sp. |
6.2.1.37 | 57500 | - |
x * 57500, SDS-PAGE | Thauera aromatica |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.2.1.27 | ATP + 4-hydroxybenzoate + CoA | Azoarcus sp. | - |
AMP + diphosphate + 4-hydroxybenzoyl-CoA | - |
? | |
6.2.1.27 | ATP + 4-hydroxybenzoate + CoA | Thauera aromatica | the bifunctional enzyme is also active with 3-hydroxybenzoate (cf. EC 6.2.1.37) | AMP + diphosphate + 4-hydroxybenzoyl-CoA | - |
? | |
6.2.1.27 | ATP + 4-hydroxybenzoate + CoA | Azoarcus sp. EbN1 | - |
AMP + diphosphate + 4-hydroxybenzoyl-CoA | - |
? | |
6.2.1.37 | ATP + 3-hydroxybenzoate + CoA | Thauera aromatica | the bifunctional enzyme is also active with 4-hydroxybenzoate (cf. EC 6.2.1.27) | AMP + diphosphate + 3-hydroxybenzoyl-CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.2.1.27 | Azoarcus sp. | Q5P0J2 | - |
- |
6.2.1.27 | Azoarcus sp. EbN1 | Q5P0J2 | - |
- |
6.2.1.27 | Thauera aromatica | Q9AJS8 | - |
- |
6.2.1.37 | Azoarcus sp. | Q5P0J2 | - |
- |
6.2.1.37 | Azoarcus sp. EbN1 | Q5P0J2 | - |
- |
6.2.1.37 | Thauera aromatica | Q9AJS8 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.2.1.27 | - |
Thauera aromatica |
6.2.1.27 | - |
Azoarcus sp. |
6.2.1.37 | - |
Thauera aromatica |
6.2.1.37 | - |
Azoarcus sp. |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
6.2.1.27 | cell suspension culture | - |
Thauera aromatica | - |
6.2.1.37 | cell suspension culture | 3-hydroxybenzoate-CoA ligase is detected not only in 3-hydroxybenzoate-grown cells but also in catechol- and protocatechuate-grown cells. The enzyme is not detected in phenol-, 4-hydroxybenzoate-, and benzoate-grown cells | Thauera aromatica | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.2.1.27 | 4.8 | - |
pH and temperature not specified in the publication | Azoarcus sp. |
6.2.1.37 | 14.3 | - |
pH and temperature not specified in the publication | Thauera aromatica |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.2.1.27 | ATP + 4-hydroxybenzoate + CoA | - |
Azoarcus sp. | AMP + diphosphate + 4-hydroxybenzoyl-CoA | - |
? | |
6.2.1.27 | ATP + 4-hydroxybenzoate + CoA | the bifunctional enzyme is also active with 3-hydroxybenzoate (cf. EC 6.2.1.37) | Thauera aromatica | AMP + diphosphate + 4-hydroxybenzoyl-CoA | - |
? | |
6.2.1.27 | ATP + 4-hydroxybenzoate + CoA | the bifunctional enzyme is also active with 3-hydroxybenzoate (at 44% of the activity with 4-hydroxybenzoate) (cf. EC 6.2.1.37) | Azoarcus sp. | AMP + diphosphate + 4-hydroxybenzoyl-CoA | - |
? | |
6.2.1.27 | ATP + 4-hydroxybenzoate + CoA | the bifunctional enzyme is also active with 3-hydroxybenzoate (cf. EC 6.2.1.37). kcat/Km for 4-hydroxybenzoate is 75% compared to the value for 3-hydroxybenzoate | Thauera aromatica | AMP + diphosphate + 4-hydroxybenzoyl-CoA | - |
? | |
6.2.1.27 | ATP + 4-hydroxybenzoate + CoA | - |
Azoarcus sp. EbN1 | AMP + diphosphate + 4-hydroxybenzoyl-CoA | - |
? | |
6.2.1.27 | ATP + 4-hydroxybenzoate + CoA | the bifunctional enzyme is also active with 3-hydroxybenzoate (at 44% of the activity with 4-hydroxybenzoate) (cf. EC 6.2.1.37) | Azoarcus sp. EbN1 | AMP + diphosphate + 4-hydroxybenzoyl-CoA | - |
? | |
6.2.1.37 | ATP + 3-hydroxybenzoate + CoA | the bifunctional enzyme is also active with 4-hydroxybenzoate (cf. EC 6.2.1.27) | Thauera aromatica | AMP + diphosphate + 3-hydroxybenzoyl-CoA | - |
? | |
6.2.1.37 | ATP + 3-hydroxybenzoate + CoA | the bifunctional enzyme is also active with 4-hydroxybenzoate (cf. EC 6.2.1.27). kcat/Km for 4-hydroxybenzoate is 75% compared to the value for 3-hydroxybenzoate | Thauera aromatica | AMP + diphosphate + 3-hydroxybenzoyl-CoA | - |
? | |
6.2.1.37 | ATP + 3-hydroxybenzoate + CoA | the bifunctional enzyme is also active with 4-hydroxybenzoate, with 2.3fold higher activity compared to 3-hydroxybenzoate (cf. EC 6.2.1.27) | Azoarcus sp. | AMP + diphosphate + 3-hydroxybenzoyl-CoA | - |
? | |
6.2.1.37 | ATP + 3-hydroxybenzoate + CoA | the bifunctional enzyme is also active with 4-hydroxybenzoate, with 2.3fold higher activity compared to 3-hydroxybenzoate (cf. EC 6.2.1.27) | Azoarcus sp. EbN1 | AMP + diphosphate + 3-hydroxybenzoyl-CoA | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.2.1.27 | ? | x * 54000, SDS-PAGE | Azoarcus sp. |
6.2.1.27 | ? | x * 57500, SDS-PAGE | Thauera aromatica |
6.2.1.37 | ? | x * 54000, SDS-PAGE | Azoarcus sp. |
6.2.1.37 | ? | x * 57500, SDS-PAGE | Thauera aromatica |
6.2.1.37 | ? | x * 53694, calculated from sequence | Azoarcus sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.2.1.27 | 3-hydroxybenzoate-CoA/4-hydroxybenzoate-CoA ligase | bifunctional enzyme EC 6.2.1.27/6.2.1.37 | Thauera aromatica |
6.2.1.27 | 4-hydroxybenzoate-CoA ligase | - |
Azoarcus sp. |
6.2.1.37 | 3-hydroxybenzoate-CoA/4-hydroxybenzoate-CoA ligase | bifunctional enzyme EC 6.2.1.27/6.2.1.37 | Thauera aromatica |
6.2.1.37 | 4-hydroxybenzoate-CoA ligase | - |
Azoarcus sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.2.1.27 | 24 | - |
4-hydroxybenzoate | pH and temperature not specified in the publication | Thauera aromatica | |
6.2.1.37 | 15 | - |
3-hydroxybenzoate | pH and temperature not specified in the publication | Thauera aromatica |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.2.1.27 | 70 | - |
4-hydroxybenzoate | pH and temperature not specified in the publication | Thauera aromatica | |
6.2.1.37 | 150 | - |
3-hydroxybenzoate | pH and temperature not specified in the publication | Thauera aromatica |