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Literature summary extracted from

  • Laempe, D.; Jahn, M.; Breese, K.; Schägger, H.; Fuchs, G.
    Anaerobic metabolism of 3-hydroxybenzoate by the denitrifying bacterium Thauera aromatica (2001), J. Bacteriol., 183, 968-979.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.27
-
 Thauera aromatica
6.2.1.37
-
 Thauera aromatica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.27 0.065
-
 4-hydroxybenzoate pH 7.8, 30°C Thauera aromatica
6.2.1.37 0.06
-
 3-hydroxybenzoate pH 7.8, 30°C Thauera aromatica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.27 60000
-
 gel filtration Thauera aromatica
6.2.1.37 60000
-
 gel filtration Thauera aromatica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.27 ATP + 4-hydroxybenzoate + CoA Thauera aromatica the enzyme is involved in the anaerobic hydroxybenzoate metabolism AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
 ?
6.2.1.37 ATP + 3-hydroxybenzoate + CoA Thauera aromatica the enzyme is involved in the anaerobic hydroxybenzoate metabolism AMP + diphosphate + 3-hydroxybenzoyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.27 Thauera aromatica Q9AJS8
-
-
6.2.1.37 Thauera aromatica Q9AJS8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.27
-
 Thauera aromatica
6.2.1.37
-
 Thauera aromatica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.27 4.12
-
 pH 7.8, 30°C Thauera aromatica
6.2.1.37 4.12
-
 pH 7.8, 30°C Thauera aromatica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.27 ATP + 4-hydroxybenzoate + CoA the enzyme is involved in the anaerobic hydroxybenzoate metabolism Thauera aromatica AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
 ?
6.2.1.27 ATP + 4-hydroxybenzoate + CoA the bifunctional enzyme is also active with 3-hydroxybenzoate (cf. EC 6.2.1.37). Activity with 4-hydroxybenzoate is 83% of the activity with 3-hydroxybenzoate Thauera aromatica AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
 ?
6.2.1.27 additional information the enzyme shows low activity towards benzoate, 4-aminobenzoate, 3-aminobenzoate, 3-fluorobenzoate, 4-fluorobenzoate, 3-chlorobenzoate, and 4-chlorobenzoate. There is no activity with 3,4-dihydroxybenzoate, 2,3-dihydroxybenzoate, and 2-hydroxybenzoate as substrates Thauera aromatica ?
-
 ?
6.2.1.37 ATP + 3-hydroxybenzoate + CoA the enzyme is involved in the anaerobic hydroxybenzoate metabolism Thauera aromatica AMP + diphosphate + 3-hydroxybenzoyl-CoA
-
 ?
6.2.1.37 ATP + 3-hydroxybenzoate + CoA the bifunctional enzyme is also active with 4-hydroxybenzoate (cf. EC 6.2.1.27). Activity with 4-hydroxybenzoate is 83% of the activity with 3-hydroxybenzoate Thauera aromatica AMP + diphosphate + 3-hydroxybenzoyl-CoA
-
 ?
6.2.1.37 additional information the enzyme shows low activity towards benzoate, 4-aminobenzoate, 3-aminobenzoate, 3-fluorobenzoate, 4-fluorobenzoate, 3-chlorobenzoate, and 4-chlorobenzoate. There is no activity with 3,4-dihydroxybenzoate, 2,3-dihydroxybenzoate, and 2-hydroxybenzoate as substrates Thauera aromatica ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.27 monomer 1 * 60000, SDS-PAGE Thauera aromatica
6.2.1.37 monomer 1 * 60000, SDS-PAGE Thauera aromatica

Synonyms

EC Number Synonyms Comment Organism
6.2.1.37 3-hydroxybenzoate-coenzyme A ligase (AMP-forming)
-
 Thauera aromatica
6.2.1.37 3-hydroxybenzoyl coenzyme A synthetase
-
 Thauera aromatica
6.2.1.37 3-hydroxybenzoyl-CoA ligase
-
 Thauera aromatica
6.2.1.37 3-hydroxybenzoyl-CoA synthetase
-
 Thauera aromatica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.27 9
-
 or above. At a pH of above 9 the coupled spectrophotometric assay becomes limited due to instability of the substrates Thauera aromatica
6.2.1.37 9
-
 or above. At a pH of above 9 the coupled spectrophotometric assay becomes limited due to instability of the substrates Thauera aromatica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.2.1.27 7 9 pH 7.0: 20% of the activity at pH 9, pH 8.0: 65% of the activity at pH 9 Thauera aromatica
6.2.1.37 7 9 pH 7.0: 20% of the activity at pH 9, pH 8.0: 65% of the activity at pH 9 Thauera aromatica

Expression

EC Number Organism Comment Expression
6.2.1.27 Thauera aromatica induction during anaerobic growth with by 3-hydroxybenzoate up
6.2.1.37 Thauera aromatica induction during anaerobic growth with by 3-hydroxybenzoate up