BRENDA - Enzyme Database

Salmonella typhimurium mutants defective in cytidine monophosphate kinase (cmk)

Beck, C.F.; Neuhard, J.; Thomassen, E.; Ingraham, J.L.; Kleker, E.; J. Bacteriol. 120, 1370-1379 (1974)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.4.25
expressed in Escherichia coli BL21(DE3) cells
Salmonella enterica subsp. enterica serovar Typhimurium
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.4.25
Salmonella enterica subsp. enterica serovar Typhimurium
-
strain JL1278
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.7.4.25
0.0023
-
enzyme from cmk mutant strain KP1300, using CMP as substrate, at pH 8.4 and 37░C
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.4.25
0.0033
-
enzyme from cmk mutant strain JL924, using CMP as substrate, at pH 8.4 and 37░C
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.4.25
0.0177
-
enzyme from cmk wild type strain JL1278, using CMP as substrate, at pH 8.4 and 37░C
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.25
ATP + CMP
-
712266
Salmonella enterica subsp. enterica serovar Typhimurium
ADP + CDP
-
-
-
?
2.7.4.25
ATP + dCMP
-
712266
Salmonella enterica subsp. enterica serovar Typhimurium
ADP + dCDP
-
-
-
?
2.7.4.25
additional information
CMP kinase from Salmonella typhimurium does not act on UMP
712266
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
-
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.4.25
ATP
-
Salmonella enterica subsp. enterica serovar Typhimurium
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.4.25
expressed in Escherichia coli BL21(DE3) cells
Salmonella enterica subsp. enterica serovar Typhimurium
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.4.25
ATP
-
Salmonella enterica subsp. enterica serovar Typhimurium
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.7.4.25
0.0023
-
enzyme from cmk mutant strain KP1300, using CMP as substrate, at pH 8.4 and 37░C
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.4.25
0.0033
-
enzyme from cmk mutant strain JL924, using CMP as substrate, at pH 8.4 and 37░C
Salmonella enterica subsp. enterica serovar Typhimurium
2.7.4.25
0.0177
-
enzyme from cmk wild type strain JL1278, using CMP as substrate, at pH 8.4 and 37░C
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.25
ATP + CMP
-
712266
Salmonella enterica subsp. enterica serovar Typhimurium
ADP + CDP
-
-
-
?
2.7.4.25
ATP + dCMP
-
712266
Salmonella enterica subsp. enterica serovar Typhimurium
ADP + dCDP
-
-
-
?
2.7.4.25
additional information
CMP kinase from Salmonella typhimurium does not act on UMP
712266
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
-