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Literature summary extracted from

  • Beck, C.F.; Neuhard, J.; Thomassen, E.; Ingraham, J.L.; Kleker, E.
    Salmonella typhimurium mutants defective in cytidine monophosphate kinase (cmk) (1974), J. Bacteriol., 120, 1370-1379.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.25 expressed in Escherichia coli BL21(DE3) cells Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.25 Salmonella enterica subsp. enterica serovar Typhimurium
-
 strain JL1278
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.25 0.0023
-
 enzyme from cmk mutant strain KP1300, using CMP as substrate, at pH 8.4 and 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.7.4.25 0.0033
-
 enzyme from cmk mutant strain JL924, using CMP as substrate, at pH 8.4 and 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.7.4.25 0.0177
-
 enzyme from cmk wild type strain JL1278, using CMP as substrate, at pH 8.4 and 37°C Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.25 ATP + CMP
-
 Salmonella enterica subsp. enterica serovar Typhimurium ADP + CDP
-
 ?
2.7.4.25 ATP + dCMP
-
 Salmonella enterica subsp. enterica serovar Typhimurium ADP + dCDP
-
 ?
2.7.4.25 additional information CMP kinase from Salmonella typhimurium does not act on UMP Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.4.25 ATP:CMP phosphotransferase
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.4.25 CMK
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.4.25 CMP kinase
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.4.25 cytidine 5’-monophosphate kinase
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.4.25 ATP
-
 Salmonella enterica subsp. enterica serovar Typhimurium