BRENDA - Enzyme Database show

Kinetic, structural, and EPR studies reveal that aldehyde oxidoreductase from Desulfovibrio gigas does not need a sulfido ligand for catalysis and give evidence for a direct Mo-C interaction in a biological system

Santos-Silva, T.; Ferroni, F.; Thapper, A.; Marangon, J.; Gonzalez, P.J.; Rizzi, A.C.; Moura, I.; Moura, J.J.; Romao, M.J.; Brondino, C.D.; J. Am. Chem. Soc. 131, 7990-7998 (2009)

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization
Organism
1.2.99.7
sitting drop vapor diffusion method, using 30% (v/v) 2-propanol, 0.2 M magnesium chloride, and 0.2 M HEPES buffer (pH 7.6)
Desulfovibrio gigas
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.99.7
arsenite
irreversible inhibitor, complete inhibition at 0.024 mM
Desulfovibrio gigas
1.2.99.7
cyanide
competitive reversible inhibitor, 30.9% inhibition at 50 mM
Desulfovibrio gigas
1.2.99.7
ethylene glycol
mixed-type reversible inhibitor, 21.9% inhibition at 1 mM
Desulfovibrio gigas
1.2.99.7
glycerol
competitive reversible inhibitor, 52% inhibition at 1 mM
Desulfovibrio gigas
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.99.7
0.0097
-
benzaldehyde
in 50 mM Tris-HCl buffer (pH 7.6), at 37°C
Desulfovibrio gigas
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.2.99.7
Molybdenum
contains molybdenum
Desulfovibrio gigas
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.99.7
Desulfovibrio gigas
Q46509
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.99.7
-
Desulfovibrio gigas
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.99.7
benzaldehyde + H2O + 2,6-dichlorophenolindophenol
-
712240
Desulfovibrio gigas
benzoate + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.2.99.7
1.12
-
benzaldehyde
in 50 mM Tris-HCl buffer (pH 7.6), at 37°C
Desulfovibrio gigas
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
1.2.99.7
sitting drop vapor diffusion method, using 30% (v/v) 2-propanol, 0.2 M magnesium chloride, and 0.2 M HEPES buffer (pH 7.6)
Desulfovibrio gigas
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.99.7
arsenite
irreversible inhibitor, complete inhibition at 0.024 mM
Desulfovibrio gigas
1.2.99.7
cyanide
competitive reversible inhibitor, 30.9% inhibition at 50 mM
Desulfovibrio gigas
1.2.99.7
ethylene glycol
mixed-type reversible inhibitor, 21.9% inhibition at 1 mM
Desulfovibrio gigas
1.2.99.7
glycerol
competitive reversible inhibitor, 52% inhibition at 1 mM
Desulfovibrio gigas
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.99.7
0.0097
-
benzaldehyde
in 50 mM Tris-HCl buffer (pH 7.6), at 37°C
Desulfovibrio gigas
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.2.99.7
Molybdenum
contains molybdenum
Desulfovibrio gigas
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.99.7
-
Desulfovibrio gigas
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.99.7
benzaldehyde + H2O + 2,6-dichlorophenolindophenol
-
712240
Desulfovibrio gigas
benzoate + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.2.99.7
1.12
-
benzaldehyde
in 50 mM Tris-HCl buffer (pH 7.6), at 37°C
Desulfovibrio gigas