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Literature summary extracted from
- Cuticle laccase of the silkworm, Bombyx mori: purification, gene identification and presence of its inactive precursor in the cuticle (2009), Insect Biochem. Mol. Biol., 39, 254-262.
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.10.3.2 | Cu2+ | multi-copper enzyme | Bombyx mori |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.10.3.2 | 70000 | - |
2 * 70000, SDS-PAGE | Bombyx mori |
| 1.10.3.2 | 84280 | - |
2 * 84280, mature enzyme, calculated from amino acid sequence | Bombyx mori |
| 1.10.3.2 | 154000 | - |
gel filtration | Bombyx mori |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 1.10.3.2 | additional information | 1% (v/v) Triton X-100 and 8 M urea have little or no influence on the strength of cuticle laccase activity | Bombyx mori |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.10.3.2 | Bombyx mori | - |
Kinsyu x Showa | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.10.3.2 | ammonium sulfate precipitation, Q-Sepharose column chromatography, HiTrap Q column chromatography, Mono Q column chromatography, and Superdex-200 gel filtration | Bombyx mori |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.10.3.2 | cuticle | pupal cuticle | Bombyx mori | - |
| 1.10.3.2 | epidermis | - |
Bombyx mori | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.10.3.2 | dopamine + O2 | - |
Bombyx mori | ? | - |
? | |
| 1.10.3.2 | methyl hydroquinone + O2 | - |
Bombyx mori | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.10.3.2 | homodimer | 2 * 70000, SDS-PAGE | Bombyx mori |
| 1.10.3.2 | homodimer | 2 * 84280, mature enzyme, calculated from amino acid sequence | Bombyx mori |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.10.3.2 | benzenediol:O2 oxidoreductase | - |
Bombyx mori |
| 1.10.3.2 | Lac2 | - |
Bombyx mori |
| 1.10.3.2 | laccase2 | - |
Bombyx mori |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 1.10.3.2 | Bombyx mori | laccase2 is highly expressed in the epidermis prior to ecdysis | up |
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