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Literature summary extracted from

  • Stibingerova, A.; Velvarska, H.; Kynclova, K.; Marounkova, B.; Spundova, M.; Entlicher, G.
    Lipoamide dehydrogenase and diaphorase catalyzed conversion of some NO donors to NO and reduction of NO scavenger 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (PTIO) (2009), Gen. Physiol. Biophys., 28, 384-390.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.4 diphenyleneiodonium chloride
-
 Bos taurus
1.8.1.4 diphenyleneiodonium chloride
-
 Sus scrofa
1.8.1.4 additional information LAD is not inhibited by resorufin ethyl ether, phenidone, Nomega-nitro-L-arginine methyl ester hydrochloride, proadiphen hydrochloride, and miconazole nitrate Bos taurus
1.8.1.4 additional information LAD is not inhibited by resorufin ethyl ether, phenidone, Nomega-nitro-L-arginine methyl ester hydrochloride, proadiphen hydrochloride, and miconazole nitrate Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.4 0.12
-
 S-nitrosoglutathione in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
1.8.1.4 0.42
-
 S-nitroso-N-acetylpenicillamine in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
1.8.1.4 0.44
-
 S-nitrosoglutathione in the presence of superoxide dismutase, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
1.8.1.4 0.46
-
 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Bos taurus
1.8.1.4 0.84
-
 sodium nitroprusside in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
1.8.1.4 0.9
-
 glycerol trinitrate in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
1.8.1.4 0.96
-
 acetaldoxime in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
1.8.1.4 1.42
-
 glycerol trinitrate in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Bos taurus
1.8.1.4 1.45
-
 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
1.8.1.4 2.26
-
 sodium nitroprusside in the presence of superoxide dismutase, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
1.8.1.4 2.27
-
 glycerol trinitrate in the presence of superoxide dismutase, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
1.8.1.4 2.73
-
 formaldoxime in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
1.8.1.4 3.24
-
 Hydroxylamine hydrochloride in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
1.8.1.4 5.48
-
 S-nitroso-N-acetylpenicillamine in the presence of superoxide dismutase, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
1.8.1.4 15.83
-
 formaldoxime in the presence of superoxide dismutase, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Bos taurus
1.8.1.4 16.93
-
 glycerol trinitrate in the presence of superoxide dismutase, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Bos taurus
1.8.1.4 17.18
-
 formaldoxime in the presence of superoxide dismutase, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
1.8.1.4 17.82
-
 formaldoxime in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.4 Bos taurus
-
-
-
1.8.1.4 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.4 heart
-
 Sus scrofa
-
1.8.1.4 intestine
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.4 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide + NADH
-
 Sus scrofa ?
-
 ?
1.8.1.4 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide + NADH
-
 Bos taurus ?
-
 ?
1.8.1.4 acetaldoxime + NADH
-
 Sus scrofa ?
-
 ?
1.8.1.4 acetaldoxime + NADH
-
 Bos taurus ?
-
 ?
1.8.1.4 dihydrolipoamide + NAD+
-
 Sus scrofa lipoamide + NADH
-
 ?
1.8.1.4 dihydrolipoamide + NAD+
-
 Bos taurus lipoamide + NADH
-
 ?
1.8.1.4 formaldoxime + NADH
-
 Sus scrofa ?
-
 ?
1.8.1.4 formaldoxime + NADH
-
 Bos taurus ?
-
 ?
1.8.1.4 glycerol trinitrate + NADH
-
 Sus scrofa ?
-
 ?
1.8.1.4 glycerol trinitrate + NADH
-
 Bos taurus ?
-
 ?
1.8.1.4 hydroxylamine hydrochloride + NADH
-
 Sus scrofa ?
-
 ?
1.8.1.4 hydroxylamine hydrochloride + NADH
-
 Bos taurus ?
-
 ?
1.8.1.4 additional information LAD is unable to convert formamidoxim, acetone oxime, acetohydroxamic acid, and Nomega-hydroxy-L-arginine Sus scrofa ?
-
 ?
1.8.1.4 additional information LAD is unable to convert formamidoxim, acetone oxime, acetohydroxamic acid, and Nomega-hydroxy-L-arginine Bos taurus ?
-
 ?
1.8.1.4 S-nitroso-N-acetylpenicillamine + NADH
-
 Sus scrofa ?
-
 ?
1.8.1.4 S-nitroso-N-acetylpenicillamine + NADH
-
 Bos taurus ?
-
 ?
1.8.1.4 S-nitrosoglutathione + NADH
-
 Sus scrofa ?
-
 ?
1.8.1.4 S-nitrosoglutathione + NADH
-
 Bos taurus ?
-
 ?
1.8.1.4 sodium nitroprusside + NADH
-
 Sus scrofa ?
-
 ?
1.8.1.4 sodium nitroprusside + NADH
-
 Bos taurus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.4 LAD
-
 Sus scrofa
1.8.1.4 LAD
-
 Bos taurus
1.8.1.4 lipoamide dehydrogenase
-
 Sus scrofa
1.8.1.4 lipoamide dehydrogenase
-
 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.4 FAD
-
 Sus scrofa
1.8.1.4 FAD
-
 Bos taurus
1.8.1.4 NADH
-
 Sus scrofa
1.8.1.4 NADH
-
 Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.8.1.4 0.29
-
 diphenyleneiodonium chloride using acetaldoxime as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
1.8.1.4 0.31
-
 diphenyleneiodonium chloride using sodium nitroprusside as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
1.8.1.4 0.35
-
 diphenyleneiodonium chloride using S-nitrosoglutathione as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
1.8.1.4 0.45
-
 diphenyleneiodonium chloride using hydroxylamine hydrochloride as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
1.8.1.4 0.56
-
 diphenyleneiodonium chloride using formaldoxime as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
1.8.1.4 0.6
-
 diphenyleneiodonium chloride using glycerol trinitrate as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa
1.8.1.4 0.8
-
 diphenyleneiodonium chloride using S-nitroso-N-acetylpenicillamine as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.8.1.4 0.59
-
 using hydroxylamine hydrochloride as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa diphenyleneiodonium chloride
1.8.1.4 0.68
-
 using sodium nitroprusside as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa diphenyleneiodonium chloride
1.8.1.4 1.75
-
 using S-nitroso-N-acetylpenicillamine as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa diphenyleneiodonium chloride
1.8.1.4 1.81
-
 using S-nitrosoglutathione as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa diphenyleneiodonium chloride
1.8.1.4 1.93
-
 using glycerol trinitrate as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa diphenyleneiodonium chloride
1.8.1.4 2.61
-
 using formaldoxime as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa diphenyleneiodonium chloride
1.8.1.4 3.31
-
 using acetaldoxime as substrate, in 50 mM Tris-HCl buffer (pH 7.6), at 37°C Sus scrofa diphenyleneiodonium chloride