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Literature summary extracted from

  • Nakade, K.; Nakagawa, Y.; Yano, A.; Sato, T.; Sakamoto, Y.
    Characterization of an extracellular laccase, PbLac1, purified from Polyporus brumalis (2010), Fungal Biol., 114, 609-618.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.2
-
Polyporus brumalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 4-coumaric acid 32.5% residual activity at 1 mM Polyporus brumalis
1.10.3.2 Ca2+ 89.8% residual activity at 1 mM Polyporus brumalis
1.10.3.2 Cd2+ 88.0% residual activity at 1 mM Polyporus brumalis
1.10.3.2 Co2+ 75.3% residual activity at 01 mM Polyporus brumalis
1.10.3.2 dithiothreitol completely inhibited by 1.0 mM dithiothreitol Polyporus brumalis
1.10.3.2 EDTA 50% residual activity at 10 mM Polyporus brumalis
1.10.3.2 K+ 65.1% residual activity at 10 mM Polyporus brumalis
1.10.3.2 kojic acid 27.7% residual activity at 1 mM Polyporus brumalis
1.10.3.2 L-cysteine completely inhibited by 1.0 mM L-cysteine Polyporus brumalis
1.10.3.2 Mg2+ 68.8% residual activity at 10 mM Polyporus brumalis
1.10.3.2 Mn2+ 82.3% residual activity at 10 mM Polyporus brumalis
1.10.3.2 Na+ 56.6% residual activity at 10 mM Polyporus brumalis
1.10.3.2 NaN3 8.9% residual activity at 1 mM Polyporus brumalis
1.10.3.2 Sn2+ 4.6% residual activity at 1 mM Polyporus brumalis
1.10.3.2 tropolone 9.7% residual activity at 1 mM Polyporus brumalis
1.10.3.2 Zn2+ 65.7% residual activity at 1 mM Polyporus brumalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.02
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) in McIlvaine buffer (pH 4.0), at 30°C Polyporus brumalis
1.10.3.2 0.07
-
2,6-dimethoxyphenol in McIlvaine buffer (pH 4.0), at 30°C Polyporus brumalis
1.10.3.2 0.12
-
ferulic acid in McIlvaine buffer (pH 5.0), at 30°C Polyporus brumalis
1.10.3.2 0.27
-
4-phenylenediamine in McIlvaine buffer (pH 5.0), at 30°C Polyporus brumalis
1.10.3.2 0.56
-
pyrogallol in McIlvaine buffer (pH 4.0), at 30°C Polyporus brumalis
1.10.3.2 0.66
-
3,4-dihydroxy L-phenylalanine in McIlvaine buffer (pH 4.0), at 30°C Polyporus brumalis
1.10.3.2 8.06
-
catechol in McIlvaine buffer (pH 4.0), at 30°C Polyporus brumalis
1.10.3.2 10.7
-
guaiacol in McIlvaine buffer (pH 4.0), at 30°C Polyporus brumalis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Cu2+ 138% activity at 10 mM Polyporus brumalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 63400
-
estimated from SDS-PAGE Polyporus brumalis

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Polyporus brumalis A3F8Z8
-
-
1.10.3.2 Polyporus brumalis ibrc05015 A3F8Z8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 ammonium sulfate precipitation and Sepharose Q column chromatography Polyporus brumalis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 mycelium
-
Polyporus brumalis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
Polyporus brumalis ?
-
?
1.10.3.2 2,6-dimethoxyphenol + O2
-
Polyporus brumalis ?
-
?
1.10.3.2 3,4-dihydroxy L-phenylalanine + O2
-
Polyporus brumalis ?
-
?
1.10.3.2 4-phenylenediamine + O2
-
Polyporus brumalis ?
-
?
1.10.3.2 catechol + O2
-
Polyporus brumalis ?
-
?
1.10.3.2 catechol + O2
-
Polyporus brumalis ibrc05015 ?
-
?
1.10.3.2 ferulic acid + O2
-
Polyporus brumalis ?
-
?
1.10.3.2 ferulic acid + O2
-
Polyporus brumalis ibrc05015 ?
-
?
1.10.3.2 guaiacol + O2
-
Polyporus brumalis ?
-
?
1.10.3.2 additional information Lac1 decolorizes 0.02% (v/v) Remazol Brilliant Blue R and Bromophenol Blue more than 90% in 100 min. Lac1 completely decolorizes 0.002% (v/v) in 100 min. Reactive Orange 16 and Poly R-478 are not decolorized by Lac1 alone. In addition, Lac1 decolorized a wide range of substrates, such as the carcinogen, Poly R-478, in the presence of violuric acid as mediator Polyporus brumalis ?
-
?
1.10.3.2 additional information Lac1 does not oxidize L-tyrosine Polyporus brumalis ?
-
?
1.10.3.2 additional information Lac1 decolorizes 0.02% (v/v) Remazol Brilliant Blue R and Bromophenol Blue more than 90% in 100 min. Lac1 completely decolorizes 0.002% (v/v) in 100 min. Reactive Orange 16 and Poly R-478 are not decolorized by Lac1 alone. In addition, Lac1 decolorized a wide range of substrates, such as the carcinogen, Poly R-478, in the presence of violuric acid as mediator Polyporus brumalis ibrc05015 ?
-
?
1.10.3.2 additional information Lac1 does not oxidize L-tyrosine Polyporus brumalis ibrc05015 ?
-
?
1.10.3.2 pyrogallol + O2
-
Polyporus brumalis ?
-
?
1.10.3.2 pyrogallol + O2
-
Polyporus brumalis ibrc05015 ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.10.3.2 LAC1
-
Polyporus brumalis
1.10.3.2 p-benzenediol:dioxygen oxidoreductase
-
Polyporus brumalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 40
-
at pH. 4.0 Polyporus brumalis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.2 20 60
-
Polyporus brumalis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 50 80 the Lac activity of Lac1 remains 80% after incubation at 50°C for 30 min. However, incubated at 60°C, the remaining activity declines in less than 80%. The enzyme is inactive at 70-80°C Polyporus brumalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 4
-
-
Polyporus brumalis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.2 3 6
-
Polyporus brumalis