EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.4.11 | expressed in Escherichia coli BL21(DE3) cells | Trypanosoma cruzi |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.4.11 | 0.013 | - |
tryparedoxin I | recombinant enzyme. isoform MSRA, at 30°C and pH 7.5 | Trypanosoma cruzi | |
1.8.4.11 | 0.033 | - |
tryparedoxin I | recombinant enzyme. isoform MSR10, at 30°C and pH 7.5 | Trypanosoma cruzi | |
1.8.4.11 | 0.069 | - |
L-methionine (S)-sulfoxide | recombinant enzyme. isoform MSRA, at 30°C and pH 7.5 | Trypanosoma cruzi | |
1.8.4.11 | 0.723 | - |
L-methionine (S)-sulfoxide | recombinant enzyme. isoform MSR10, at 30°C and pH 7.5 | Trypanosoma cruzi |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.8.4.11 | cytoplasm | - |
Trypanosoma cruzi | 5737 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.8.4.11 | 20000 | - |
deduced from amino acid sequence | Trypanosoma cruzi |
1.8.4.11 | 23000 | - |
His-tagged enzyme isoform MSRA, SDS-PAGE | Trypanosoma cruzi |
1.8.4.11 | 24000 | - |
His-tagged enzyme isoform MSR10, SDS-PAGE | Trypanosoma cruzi |
1.8.4.11 | 30000 | - |
His-tagged enzyme isoform MSR180, SDS-PAGE | Trypanosoma cruzi |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.4.11 | Trypanosoma cruzi | - |
- |
- |
1.8.4.11 | Trypanosoma cruzi CL Brener | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.4.11 | Co2+-IDA-Sepharose column chromatography | Trypanosoma cruzi |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.8.4.11 | epimastigote | - |
Trypanosoma cruzi | - |
EC Number | Storage Stability | Organism |
---|---|---|
1.8.4.11 | -80°C, 20 mM Tris-HCl pH 7.5, 1 mM EDTA, and 4% (v/v) glycerol, at least 12 months, the purified recombinant enzyme remains stable | Trypanosoma cruzi |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.4.11 | L-methionine (S)-sulfoxide + tryparedoxin I | L-methionine (S)-sulfoxide is the specific substrate | Trypanosoma cruzi | L-methionine + tryparedoxin I disulfide + H2O | - |
? | |
1.8.4.11 | L-methionine (S)-sulfoxide + tryparedoxin I | L-methionine (S)-sulfoxide is the specific substrate | Trypanosoma cruzi CL Brener | L-methionine + tryparedoxin I disulfide + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.8.4.11 | monomer | 1 * 20000, deduced from amino acid sequence | Trypanosoma cruzi |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.4.11 | methionine sulfoxide reductase A | - |
Trypanosoma cruzi |
1.8.4.11 | MSR10 | isoform | Trypanosoma cruzi |
1.8.4.11 | MSR180 | isoform | Trypanosoma cruzi |
1.8.4.11 | MsrA | isoform | Trypanosoma cruzi |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.4.11 | 0.32 | - |
L-methionine (S)-sulfoxide | recombinant enzyme. isoform MSRA, at 30°C and pH 7.5 | Trypanosoma cruzi | |
1.8.4.11 | 0.32 | - |
tryparedoxin I | recombinant enzyme. isoform MSRA, at 30°C and pH 7.5 | Trypanosoma cruzi | |
1.8.4.11 | 0.52 | - |
L-methionine (S)-sulfoxide | recombinant enzyme. isoform MSR10, at 30°C and pH 7.5 | Trypanosoma cruzi | |
1.8.4.11 | 0.52 | - |
tryparedoxin I | recombinant enzyme. isoform MSR10, at 30°C and pH 7.5 | Trypanosoma cruzi |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.4.11 | thioredoxin | - |
Trypanosoma cruzi |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.8.4.11 | physiological function | overexpression of isoform MSR10 in Trypanosoma cruzi confers resistance to oxidative stress, as transfected cells exhibit around 2fold more tolerance to exogenous H2O2 | Trypanosoma cruzi |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.4.11 | 0.0086 | - |
L-methionine (S)-sulfoxide | recombinant enzyme. isoform MSR180, at 30°C and pH 7.5 | Trypanosoma cruzi | |
1.8.4.11 | 4.6 | - |
L-methionine (S)-sulfoxide | recombinant enzyme. isoform MSRA, at 30°C and pH 7.5 | Trypanosoma cruzi | |
1.8.4.11 | 7.2 | - |
L-methionine (S)-sulfoxide | recombinant enzyme. isoform MSR10, at 30°C and pH 7.5 | Trypanosoma cruzi | |
1.8.4.11 | 24 | - |
tryparedoxin I | recombinant enzyme. isoform MSRA, at 30°C and pH 7.5 | Trypanosoma cruzi | |
1.8.4.11 | 77 | - |
tryparedoxin I | recombinant enzyme. isoform MSR180, at 30°C and pH 7.5 | Trypanosoma cruzi | |
1.8.4.11 | 180 | - |
tryparedoxin I | recombinant enzyme. isoform MSR10, at 30°C and pH 7.5 | Trypanosoma cruzi |