EC Number | Cloned (Comment) | Organism |
---|---|---|
3.6.1.23 | - |
Homo sapiens |
3.6.1.23 | - |
Mycobacterium tuberculosis |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.6.1.23 | mutant enzymes D28N and T138STOP | Mycobacterium tuberculosis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.6.1.23 | D28N/H145W | the Asp/Asn mutation within Motif I results in approximately 50fold decrease of steady-state rate and significantly weakens the interaction of the protein with alpha,beta-imido-dUTP (15fold) whereas dUMP binding is only slightly affected | Mycobacterium tuberculosis |
3.6.1.23 | D49N/F158W | the Asp/Asn mutation within motif I results in approximately 1000fold decrease of steady-state rate and significantly weakens the interaction of the protein with alpha,beta-imido-dUTP (4fold) whereas dUMP binding is only slightly affected | Homo sapiens |
3.6.1.23 | F158W | the mutation has no significant effect on the catalytic properties of dUTPase | Homo sapiens |
3.6.1.23 | H145W | the mutation has no significant effect on the catalytic properties of dUTPase | Mycobacterium tuberculosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.23 | alpha,beta-imido-dUTP | - |
Mycobacterium tuberculosis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.23 | Mg2+ | required for full activity, In the absence of divalent cations, dUTPase retains about 50% of the catalytic rate observed in the presence of Mg2+ | Homo sapiens | |
3.6.1.23 | Mg2+ | required for full activity, In the absence of divalent cations, dUTPase retains about 50% of the catalytic rate observed in the presence of Mg2+ | Mycobacterium tuberculosis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.23 | Homo sapiens | - |
- |
- |
3.6.1.23 | Mycobacterium tuberculosis | P9WNS5 | - |
- |
3.6.1.23 | Mycobacterium tuberculosis H37Rv | P9WNS5 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.6.1.23 | - |
Homo sapiens |
3.6.1.23 | - |
Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.23 | dUTP + H2O | - |
Homo sapiens | dUMP + diphosphate | - |
? | |
3.6.1.23 | dUTP + H2O | - |
Mycobacterium tuberculosis | dUMP + diphosphate | - |
? | |
3.6.1.23 | dUTP + H2O | - |
Mycobacterium tuberculosis H37Rv | dUMP + diphosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.6.1.23 | homotrimer | - |
Homo sapiens |
3.6.1.23 | homotrimer | - |
Mycobacterium tuberculosis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.6.1.23 | dUTPase | - |
Homo sapiens |
3.6.1.23 | dUTPase | - |
Mycobacterium tuberculosis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.23 | 0.007 | - |
dUTP | mutant enzyme D49N/F158W, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C | Homo sapiens | |
3.6.1.23 | 0.024 | - |
dUTP | mutant enzyme D28N/H145W, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C | Mycobacterium tuberculosis | |
3.6.1.23 | 1.2 | - |
dUTP | mutant enzyme H145W, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C | Mycobacterium tuberculosis | |
3.6.1.23 | 6.3 | - |
dUTP | mutant enzyme F158W, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C | Homo sapiens |