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Literature summary extracted from

  • Otagiri, M.; Ui, S.; Takusagawa, Y.; Ohtsuki, T.; Kurisu, G.; Kusunoki, M.
    Structural basis for chiral substrate recognition by two 2,3-butanediol dehydrogenases (2010), FEBS Lett., 584, 219-223.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.76 in complex with NAD+ and 2-mercaptoethanol, to 2.0 A resolution. The L-BDH subunit is composed of seven beta-strands, six alpha-helices and two short a helices, aFG1 and aFG2, which form a small lobe on top of the core domain. The conserved active site residues Asn112, Ser141, Tyr154 and Lys158 are located near the NAD+ molecule Corynebacterium glutamicum

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.B20 N146F 11% of wild-type specific activity Corynebacterium glutamicum
1.1.1.B20 Q140I trace activity below 0.1 U/mg Corynebacterium glutamicum
1.1.1.B20 Q140I/N146F poor activity Corynebacterium glutamicum
1.1.1.B20 Q140I/N146F/W190H trace activity below 0.1 U/mg with substrate meso-butanediol, 2.9 U/mg with substrate (2S,3S)-2,3-butanediol Corynebacterium glutamicum
1.1.1.76 F148N trace activity below 0.1 U/mg Corynebacterium glutamicum
1.1.1.76 I142Q trace activity below 0.1 U/mg Corynebacterium glutamicum
1.1.1.76 I142Q/F148N trace activity below 0.1 U/mg Corynebacterium glutamicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.76 2-mercaptoethanol competitive. In crystal structure, the mercaptoethanol molecule is located in the catalytic cleft formed by residues Ser141, Ileu142, Ala143, Phe148, Tyr154, Pro184, Gly185, Ileu186, Met191, Trp192 and Ile195. These residues may also be involved in substrate recognition and in stereospecific redox reactions Corynebacterium glutamicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.B20 2
-
 meso-2,3-butanediol pH 8.0, 37°C Corynebacterium glutamicum

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.B20 Corynebacterium glutamicum
-
-
-
1.1.1.76 Corynebacterium glutamicum Q9ZNN8
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.B20 1.1
-
 mutant N146F, substrate (2S,3S)-2,3-butanediol, pH 8.0, 37°C Corynebacterium glutamicum
1.1.1.B20 2.9
-
 mutant Q140I/N146F/W190H, substrate (2S,3S)-2,3-butanediol, pH 8.0, 37°C Corynebacterium glutamicum
1.1.1.B20 3.1
-
 mutant Q140I/N146F, substrate (2,3S)-2,3-butanediol, pH 8.0, 37°C Corynebacterium glutamicum
1.1.1.B20 4.5
-
 mutant Q140I/N146F, substrate meso-2,3-butanediol, pH 8.0, 37°C Corynebacterium glutamicum
1.1.1.B20 5.5
-
 wild-type, substrate (2S,3S)-2,3-butanediol, pH 8.0, 37°C Corynebacterium glutamicum
1.1.1.B20 19.4
-
 mutant N146F, substrate meso-2,3-butanediol, pH 8.0, 37°C Corynebacterium glutamicum
1.1.1.B20 177
-
 wild-type, substrate meso-2,3-butanediol, pH 8.0, 37°C Corynebacterium glutamicum
1.1.1.76 0.1
-
 substrate meso-2,3-butanediol, pH 8.0, 37°C Corynebacterium glutamicum
1.1.1.76 4.3
-
 substrate (2S,3S)-2,3-butanediol, pH 8.0, 37°C Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.B20 (2S,3S)-2,3-butanediol + NAD+ (2S,3S)-2,3-butanediol is a poor substrate Corynebacterium glutamicum (3S)-acetoin + NADH
-
 ?
1.1.1.B20 meso-2,3-butanediol + NAD+
-
 Corynebacterium glutamicum acetoin + NADH
-
 ?
1.1.1.76 (2S,3S)-2,3-butanediol + NAD+
-
 Corynebacterium glutamicum (3S)-acetoin + NADH + H+
-
 ?
1.1.1.76 meso-2,3-butanediol + NAD+ poor substrate Corynebacterium glutamicum acetoin + NADH
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.B20 meso-BD
-
 Corynebacterium glutamicum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.76 4.3
-
 2-mercaptoethanol pH 8.0, 37°C Corynebacterium glutamicum