Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sutter, M.; Striebel, F.; Damberger, F.F.; Allain, F.H.; Weber-Ban, E.
    A distinct structural region of the prokaryotic ubiquitin-like protein (Pup) is recognized by the N-terminal domain of the proteasomal ATPase Mpa (2009), FEBS Lett., 583, 3151-3157.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.6.1.5 characterization of the structural determinants of Pup (mybacterial ubiquitin-like protein) and its interaction witrh Mpa. The N-terminal coiled-coil domain of Mpa makes extensive contacts along the central region (residues 21-58) of Pup leaving its N-terminal uncontrained and available for other functional interactions Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
5.6.1.5 Mycobacterium tuberculosis P9WQN5
-
-
5.6.1.5 Mycobacterium tuberculosis H37Rv P9WQN5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.1.5 Mpa fragments are purified as N-terminal fusions with His6-tagged maltose-binding protein followed by a TEV cleavage site, producing the folowing fragments: Mpa-wt (residues 1-609), Mpa-CC (residues 1-98), Mpa-ID (99-216), and Mpa-CC-ID (1-216) Mycobacterium tuberculosis

Subunits

EC Number Subunits Comment Organism
5.6.1.5 hexamer Mpa is a monomer of 67000 Da that assembles into a hexameric ring. Each monomer consists of three main regions, an N-terminal coiled-coil domain (CC, residues 1-98), an interdomain (ID, residues 99-216) and an ATPase domain of the AAA-type (residues 217-609). The N-terminal coiled-coil domain of Mpa specifically interacts with Pub-decorated beads, indicating that it mediates the binding to pupylated substrates Mycobacterium tuberculosis
5.6.1.5 monomer Mpa is a monomer of 67000 Da that assembles into a hexameric ring. Each monomer consists of three main regions, an N-terminal coiled-coil domain (CC, residues 1-98), an interdomain (ID, residues 99-216) and an ATPase domain of the AAA-type (residues 217-609). The N-terminal coiled-coil domain of Mpa specifically interacts with Pub-decorated beads, indicating that it mediates the binding to pupylated substrates Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
5.6.1.5 ARC
-
 Mycobacterium tuberculosis
5.6.1.5 mpA
-
 Mycobacterium tuberculosis
5.6.1.5 proteasomal ATPase Mpa
-
 Mycobacterium tuberculosis