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Literature summary extracted from

  • Spadiut, O.; Tan, T.C.; Pisanelli, I.; Haltrich, D.; Divne, C.
    Importance of the gating segment in the substrate-recognition loop of pyranose 2-oxidase (2010), FEBS J., 277, 2892-2909.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.10 expressed in Escherichia coli BL21 Star (DE3) cells Trametes ochracea

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.3.10 mutant enzymes are crystallized by the hanging drop vapor diffusion method, using 10% (w/v) monomethyl ether poly(ethlene glycol) 2000 , 0.1 M MES (pH 5.2), 50 mM MgCl2 Trametes ochracea

Protein Variants

EC Number Protein Variants Comment Organism
1.1.3.10 F454A/S455A/Y456A the mutant shows decreased catalytic efficiency for D-glucose/O2 compared to the wild type enzyme Trametes ochracea
1.1.3.10 F454A/Y456A the mutant shows decreased catalytic efficiency for D-glucose/O2 compared to the wild type enzyme Trametes ochracea
1.1.3.10 F454N the apparent kcat value with D-glucose and O2 (fixed at a concentration of 0.256 mM, air saturation) as substrates decreases dramatically compared to the wild type enzyme Trametes ochracea
1.1.3.10 F454N the mutant shows decreased catalytic efficiency for D-glucose/O2 compared to the wild type enzyme Trametes ochracea
1.1.3.10 F454P the mutant shows strongly decreased catalytic efficiency for D-glucose/O2 compared to the wild type enzyme Trametes ochracea
1.1.3.10 H450Q the turnover number for the electron donor/acceptor substrate pair D-galactose/O2 is comparable to that of the wild type enzyme Trametes ochracea
1.1.3.10 additional information the deletion mutant DELTA454-456 shows 6fold lower kcat (D-galactose/O2), and 0.8fold elevated Km (D-galactose) value compared to the wild type enzyme Trametes ochracea
1.1.3.10 Y456W the turnover number for the electron donor/acceptor substrate pair D-galactose/O2 is comparable to that of the wild type enzyme Trametes ochracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.10 0.0052
-
 1,4-benzoquinone mutant enzyme F454N, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.0071
-
 1,4-benzoquinone mutant enzyme F454P, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.0089
-
 1,4-benzoquinone mutant enzyme F454A/S455A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.01
-
 1,4-benzoquinone mutant enzyme Y456W, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.013
-
 1,4-benzoquinone mutant enzyme H450Q, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.015
-
 ferrocenium hexafluorophosphate mutant enzyme F454A/S455A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.016
-
 ferrocenium hexafluorophosphate mutant enzyme F454A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.023
-
 ferrocenium hexafluorophosphate mutant enzyme F454P, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.025
-
 ferrocenium hexafluorophosphate mutant enzyme F454N, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.027
-
 1,4-benzoquinone wild type enzyme, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.029
-
 1,4-benzoquinone mutant enzyme F454A/S455A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.029
-
 1,4-benzoquinone mutant enzyme F454A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.041
-
 ferrocenium hexafluorophosphate mutant enzyme Y456W, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.049
-
 ferrocenium hexafluorophosphate mutant enzyme H450Q, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.052
-
 1,4-benzoquinone mutant enzyme F454N, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.072
-
 1,4-benzoquinone mutant enzyme F454P, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.072
-
 1,4-benzoquinone mutant enzyme Y456W, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.078
-
 1,4-benzoquinone mutant enzyme F454A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.1
-
 ferrocenium hexafluorophosphate wild type enzyme, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.14
-
 ferrocenium hexafluorophosphate mutant enzyme F454P, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.14
-
 1,4-benzoquinone wild type enzyme, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.15
-
 ferrocenium hexafluorophosphate mutant enzyme F454A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.24
-
 1,4-benzoquinone mutant enzyme H450Q, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.24
-
 ferrocenium hexafluorophosphate mutant enzyme Y456W, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.28
-
 ferrocenium hexafluorophosphate mutant enzyme F454A/S455A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.35
-
 ferrocenium hexafluorophosphate mutant enzyme F454N, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.38
-
 ferrocenium hexafluorophosphate mutant enzyme H450Q, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.4
-
 ferrocenium hexafluorophosphate wild type enzyme, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.76
-
 D-glucose wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 1.5
-
 D-glucose mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 1.5
-
 D-glucose mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 1.7
-
 D-glucose mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 1.8
-
 D-glucose mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 2 3 D-melibiose mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 2.1
-
 D-glucose mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 2.5
-
 D-glucose mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 6.1
-
 D-galactose wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 10
-
 D-galactose mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 13
-
 D-galactose mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 13
-
 D-galactose mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 26
-
 D-galactose mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 29
-
 D-galactose mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 34
-
 D-galactose mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 50
-
 D-melibiose mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 240
-
 D-melibiose mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 260
-
 D-melibiose mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 350
-
 D-melibiose mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 390
-
 D-melibiose mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 1500
-
 D-melibiose wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.10 270000
-
 gel filtration Trametes ochracea

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.10 Trametes ochracea Q7ZA32 synonym Trametes ochracea
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.10 Ni2+-immobilized metal affinity column chromatography Trametes ochracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.10 2-deoxy-2-fluoro-D-glucose + O2 slow substrate Trametes ochracea 2-deoxy-3-dehydro-D-glucose + H2O2
-
 ?
1.1.3.10 beta-D-galactose + O2 poor substrate Trametes ochracea 2-dehydro-D-galactose + H2O2
-
 ?
1.1.3.10 D-galactose + 1,4-benzoquinone
-
 Trametes ochracea 2-dehydro-D-galactose + hydroquinone
-
 ?
1.1.3.10 D-galactose + ferrocenium hexafluorophosphate
-
 Trametes ochracea 2-dehydro-D-galactose + ferrocene
-
 ?
1.1.3.10 D-glucose + 1,4-benzoquinone 1,4-benzoquinone is a physiologically relevant alternative electron acceptor in the oxidative half-reaction Trametes ochracea 2-dehydro-D-glucose + hydroquinone
-
 ?
1.1.3.10 D-glucose + ferrocenium hexafluorophosphate
-
 Trametes ochracea 2-dehydro-D-glucose + ferrocene
-
 ?
1.1.3.10 D-glucose + O2 the wild type enzyme displays a clear preference for D-glucose over D-galactose Trametes ochracea 2-dehydro-D-glucose + H2O2
-
 ?
1.1.3.10 D-melibiose + O2
-
 Trametes ochracea ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.10 homotetramer 2 * 000, SDS-PAGE Trametes ochracea

Synonyms

EC Number Synonyms Comment Organism
1.1.3.10 P2O
-
 Trametes ochracea
1.1.3.10 pyranose 2-Oxidase
-
 Trametes ochracea
1.1.3.10 pyranose:oxygen 2-oxidoreductase
-
 Trametes ochracea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.10 60 70 the half life of the wild type enzyme at 60°C and 70°C is 12 min and 0.07 min, respectively. The melting temperature of the wild type enzyme is at 63.5°C Trametes ochracea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.10 0.14
-
 D-galactose mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.19
-
 D-melibiose mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.2
-
 D-glucose mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.29
-
 D-melibiose mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.3
-
 D-galactose mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.64
-
 D-galactose mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.99
-
 D-glucose mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 1.2
-
 1,4-benzoquinone mutant enzyme F454A/S455A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 1.2
-
 D-galactose mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 1.3
-
 D-melibiose mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 1.5
-
 D-galactose mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 1.7
-
 D-galactose wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 2
-
 1,4-benzoquinone mutant enzyme F454P, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 2
-
 D-galactose mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 2.7
-
 1,4-benzoquinone mutant enzyme F454N, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 2.7
-
 D-melibiose mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 2.9
-
 1,4-benzoquinone mutant enzyme F454A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 2.9
-
 ferrocenium hexafluorophosphate mutant enzyme F454A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 3
-
 1,4-benzoquinone mutant enzyme H450Q, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 3.3
-
 D-melibiose mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 3.3
-
 1,4-benzoquinone mutant enzyme Y456W, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 3.8
-
 1,4-benzoquinone wild type enzyme, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 4.4
-
 D-melibiose mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 4.5
-
 ferrocenium hexafluorophosphate mutant enzyme F454P, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 5.4
-
 ferrocenium hexafluorophosphate mutant enzyme F454A/S455A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 6.6
-
 ferrocenium hexafluorophosphate wild type enzyme, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 7.1
-
 D-glucose mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 7.3
-
 ferrocenium hexafluorophosphate mutant enzyme F454N, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 7.3
-
 ferrocenium hexafluorophosphate mutant enzyme Y456W, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 7.6
-
 D-melibiose wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 12
-
 D-glucose mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 15
-
 1,4-benzoquinone mutant enzyme F454A/S455A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 17
-
 ferrocenium hexafluorophosphate mutant enzyme H450Q, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 17
-
 D-glucose mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 26
-
 D-glucose mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 30
-
 1,4-benzoquinone mutant enzyme F454P, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 33
-
 D-glucose wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 61
-
 1,4-benzoquinone mutant enzyme F454A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 67
-
 ferrocenium hexafluorophosphate mutant enzyme F454P, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 74
-
 ferrocenium hexafluorophosphate mutant enzyme F454A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 110
-
 ferrocenium hexafluorophosphate mutant enzyme F454A/S455A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 130
-
 1,4-benzoquinone mutant enzyme F454N, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 160
-
 1,4-benzoquinone wild type enzyme, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 210
-
 ferrocenium hexafluorophosphate wild type enzyme, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 220
-
 1,4-benzoquinone mutant enzyme H450Q, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 220
-
 1,4-benzoquinone mutant enzyme Y456W, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 400
-
 ferrocenium hexafluorophosphate mutant enzyme Y456W, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 420
-
 ferrocenium hexafluorophosphate mutant enzyme F454N, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 470
-
 ferrocenium hexafluorophosphate mutant enzyme H450Q, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.10 FAD
-
 Trametes ochracea

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.3.10 0.0037
-
 D-melibiose mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.005
-
 D-melibiose wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.0058
-
 D-melibiose mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.008
-
 D-melibiose mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.0084
-
 D-melibiose mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.011
-
 D-galactose mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.0113
-
 D-melibiose mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.0166
-
 D-melibiose mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.024
-
 D-galactose mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.046
-
 D-galactose mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.053
-
 D-galactose mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.059
-
 D-galactose mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.062
-
 D-galactose mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.094
-
 D-glucose mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.27
-
 D-galactose wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 0.54
-
 D-glucose mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 4.7
-
 D-glucose mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 7
-
 D-glucose mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 8.2
-
 D-glucose mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 15
-
 D-glucose mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 37
-
 1,4-benzoquinone mutant enzyme F454A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 43
-
 D-glucose wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 63
-
 ferrocenium hexafluorophosphate wild type enzyme, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 130
-
 1,4-benzoquinone mutant enzyme F454A/S455A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 140
-
 1,4-benzoquinone wild type enzyme, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 180
-
 ferrocenium hexafluorophosphate mutant enzyme F454A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 180
-
 ferrocenium hexafluorophosphate mutant enzyme Y456W, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 200
-
 ferrocenium hexafluorophosphate mutant enzyme F454P, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 230
-
 1,4-benzoquinone mutant enzyme H450Q, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 280
-
 1,4-benzoquinone mutant enzyme F454P, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 290
-
 ferrocenium hexafluorophosphate mutant enzyme F454N, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 330
-
 1,4-benzoquinone mutant enzyme Y456W, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 340
-
 ferrocenium hexafluorophosphate mutant enzyme H450Q, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 360
-
 ferrocenium hexafluorophosphate mutant enzyme F454A/S455A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 370
-
 ferrocenium hexafluorophosphate mutant enzyme F454A/S455A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 420
-
 1,4-benzoquinone mutant enzyme F454P, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 480
-
 ferrocenium hexafluorophosphate mutant enzyme F454P, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 500
-
 ferrocenium hexafluorophosphate mutant enzyme F454A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 510
-
 ferrocenium hexafluorophosphate wild type enzyme, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 520
-
 1,4-benzoquinone mutant enzyme F454A/S455A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 520
-
 1,4-benzoquinone mutant enzyme F454N, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 940
-
 1,4-benzoquinone mutant enzyme H450Q, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 1200
-
 1,4-benzoquinone wild type enzyme, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 1210
-
 ferrocenium hexafluorophosphate mutant enzyme F454N, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 1250
-
 ferrocenium hexafluorophosphate mutant enzyme H450Q, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 1640
-
 ferrocenium hexafluorophosphate mutant enzyme Y456W, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 2100
-
 1,4-benzoquinone mutant enzyme F454A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 2600
-
 1,4-benzoquinone mutant enzyme F454N, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea
1.1.3.10 3000
-
 1,4-benzoquinone mutant enzyme Y456W, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C Trametes ochracea