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Literature summary extracted from

  • Wieser, M.; Fujii, N.; Yoshida, T.; Nagasawa T.
    Carbon dioxide fixation by reversible pyrrole-2-carboxylate decarboxylase from Bacillus megaterium PYR2910 (1998), Eur. J. Biochem., 257, 495-499.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.93 2-mercaptoethanol reducing agents in optimal concentrations of 20 mM or above are a prerequisite for high CO2 fixation turnovers, with dithiothreitol enhancing the carboxylation 16.2fold compared with a control without reducing agent, followed by ascorbate (15.5fold), Na2S2O5 (13.6fold) and 2-mercaptoethanol (7.2fold) Priestia megaterium
4.1.1.93 ascorbate reducing agents in optimal concentrations of 20 mM or above are a prerequisite for high CO2 fixation turnovers, with dithiothreitol enhancing the carboxylation 16.2fold compared with a control without reducing agent, followed by ascorbate (15.5fold), Na2S2O5 (13.6fold) and 2-mercaptoethanol (7.2fold) Priestia megaterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.93 Pyrrole substrate inhibition of carboxylation above 300 mM Priestia megaterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.93 61
-
 Pyrrole pH 5.5, 20°C Priestia megaterium
4.1.1.93 560
-
 HCO3- pH 5.5, 20°C Priestia megaterium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.93 Na2S2O5 reducing agents in optimal concentrations of 20 mM or above are a prerequisite for high CO2 fixation turnovers, with dithiothreitol enhancing the carboxylation 16.2fold compared with a control without reducing agent, followed by ascorbate (15.5fold), Na2S2O5 (13.6fold) and 2-mercaptoethanol (7.2fold) Priestia megaterium

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.93 Priestia megaterium
-
-
-
4.1.1.93 Priestia megaterium PYR2910
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.1.1.93 pyrrole-2-carboxylate + H2O = pyrrole + HCO3- the enzyme catalyses both the carboxylation and decarboxylation reactions. However, while bicarbonate is the preferred substrate for the carboxylation reaction, decarboxylation produces carbon dioxide Priestia megaterium
a
4.1.1.93 pyrrole-2-carboxylate = pyrrole + CO2 the enzyme catalyses both the carboxylation and decarboxylation reactions. However, while bicarbonate is the preferred substrate for the carboxylation reaction, decarboxylation produces carbon dioxide Priestia megaterium
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.93 additional information no carboxylation of thiophene, furan, indole, 1-methylpyrrole, 1-(2-cyanethyl)pyrrole, pyrrolidine, pyrazine, pyridine, pyridine-4-carboxamide, imidazole, n-butylimidazole, 1,2,3-triazole, 1,2,4-triazole, 1,3,5-triazine and thiazole Priestia megaterium ?
-
 ?
4.1.1.93 additional information no carboxylation of thiophene, furan, indole, 1-methylpyrrole, 1-(2-cyanethyl)pyrrole, pyrrolidine, pyrazine, pyridine, pyridine-4-carboxamide, imidazole, n-butylimidazole, 1,2,3-triazole, 1,2,4-triazole, 1,3,5-triazine and thiazole Priestia megaterium PYR2910 ?
-
 ?
4.1.1.93 pyrrole + HCO3- the enzyme catalyses both the carboxylation and decarboxylation reactions. However, while bicarbonate is the preferred substrate for the carboxylation reaction, decarboxylation produces carbon dioxide Priestia megaterium pyrrole-2-carboxylate + H2O
-
 ?
4.1.1.93 pyrrole + HCO3- the enzyme catalyses both the carboxylation and decarboxylation reactions. However, while bicarbonate is the preferred substrate for the carboxylation reaction, decarboxylation produces carbon dioxide Priestia megaterium PYR2910 pyrrole-2-carboxylate + H2O
-
 ?
4.1.1.93 pyrrole-2-carboxylate the enzyme catalyses both the carboxylation and decarboxylation reactions. However, while bicarbonate is the preferred substrate for the carboxylation reaction, decarboxylation produces carbon dioxide Priestia megaterium pyrrole + CO2
-
 ?
4.1.1.93 pyrrole-2-carboxylate the enzyme catalyses both the carboxylation and decarboxylation reactions. However, while bicarbonate is the preferred substrate for the carboxylation reaction, decarboxylation produces carbon dioxide Priestia megaterium PYR2910 pyrrole + CO2
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.93 20
-
 assay at, carboxylase reaction Priestia megaterium
4.1.1.93 30
-
 assay at, decarboxylase reaction Priestia megaterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.93 5.5
-
 assay at, carboxylase reaction Priestia megaterium
4.1.1.93 7
-
 assay at, decarboxylase reaction Priestia megaterium

General Information

EC Number General Information Comment Organism
4.1.1.93 physiological function despite a strong reverse reaction, it is assumed that reversible pyrrole-2-carboxylate decarboxylase takes part in the catabolism of pyrrole-2-carboxylate due to a slow decrease of the decarboxylation product pyrrole under resting cells conditions and the non-physiologically high Km-value for HCO23, which is substantially above the concentrations of HCO23 that probably occur in vivo Priestia megaterium