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Literature summary extracted from

  • Tsuda, T.; Toyoshima, C.
    Nucleotide recognition by CopA, a Cu+-transporting P-type ATPase (2009), EMBO J., 28, 1782-1791.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.2.2.8 expressed in Escherichia coli Top10 cells Archaeoglobus fulgidus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
7.2.2.8 with bound adenosine 5'-[beta, gamma-methylene]triphosphate or ADP and Mg2+, vapor diffusion method, using 5% (w/v) PEG 6000, 1.25-1.5M NaCl and 100 mM MES, pH 5.5-5.75, at 10°C Archaeoglobus fulgidus

Protein Variants

EC Number Protein Variants Comment Organism
7.2.2.8 H462Q the mutation reduces the affinity for adenosine 5'-[beta, gamma-methylene]triphosphate about 20fold Archaeoglobus fulgidus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.2.2.8 Mg2+ required Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.2.2.8 ATP + H2O + Cu+[side 1] Archaeoglobus fulgidus
-
 ADP + phosphate + Cu+[side 2]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.2.2.8 Archaeoglobus fulgidus O29777
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.2.2.8 Ni Sepharose 6 column chromatography and Superdex 75 gel filtration Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.2.2.8 ATP + H2O + Cu+[side 1]
-
 Archaeoglobus fulgidus ADP + phosphate + Cu+[side 2]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.2.2.8 CopA
-
 Archaeoglobus fulgidus
7.2.2.8 Cu+-transporting P-type ATPase
-
 Archaeoglobus fulgidus