EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.3.1 | expressed in Escherichia coli M15 cells | Oryctolagus cuniculus |
1.2.3.1 | expressed in Escherichia coli M15 cells | Macaca fascicularis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.2.3.1 | A1023Y | the mutant shows increased Km towards (+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine compared to the wild type enzyme | Macaca fascicularis |
1.2.3.1 | A1081V | the mutant completely loses the high cinchonidine oxidation activity of the wild type enzyme | Oryctolagus cuniculus |
1.2.3.1 | A1083T | the mutant shows increased Km towards (+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine compared to the wild type enzyme | Macaca fascicularis |
1.2.3.1 | A1083T/V1085A | the mutant shows increased Km towards (+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine compared to the wild type enzyme | Macaca fascicularis |
1.2.3.1 | I1032V | the mutant shows increased Km towards (+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine compared to the wild type enzyme | Macaca fascicularis |
1.2.3.1 | K1004Q | the mutant shows increased Km towards (+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine compared to the wild type enzyme | Macaca fascicularis |
1.2.3.1 | K1004Q/K1005R | the mutant shows increased Km towards (+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine compared to the wild type enzyme | Macaca fascicularis |
1.2.3.1 | K1004Q/K1005R/M1009I/V1010I | the mutant shows increased Km towards (+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine compared to the wild type enzyme | Macaca fascicularis |
1.2.3.1 | K1004Q/K1005R/M1009I/V1010I/R1021V/A1023Y | the mutant shows increased Km towards (+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine compared to the wild type enzyme | Macaca fascicularis |
1.2.3.1 | K1004Q/K1005R/M1009I/V1010I/R1021V/A1023Y/I1032V | the mutant shows increased Km towards (+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine compared to the wild type enzyme | Macaca fascicularis |
1.2.3.1 | K1004Q/K1005R/M1009I/V1010I/R1021V/A1023Y/I1032V/G1064K/I1067M | the mutant shows increased Km towards (+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine compared to the wild type enzyme | Macaca fascicularis |
1.2.3.1 | M1009I | the mutant shows increased Km towards (+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine compared to the wild type enzyme | Macaca fascicularis |
1.2.3.1 | V1085A | the mutant acquires high cinchonidine oxidation activity | Macaca fascicularis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.3.1 | 0.0074 | - |
cinchonidine | wild type enzyme, in 80 mM phosphate buffer, pH 7.4, at 37°C | Oryctolagus cuniculus | |
1.2.3.1 | 0.0109 | - |
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine | wild type enzyme, in 80 mM phosphate buffer, pH 7.4, at 37°C | Macaca fascicularis | |
1.2.3.1 | 0.012 | - |
cinchonidine | mutant enzyme V1085A, in 80 mM phosphate buffer, pH 7.4, at 37°C | Macaca fascicularis | |
1.2.3.1 | 0.0157 | - |
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine | mutant enzyme K1004Q/K1005R/M1009I/V1010I/R1021V/A1023Y/I1032V/G1064K/I1067M, in 80 mM phosphate buffer, pH 7.4, at 37°C | Macaca fascicularis | |
1.2.3.1 | 0.0244 | - |
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine | mutant enzyme K1004Q/K1005R/M1009I/V1010I/R1021V/A1023Y/I1032V, in 80 mM phosphate buffer, pH 7.4, at 37°C | Macaca fascicularis | |
1.2.3.1 | 0.0264 | - |
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine | mutant enzyme A1023Y, in 80 mM phosphate buffer, pH 7.4, at 37°C | Macaca fascicularis | |
1.2.3.1 | 0.027 | - |
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine | mutant enzyme M1009I, in 80 mM phosphate buffer, pH 7.4, at 37°C | Macaca fascicularis | |
1.2.3.1 | 0.0334 | - |
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine | mutant enzyme I1032V, in 80 mM phosphate buffer, pH 7.4, at 37°C | Macaca fascicularis | |
1.2.3.1 | 0.0352 | - |
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine | mutant enzyme A1081V, in 80 mM phosphate buffer, pH 7.4, at 37°C | Oryctolagus cuniculus | |
1.2.3.1 | 0.036 | - |
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine | mutant enzyme K1004Q/K1005R, in 80 mM phosphate buffer, pH 7.4, at 37°C | Macaca fascicularis | |
1.2.3.1 | 0.0366 | - |
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine | mutant enzyme K1004Q, in 80 mM phosphate buffer, pH 7.4, at 37°C | Macaca fascicularis | |
1.2.3.1 | 0.0521 | - |
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine | mutant enzyme K1004Q/K1005R/M1009I/V1010I, in 80 mM phosphate buffer, pH 7.4, at 37°C | Macaca fascicularis | |
1.2.3.1 | 0.0965 | - |
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine | mutant enzyme K1004Q/K1005R/M1009I/V1010I/R1021V/A1023Y, in 80 mM phosphate buffer, pH 7.4, at 37°C | Macaca fascicularis | |
1.2.3.1 | 0.128 | - |
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine | mutant enzyme A1083T/V1085A, in 80 mM phosphate buffer, pH 7.4, at 37°C | Macaca fascicularis | |
1.2.3.1 | 0.131 | - |
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine | mutant enzyme V1085A, in 80 mM phosphate buffer, pH 7.4, at 37°C | Macaca fascicularis | |
1.2.3.1 | 0.147 | - |
(+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine | wild type enzyme, in 80 mM phosphate buffer, pH 7.4, at 37°C | Oryctolagus cuniculus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.3.1 | Macaca fascicularis | - |
- |
- |
1.2.3.1 | Oryctolagus cuniculus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.3.1 | Ni affinity column chromatography | Oryctolagus cuniculus |
1.2.3.1 | Ni affinity column chromatography | Macaca fascicularis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.3.1 | (+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine + H2O + O2 | i.e. (S)-RS-8359 | Macaca fascicularis | ? | - |
? | |
1.2.3.1 | (+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine + H2O + O2 | i.e. (S)-RS-8359 | Oryctolagus cuniculus | ? | - |
? | |
1.2.3.1 | cinchonidine + H2O + O2 | rabbit AOX1 has an extremely high Vmax value toward cinchonidine | Oryctolagus cuniculus | ? | - |
? | |
1.2.3.1 | cinchonidine + H2O + O2 | wild type monkey AOX1 is not able to oxidize chinchonidine, but mutant enzyme V1085A does | Macaca fascicularis | ? | - |
? | |
1.2.3.1 | zonisamide + H2O + O2 | - |
Oryctolagus cuniculus | ? | - |
? | |
1.2.3.1 | zonisamide + H2O + O2 | - |
Macaca fascicularis | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.3.1 | aldehyde oxidase 1 | - |
Oryctolagus cuniculus |
1.2.3.1 | aldehyde oxidase 1 | - |
Macaca fascicularis |
1.2.3.1 | AOX1 | - |
Oryctolagus cuniculus |
1.2.3.1 | AOX1 | - |
Macaca fascicularis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.3.1 | FAD | - |
Oryctolagus cuniculus | |
1.2.3.1 | FAD | - |
Macaca fascicularis |