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Literature summary extracted from

  • Stripp, S.T.; Happe, T.
    How algae produce hydrogen--news from the photosynthetic hydrogenase (2009), Dalton Trans., 2009, 9960-9969.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.7.2 Fe2+ the protein contains iron but no nickel, the active site cofactor is a unique [Fe-S] compound commonly referred to as H-cluster Chlamydomonas reinhardtii
1.12.7.2 Fe2+ the protein contains iron but no nickel, the active site cofactor is a unique [Fe-S] compound commonly referred to as H-cluster Tetradesmus obliquus
1.12.7.2 Fe2+ the protein contains iron but no nickel, the active site cofactor is a unique [Fe-S] compound commonly referred to as H-cluster Clostridium pasteurianum
1.12.7.2 Fe2+ the protein contains iron but no nickel, the active site cofactor is a unique [Fe-S] compound commonly referred to as H-cluster [Chlorella] fusca
1.12.7.2 Fe2+ the protein contains iron but no nickel, the active site cofactor is a unique [Fe-S] compound commonly referred to as H-cluster Megasphaera elsdenii
1.12.7.2 Fe2+ the protein contains iron but no nickel, the active site cofactor is a unique [Fe-S] compound commonly referred to as H-cluster Desulfovibrio desulfuricans
1.12.7.2 Fe2+ the protein contains iron but no nickel, the active site cofactor is a unique [Fe-S] compound commonly referred to as H-cluster Clostridium acetobutylicum
1.12.7.2 Fe2+ the protein contains iron but no nickel, the active site cofactor is a unique [Fe-S] compound commonly referred to as H-cluster Chlamydomonas moewusii
1.12.7.2 Fe2+ the protein contains iron but no nickel, the active site cofactor is a unique [Fe-S] compound commonly referred to as H-cluster Chlorococcum submarinum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.7.2 14000
-
 1 * 46100 + 1 * 14000, calculated from amino acid sequence Desulfovibrio desulfuricans
1.12.7.2 44600
-
 calculated from amino acid sequence Tetradesmus obliquus
1.12.7.2 45100
-
 calculated from amino acid sequence [Chlorella] fusca
1.12.7.2 45300
-
 calculated from amino acid sequence Chlorococcum submarinum
1.12.7.2 45400
-
 calculated from amino acid sequence Chlamydomonas moewusii
1.12.7.2 46100
-
 1 * 46100 + 1 * 14000, calculated from amino acid sequence Desulfovibrio desulfuricans
1.12.7.2 47300
-
 isozyme HydA2, calculated from amino acid sequence Chlamydomonas reinhardtii
1.12.7.2 47500
-
 isozyme HydA1, calculated from amino acid sequence Chlamydomonas reinhardtii
1.12.7.2 48000
-
 isozyme HydA1, SDS-PAGE Chlamydomonas reinhardtii
1.12.7.2 53600
-
 calculated from amino acid sequence Megasphaera elsdenii
1.12.7.2 63800
-
 calculated from amino acid sequence Clostridium pasteurianum
1.12.7.2 64300
-
 calculated from amino acid sequence Clostridium acetobutylicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.7.2 H+ + reduced ferredoxin Chlamydomonas reinhardtii hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin H2 + oxidized ferredoxin
-
 ?
1.12.7.2 H+ + reduced ferredoxin Tetradesmus obliquus hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin H2 + oxidized ferredoxin
-
 ?
1.12.7.2 H+ + reduced ferredoxin Clostridium pasteurianum hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin H2 + oxidized ferredoxin
-
 ?
1.12.7.2 H+ + reduced ferredoxin [Chlorella] fusca hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin H2 + oxidized ferredoxin
-
 ?
1.12.7.2 H+ + reduced ferredoxin Megasphaera elsdenii hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin H2 + oxidized ferredoxin
-
 ?
1.12.7.2 H+ + reduced ferredoxin Desulfovibrio desulfuricans hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin H2 + oxidized ferredoxin
-
 ?
1.12.7.2 H+ + reduced ferredoxin Clostridium acetobutylicum hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin H2 + oxidized ferredoxin
-
 ?
1.12.7.2 H+ + reduced ferredoxin Chlamydomonas moewusii hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin H2 + oxidized ferredoxin
-
 ?
1.12.7.2 H+ + reduced ferredoxin Chlorococcum submarinum hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin H2 + oxidized ferredoxin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.12.7.2 Chlamydomonas moewusii
-
-
-
1.12.7.2 Chlamydomonas reinhardtii
-
-
-
1.12.7.2 Chlorococcum submarinum
-
-
-
1.12.7.2 Clostridium acetobutylicum
-
-
-
1.12.7.2 Clostridium pasteurianum
-
-
-
1.12.7.2 Desulfovibrio desulfuricans
-
-
-
1.12.7.2 Megasphaera elsdenii
-
-
-
1.12.7.2 Tetradesmus obliquus
-
-
-
1.12.7.2 [Chlorella] fusca
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.7.2 affinity column chromatography with immobilised ferredoxin Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.7.2 H+ + reduced ferredoxin hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin Chlamydomonas reinhardtii H2 + oxidized ferredoxin
-
 ?
1.12.7.2 H+ + reduced ferredoxin hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin Tetradesmus obliquus H2 + oxidized ferredoxin
-
 ?
1.12.7.2 H+ + reduced ferredoxin hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin Clostridium pasteurianum H2 + oxidized ferredoxin
-
 ?
1.12.7.2 H+ + reduced ferredoxin hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin [Chlorella] fusca H2 + oxidized ferredoxin
-
 ?
1.12.7.2 H+ + reduced ferredoxin hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin Megasphaera elsdenii H2 + oxidized ferredoxin
-
 ?
1.12.7.2 H+ + reduced ferredoxin hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin Desulfovibrio desulfuricans H2 + oxidized ferredoxin
-
 ?
1.12.7.2 H+ + reduced ferredoxin hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin Clostridium acetobutylicum H2 + oxidized ferredoxin
-
 ?
1.12.7.2 H+ + reduced ferredoxin hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin Chlamydomonas moewusii H2 + oxidized ferredoxin
-
 ?
1.12.7.2 H+ + reduced ferredoxin hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin Chlorococcum submarinum H2 + oxidized ferredoxin
-
 ?
1.12.7.2 H+ + reduced methyl viologen
-
 Chlamydomonas reinhardtii H2 + oxidized methyl viologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.7.2 heterodimer 1 * 46100 + 1 * 14000, calculated from amino acid sequence Desulfovibrio desulfuricans

Synonyms

EC Number Synonyms Comment Organism
1.12.7.2 CpI
-
 Clostridium pasteurianum
1.12.7.2 DDH
-
 Desulfovibrio desulfuricans
1.12.7.2 hydA
-
 Tetradesmus obliquus
1.12.7.2 hydA
-
 [Chlorella] fusca
1.12.7.2 hydA
-
 Megasphaera elsdenii
1.12.7.2 hydA
-
 Clostridium acetobutylicum
1.12.7.2 hydA
-
 Chlorococcum submarinum
1.12.7.2 HydA1
-
 Chlamydomonas moewusii
1.12.7.2 HydA1 isozyme Chlamydomonas reinhardtii
1.12.7.2 HydA2 isozyme Chlamydomonas reinhardtii
1.12.7.2 hydrogenase of the Fe-only type
-
 Chlamydomonas reinhardtii
1.12.7.2 [FeFe] hydrogenase
-
 Chlamydomonas reinhardtii
1.12.7.2 [FeFe] hydrogenase
-
 Tetradesmus obliquus
1.12.7.2 [FeFe] hydrogenase
-
 Clostridium pasteurianum
1.12.7.2 [FeFe] hydrogenase
-
 [Chlorella] fusca
1.12.7.2 [FeFe] hydrogenase
-
 Megasphaera elsdenii
1.12.7.2 [FeFe] hydrogenase
-
 Desulfovibrio desulfuricans
1.12.7.2 [FeFe] hydrogenase
-
 Clostridium acetobutylicum
1.12.7.2 [FeFe] hydrogenase
-
 Chlamydomonas moewusii
1.12.7.2 [FeFe] hydrogenase
-
 Chlorococcum submarinum

Cofactor

EC Number Cofactor Comment Organism Structure
1.12.7.2 Ferredoxin
-
 Chlamydomonas reinhardtii
1.12.7.2 Ferredoxin
-
 Tetradesmus obliquus
1.12.7.2 Ferredoxin
-
 Clostridium pasteurianum
1.12.7.2 Ferredoxin
-
 [Chlorella] fusca
1.12.7.2 Ferredoxin
-
 Megasphaera elsdenii
1.12.7.2 Ferredoxin
-
 Desulfovibrio desulfuricans
1.12.7.2 Ferredoxin
-
 Clostridium acetobutylicum
1.12.7.2 Ferredoxin
-
 Chlamydomonas moewusii
1.12.7.2 Ferredoxin
-
 Chlorococcum submarinum

Expression

EC Number Organism Comment Expression
1.12.7.2 Chlamydomonas reinhardtii activation and de novo synthesis of the protein is inhibited by cycloheximide but not chloramphenicol up