EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.22 | acetate | - |
Meretrix lusoria | |
1.5.1.22 | D-lactate | - |
Meretrix lusoria | |
1.5.1.22 | iminodiacetate | - |
Meretrix lusoria | |
1.5.1.22 | L-lactate | - |
Meretrix lusoria | |
1.5.1.22 | oxaloacetate | - |
Meretrix lusoria | |
1.5.1.22 | succinate | - |
Meretrix lusoria |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.22 | 1.39 | - |
pyruvate | pH 7.5, 25°C | Meretrix lusoria | |
1.5.1.22 | 34.2 | - |
glycine | pH 7.5, 25°C | Meretrix lusoria | |
1.5.1.22 | 39.2 | - |
L-Ala | pH 7.5, 25°C | Meretrix lusoria | |
1.5.1.22 | 45.9 | - |
L-2-aminobutyrate | pH 7.5, 25°C | Meretrix lusoria | |
1.5.1.22 | 163 | - |
L-Ser | pH 7.5, 25°C | Meretrix lusoria | |
1.5.1.22 | 196 | - |
beta-Ala | pH 7.5, 25°C | Meretrix lusoria | |
1.5.1.22 | 303 | - |
L-Thr | pH 7.5, 25°C | Meretrix lusoria |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.22 | Ca2+ | 10% of enzymatic activity is inhibited at 10 mM | Meretrix lusoria | |
1.5.1.22 | Cu2+ | 50% of enzymatic activity is inhibited at 1 mM | Meretrix lusoria | |
1.5.1.22 | Fe3+ | inhibits enzyme at 0.2 mM | Meretrix lusoria | |
1.5.1.22 | Mg2+ | 20% of enzymatic activity is inhibited at 10 mM | Meretrix lusoria | |
1.5.1.22 | Mn2+ | 13% of enzymatic activity is inhibited at 1 mM | Meretrix lusoria | |
1.5.1.22 | Zn2+ | inhibits enzyme at 0.6 mM | Meretrix lusoria |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.1.22 | 46000 | - |
SDS-PAGE | Meretrix lusoria |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.22 | Meretrix lusoria | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.22 | purified over 470fold using ammonium sulfate fractionation, sephacryl S-100 chromatography, hydroxyapatite adsorption, DEAE-sepharose chromatography, Blue-sepharose chromatography | Meretrix lusoria |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.5.1.22 | muscle | - |
Meretrix lusoria | - |
EC Number | Storage Stability | Organism |
---|---|---|
1.5.1.22 | 4°C, glycerol, 2 weeks, 100% activity | Meretrix lusoria |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.22 | beta-Ala + pyruvate + NADH | - |
Meretrix lusoria | ? | - |
? | |
1.5.1.22 | glycine + pyruvate + NADH | Gly and Ala are preferred substrates | Meretrix lusoria | D-strombine + NAD+ + H2O | - |
? | |
1.5.1.22 | L-2-Aminobutyrate + pyruvate + NADH | - |
Meretrix lusoria | ? | - |
? | |
1.5.1.22 | L-Ala + pyruvate + NADH | Gly and Ala are preferred substrates | Meretrix lusoria | ? | - |
? | |
1.5.1.22 | L-Ser + pyruvate + NADH | - |
Meretrix lusoria | ? | - |
? | |
1.5.1.22 | L-Thr + pyruvate + NADH | - |
Meretrix lusoria | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.1.22 | monomer | gel filtration, SDS-PAGE analysis, 1 * 46000 | Meretrix lusoria |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.1.22 | SDH | - |
Meretrix lusoria |
1.5.1.22 | strombine dehydrogenase | - |
Meretrix lusoria |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.1.22 | 25 | - |
assay at | Meretrix lusoria |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.1.22 | 45 | 46 | temperature optimum | Meretrix lusoria |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.1.22 | 56 | - |
enzyme is heat labile, pretreatment at 56°C for 20 sec only retains 20% of its original activity | Meretrix lusoria |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.22 | 7.5 | - |
assay at | Meretrix lusoria |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.22 | 7.4 | 7.6 | pH optimum | Meretrix lusoria |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.22 | 0.63 | - |
iminodiacetate | pH 7.5, 25°C | Meretrix lusoria | |
1.5.1.22 | 1.35 | - |
iminodiacetate | pH 6.5, 25°C | Meretrix lusoria | |
1.5.1.22 | 3.69 | - |
acetate | pH 7.5, 25°C | Meretrix lusoria | |
1.5.1.22 | 3.98 | - |
succinate | pH 6.5, 25°C | Meretrix lusoria | |
1.5.1.22 | 5.57 | - |
acetate | pH 6.5, 25°C | Meretrix lusoria | |
1.5.1.22 | 6.93 | - |
oxaloacetate | pH 7.5, 25°C | Meretrix lusoria | |
1.5.1.22 | 14.3 | - |
oxaloacetate | pH 6.5, 25°C | Meretrix lusoria | |
1.5.1.22 | 15.4 | - |
succinate | pH 7.5, 25°C | Meretrix lusoria | |
1.5.1.22 | 16.4 | - |
D-lactate | pH 6.5, 25°C | Meretrix lusoria | |
1.5.1.22 | 17 | - |
D-lactate | pH 7.5, 25°C | Meretrix lusoria | |
1.5.1.22 | 21.2 | - |
L-lactate | pH 6.5, 25°C | Meretrix lusoria | |
1.5.1.22 | 22.9 | - |
L-lactate | pH 7.5, 25°C | Meretrix lusoria |