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Literature summary extracted from

  • Sutton, D.L.; Loo, G.H.; Menz, R.I.; Schuller, K.A.
    Cloning and functional characterization of a typical 2-Cys peroxiredoxin from southern bluefin tuna (Thunnus maccoyii) (2010), Comp. Biochem. Physiol. B, 156, 97-106.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.24 Prx 2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, functional expression in Escherichia coli Thunnus maccoyii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.24 additional information
-
 additional information SBT Prx displays Michaelis-Menten kinetics with Trx but sigmoidal kinetics with H2O2 and CuOOH Thunnus maccoyii
1.11.1.24 0.012
-
 thioredoxin pH 7.5, 25°C, recombinant enzyme Thunnus maccoyii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.24 21800
-
 x * 21800, SDS-PAGE and sequence calculation Thunnus maccoyii
1.11.1.24 250000
-
 recombinant enzyme, peak 1, gel filtration Thunnus maccoyii
1.11.1.24 400000
-
 recombinant enzyme, peak 2, gel filtration Thunnus maccoyii

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.24 Thunnus maccoyii A9QKS0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.24 recombinant Prx 2 from Escherichia coli by nickel affinity chromatography and gel filtration Thunnus maccoyii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.24 2 thioredoxin + cumene hydroperoxide
-
 Thunnus maccoyii thioredoxin disulfide + H2O + 2-phenylpropan-2-ol
-
 ?
1.11.1.24 2 thioredoxin + H2O2
-
 Thunnus maccoyii thioredoxin disulfide + 2 H2O
-
 ?
1.11.1.24 2 thioredoxin + t-butyl hydroperoxide
-
 Thunnus maccoyii thioredoxin disulfide + H2O + t-butanol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.24 ? x * 21800, SDS-PAGE and sequence calculation Thunnus maccoyii
1.11.1.24 More the native SBT Prx enzyme exists as a mixture of dimers, tetramers, decamers and a higher order aggregate Thunnus maccoyii

Synonyms

EC Number Synonyms Comment Organism
1.11.1.24 Prx 2
-
 Thunnus maccoyii
1.11.1.24 typical 2-Cys peroxiredoxin
-
 Thunnus maccoyii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.24 25
-
 assay at Thunnus maccoyii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.24 6 7
-
 Thunnus maccoyii

General Information

EC Number General Information Comment Organism
1.11.1.24 additional information the fish Prx 2 contains the GGLG motif associated with the sensitivity of eukaryotic typical 2-Cys Prx proteins to overoxidation and consequent inactivation by H2O2 Thunnus maccoyii
1.11.1.24 physiological function peroxiredoxins are cysteine-dependent peroxidases that function as antioxidant enzymes and also in H2O2-mediated cell signaling Thunnus maccoyii