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Literature summary extracted from

  • Karki, S.; Yoo, H.G.; Kwon, S.Y.; Suh, J.W.; Kwon, H.J.
    Cloning and in vitro characterization of dTDP-6-deoxy-L-talose biosynthetic genes from Kitasatospora kifunensis featuring the dTDP-6-deoxy-L-lyxo-4-hexulose reductase that synthesizes dTDP-6-deoxy-L-talose (2010), Carbohydr. Res., 345, 1958-1962.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.344 expressed in Escherichia coli BL21 (DE3) cells Kitasatospora kifunensis
1.1.1.344 expression in Escherichia coli Kitasatospora kifunensis

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.344 Kitasatospora kifunensis
-
-
-
1.1.1.344 Kitasatospora kifunensis E5F146
-
-
1.1.1.344 Kitasatospora kifunensis MJM341
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.344 Ni-NTA agarose column chromatography Kitasatospora kifunensis
1.1.1.344 recombinant protein Kitasatospora kifunensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.344 dTDP-6-deoxy-beta-L-lyxo-4-hexulose + NADH + H+
-
 Kitasatospora kifunensis dTDP-6-deoxy-beta-L-talose + NAD+
-
 ?
1.1.1.344 dTDP-6-deoxy-beta-L-lyxo-4-hexulose + NADH + H+
-
 Kitasatospora kifunensis MJM341 dTDP-6-deoxy-beta-L-talose + NAD+
-
 ?
1.1.1.344 dTDP-6-deoxy-L-glucose-lyxo-4-hexulose + NADH + H+
-
 Kitasatospora kifunensis dTDP-6-deoxy-L-talose + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.344 dTDP-6-deoxy-L-lyxo-4-hexulose reductase
-
 Kitasatospora kifunensis
1.1.1.344 TAL
-
 Kitasatospora kifunensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.344 37
-
 assay at Kitasatospora kifunensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.344 7.5
-
 assay at Kitasatospora kifunensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.344 NADH
-
 Kitasatospora kifunensis