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Literature summary extracted from

  • Chen, H.Y.; Tan, X.L.; Lu, J.; Zhang, C.X.; Zhang, Y.; Yang, S.L.
    Characterization of ATPase activity of class II chaperonin from the hyperthermophilic archaeon Pyrococcus furiosus (2009), Biotechnol. Lett., 31, 1753-1758.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.6.1.7 ADP strongly competitively inhibits PfCPN ATPase activity. Addition of an equimolar amount of ADP results in a 48% inhibition of ATPase activity. When ADP concentration is four times that of ATP, the PfCPN ATPase activity decreases to 10% of that without ADP. Inhibition of PfCPN ATPase decreases its chaperonin activity in protecting lysozyme from heat-induced inactivation Pyrococcus furiosus
5.6.1.7 AMP when AMP concentration is 8 times that of ATP, AMP inhibits the ATPase activity to 48% Pyrococcus furiosus
5.6.1.7 Na+ ATPase activity is a little lower in the presence of 0.3 M Na+ plus 0.3 M K+, than in the presence of 0.3 M K+ alone Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.6.1.7 0.74
-
 ATP pH 7.5, 85°C Pyrococcus furiosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.6.1.7 Co2+ relatively low ATPase activity in presence of Co2+ or Mn2+ Pyrococcus furiosus
5.6.1.7 K+ strong ATPase activity in presence of K+, optimal concentration: 2 M Pyrococcus furiosus
5.6.1.7 Mg2+ ATPase activity depends on K+ and Mg2+, optimal concentration: 5-7.5 mM Mg2+ Pyrococcus furiosus
5.6.1.7 Mn2+ relatively low ATPase activity in presence of Co2+ or Mn2+ Pyrococcus furiosus
5.6.1.7 additional information in the presence of Cd2+, Ba2+, Ca2+ or Sr2+, little ATPase activity occurrs. Little activity is found in the presence of Na+ or Li+ Pyrococcus furiosus
5.6.1.7 NH4+ strong ATPase activity in presence of NH4+ Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
5.6.1.7 Pyrococcus furiosus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.1.7 ATP + H2O + a folded polypeptide chaperonin activity in protecting lysozyme from heat-induced inactivation Pyrococcus furiosus ADP + phosphate + an unfolded polypeptide
-
 ?
5.6.1.7 additional information PfCPN has almost no ADPase activity Pyrococcus furiosus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.6.1.7 PfCPN
-
 Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6.1.7 7.5
-
-
 Pyrococcus furiosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.6.1.7 7 9 pH 6.5: about 30% of maximal activity, pH 7.0: about 90% of maximal activity, pH 9.0: about 60% of maximal activity Pyrococcus furiosus