EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.3 | beta-mercaptoethanol | 161.09% activity at 1 mM | Klebsiella pneumoniae | |
1.2.1.3 | dithiothreitol | 147.48% activity at 1 mM | Klebsiella pneumoniae | |
1.2.1.3 | Semicarbazide hydrochloride | 118.98% activity at 1 mM | Klebsiella pneumoniae | |
1.2.1.99 | 2-mercaptoethanol | 1 mM, activity increases by 61% | Klebsiella pneumoniae | |
1.2.1.99 | dithiothreitol | 1 mM, activity increases by 47.5% | Klebsiella pneumoniae | |
1.2.1.99 | Semicarbazide hydrochloride | 1 mM, activity increases by 19% | Klebsiella pneumoniae |
EC Number | Application | Comment | Organism |
---|---|---|---|
1.2.1.99 | synthesis | PuuC is an active enzyme that can be utilized effectively for several purposes, including biological production of 3-hydroxypropionate | Klebsiella pneumoniae |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.1.3 | expressed in Escherichia coli BL21(DE3) cells (strain SH-KAD2) | Klebsiella pneumoniae |
1.2.1.99 | expressed in Escherichia coli | Klebsiella pneumoniae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.3 | Ag+ | complete inhibition at 1 mM | Klebsiella pneumoniae | |
1.2.1.3 | Co2+ | 89.01% residual activity at 1 mM | Klebsiella pneumoniae | |
1.2.1.3 | Cu2+ | complete inhibition at 1 mM | Klebsiella pneumoniae | |
1.2.1.3 | Fe2+ | 26.34% residual activity at 1 mM | Klebsiella pneumoniae | |
1.2.1.3 | Hg2+ | complete inhibition at 1 mM | Klebsiella pneumoniae | |
1.2.1.3 | Li+ | 90.64% residual activity at 1 mM | Klebsiella pneumoniae | |
1.2.1.3 | Mg2+ | 93.64% residual activity at 1 mM | Klebsiella pneumoniae | |
1.2.1.3 | Na+ | 89.17% residual activity at 1 mM | Klebsiella pneumoniae | |
1.2.1.3 | NH3 | 92.06% residual activity at 1 mM | Klebsiella pneumoniae | |
1.2.1.3 | Ni2+ | 89.54% residual activity at 1 mM | Klebsiella pneumoniae | |
1.2.1.3 | Zn2+ | 35.91% residual activity at 1 mM | Klebsiella pneumoniae | |
1.2.1.99 | Ag+ | 1 mM, complete inhibition | Klebsiella pneumoniae | |
1.2.1.99 | Co2+ | 1 mM, 11% inhibition | Klebsiella pneumoniae | |
1.2.1.99 | Cu2+ | 1 mM, complete inhibition | Klebsiella pneumoniae | |
1.2.1.99 | EDTA | 1 mM, 8.3% inhibition | Klebsiella pneumoniae | |
1.2.1.99 | Fe2+ | 1 mM, 73.6% inhibition | Klebsiella pneumoniae | |
1.2.1.99 | Hg2+ | 1 mM, complete inhibition | Klebsiella pneumoniae | |
1.2.1.99 | Li+ | 1 mM, 9.4% inhibition | Klebsiella pneumoniae | |
1.2.1.99 | Mg2+ | 1 mM, 6.3% inhibition | Klebsiella pneumoniae | |
1.2.1.99 | NaCl | mM, 11% inhibition | Klebsiella pneumoniae | |
1.2.1.99 | NH4Cl | 8% inhibition | Klebsiella pneumoniae | |
1.2.1.99 | Ni2+ | 1 mM, 10.5% inhibition | Klebsiella pneumoniae | |
1.2.1.99 | Zn2+ | 1 mM, 64% inhibition | Klebsiella pneumoniae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.3 | 0.09 | - |
NAD+ | recombinant enzyme, in 50 mM potassium phosphate buffer, at pH 8.0 and 45°C | Klebsiella pneumoniae | |
1.2.1.3 | 0.14 | - |
Valeraldehyde | recombinant enzyme, in 50 mM potassium phosphate buffer, at pH 8.0 and 45°C | Klebsiella pneumoniae | |
1.2.1.3 | 0.21 | - |
Isovaleraldehyde | recombinant enzyme, in 50 mM potassium phosphate buffer, at pH 8.0 and 45°C | Klebsiella pneumoniae | |
1.2.1.3 | 0.26 | - |
Butyraldehyde | recombinant enzyme, in 50 mM potassium phosphate buffer, at pH 8.0 and 45°C | Klebsiella pneumoniae | |
1.2.1.3 | 0.48 | - |
3-hydroxypropionaldehyde | recombinant enzyme, in 50 mM potassium phosphate buffer, at pH 8.0 and 45°C | Klebsiella pneumoniae | |
1.2.1.99 | 0.14 | - |
Valeraldehyde | pH 8.0, 45°C | Klebsiella pneumoniae | |
1.2.1.99 | 0.21 | - |
Isovaleraldehyde | pH 8.0, 45°C | Klebsiella pneumoniae | |
1.2.1.99 | 0.26 | - |
Butyraldehyde | pH 8.0, 45°C | Klebsiella pneumoniae | |
1.2.1.99 | 0.48 | - |
3-hydroxypropionaldehyde | pH 8.0, 45°C | Klebsiella pneumoniae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.1.3 | 53000 | - |
recombinant enzyme, SDS-PAGE | Klebsiella pneumoniae |
1.2.1.99 | 53800 | - |
x * 53800, SDS-PAGE | Klebsiella pneumoniae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.99 | 3-hydroxypropionaldehyde + NAD+ + H2O | Klebsiella pneumoniae | the enzyme might play a role in putresine and propionate metabolism | 3-hydroxypropanoate + NADH + 2 H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.3 | Klebsiella pneumoniae | - |
- |
- |
1.2.1.3 | Klebsiella pneumoniae DSM 2026 | - |
- |
- |
1.2.1.99 | Klebsiella pneumoniae | - |
- |
- |
1.2.1.99 | Klebsiella pneumoniae DSM 2026 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.1.3 | Ni-NTA column chromatography | Klebsiella pneumoniae |
1.2.1.99 | - |
Klebsiella pneumoniae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.2.1.3 | 3.13 | - |
crude extract, using 3-hydroxypropionaldehyde as substrate, in 50 mM, potassium phosphate buffer, at pH 8.0 and 45°C | Klebsiella pneumoniae |
1.2.1.3 | 27.73 | - |
after 8.86fold purification, using 3-hydroxypropionaldehyde as substrate, in 50 mM, potassium phosphate buffer, at pH 8.0 and 45°C | Klebsiella pneumoniae |
1.2.1.99 | 22.25 | - |
Vmax: 3-hydroxypropionaldehyde, pH 8.0, 45°C | Klebsiella pneumoniae |
1.2.1.99 | 28.61 | - |
Vmax: isovaleraldehyde, pH 8.0, 45°C | Klebsiella pneumoniae |
1.2.1.99 | 33.26 | - |
Vmax: valeraldehyde, pH 8.0, 45°C | Klebsiella pneumoniae |
1.2.1.99 | 34.08 | - |
Vmax: butyraldehyde, pH 8.0, 45°C | Klebsiella pneumoniae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.3 | 2-furaldehyde + NAD+ + H2O | the activity on 2-furaldehyde is 22% of that on 3-hydroxypropionaldehyde | Klebsiella pneumoniae | 2-furanoic acid + NADH + H+ | - |
? | |
1.2.1.3 | 3-hydroxypropionaldehyde + NAD+ + H2O | the activity on 3-hydroxypropionaldehyde is about 70% of that on valeraldehyde | Klebsiella pneumoniae | 3-hydroxypropanoate + NADH + 2 H+ | - |
? | |
1.2.1.3 | acetaldehyde + NAD+ + H2O | the activity on acetaldehyde is about 50% of that on valeraldehyde | Klebsiella pneumoniae | acetate + NADH + H+ | - |
? | |
1.2.1.3 | acetaldehyde + NAD+ + H2O | the activity on acetaldehyde is about 50% of that on valeraldehyde | Klebsiella pneumoniae DSM 2026 | acetate + NADH + H+ | - |
? | |
1.2.1.3 | benzaldehyde + NAD+ + H2O | the activity on benzaldehyde is 35% of that on 3-hydroxypropionaldehyde | Klebsiella pneumoniae | benzoate + NADH + H+ | - |
? | |
1.2.1.3 | benzaldehyde + NAD+ + H2O | the activity on benzaldehyde is 35% of that on 3-hydroxypropionaldehyde | Klebsiella pneumoniae DSM 2026 | benzoate + NADH + H+ | - |
? | |
1.2.1.3 | butyraldehyde + NAD+ + H2O | the activity on butyraldehyde is about 95% of that on valeraldehyde | Klebsiella pneumoniae | butanoate + NADH + H+ | - |
? | |
1.2.1.3 | isovaleraldehyde + NAD+ + H2O | the activity on isovaleraldehyde is about 80% of that on valeraldehyde | Klebsiella pneumoniae | isovalerate + NADH + H+ | - |
? | |
1.2.1.3 | additional information | no appreciable activity is observed on formaldehyde | Klebsiella pneumoniae | ? | - |
? | |
1.2.1.3 | additional information | no appreciable activity is observed on formaldehyde | Klebsiella pneumoniae DSM 2026 | ? | - |
? | |
1.2.1.3 | propionaldehyde + NAD+ + H2O | the activity on propionaldehyde is about 55% of that on valeraldehyde | Klebsiella pneumoniae | propionate + NADH + H+ | - |
? | |
1.2.1.3 | propionaldehyde + NAD+ + H2O | the activity on propionaldehyde is about 55% of that on valeraldehyde | Klebsiella pneumoniae DSM 2026 | propionate + NADH + H+ | - |
? | |
1.2.1.3 | valeraldehyde + NAD+ + H2O | most preferred substrate, 100% activity | Klebsiella pneumoniae | valerate + NADH + H+ | - |
? | |
1.2.1.3 | valeraldehyde + NAD+ + H2O | most preferred substrate, 100% activity | Klebsiella pneumoniae DSM 2026 | valerate + NADH + H+ | - |
? | |
1.2.1.99 | 2-furaldehyde + NAD+ + H2O | 22% of the activity with 3-hydroxypropionaldehyde | Klebsiella pneumoniae | furan-2-carboxylate + NADH + 2 H+ | - |
? | |
1.2.1.99 | 3-hydroxypropionaldehyde + NAD+ + H2O | - |
Klebsiella pneumoniae | 3-hydroxypropanoate + NADH + 2 H+ | - |
? | |
1.2.1.99 | 3-hydroxypropionaldehyde + NAD+ + H2O | the enzyme might play a role in putresine and propionate metabolism | Klebsiella pneumoniae | 3-hydroxypropanoate + NADH + 2 H+ | - |
? | |
1.2.1.99 | acetaldehyde + NAD+ + H2O | - |
Klebsiella pneumoniae | acetate + NADH + H+ | - |
? | |
1.2.1.99 | benzaldehyde + NAD+ + H2O | 35% of the activity with 3-hydroxypropionaldehyde | Klebsiella pneumoniae | benzoate + NADH + H+ | - |
? | |
1.2.1.99 | butyraldehyde + NAD+ + H2O | - |
Klebsiella pneumoniae | butyrate + NADH + 2 H+ | - |
? | |
1.2.1.99 | isovaleraldehyde + NAD+ + H2O | - |
Klebsiella pneumoniae | 3-methylbutanoate + NADH + 2 H+ | - |
? | |
1.2.1.99 | additional information | no activity with formaldehyde | Klebsiella pneumoniae | ? | - |
? | |
1.2.1.99 | propionaldehyde + NAD+ + H2O | - |
Klebsiella pneumoniae | propionate + NADH + 2 H+ | - |
? | |
1.2.1.99 | valeraldehyde + NAD+ + H2O | most preferred substrate | Klebsiella pneumoniae | pentanoate + NADH + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.1.99 | ? | x * 53800, SDS-PAGE | Klebsiella pneumoniae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.1.3 | ALDH | - |
Klebsiella pneumoniae |
1.2.1.3 | NAD+-dependent aldehyde dehydrogenase | - |
Klebsiella pneumoniae |
1.2.1.3 | NAD+-specific ALDH | - |
Klebsiella pneumoniae |
1.2.1.3 | PuuC | - |
Klebsiella pneumoniae |
1.2.1.99 | PuuC | - |
Klebsiella pneumoniae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.1.3 | 45 | - |
- |
Klebsiella pneumoniae |
1.2.1.99 | 45 | - |
- |
Klebsiella pneumoniae |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.1.99 | 20 | 60 | 20°C: about 30% of maximal activity, 60°C: about 35% of maximal activity | Klebsiella pneumoniae |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.1.3 | 30 | 60 | the enzyme is stable between 30°C and 50°C, but becomes unstable at 60°C | Klebsiella pneumoniae |
1.2.1.99 | 60 | - |
becomes unstable at 60°C | Klebsiella pneumoniae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.99 | 19.81 | - |
3-hydroxypropionaldehyde | pH 8.0, 45°C | Klebsiella pneumoniae | |
1.2.1.99 | 25.47 | - |
Isovaleraldehyde | pH 8.0, 45°C | Klebsiella pneumoniae | |
1.2.1.99 | 29.61 | - |
Valeraldehyde | pH 8.0, 45°C | Klebsiella pneumoniae | |
1.2.1.99 | 30.34 | - |
Butyraldehyde | pH 8.0, 45°C | Klebsiella pneumoniae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.3 | 8 | - |
- |
Klebsiella pneumoniae |
1.2.1.99 | 8 | - |
- |
Klebsiella pneumoniae |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.3 | 7 | 9 | - |
Klebsiella pneumoniae |
1.2.1.99 | 7 | 9 | pH 7.0: about 50% of maximal activity, pH 9.0: about 80% of maximal activity | Klebsiella pneumoniae |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.3 | 6 | - |
the enzyme activity is completely abolished at pH 6.0 | Klebsiella pneumoniae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.3 | NAD+ | NAD+ is the preferred coenzyme over NADP+ | Klebsiella pneumoniae | |
1.2.1.99 | NAD+ | preferred coenzyme over NADP+ | Klebsiella pneumoniae |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.3 | 41.44 | - |
3-hydroxypropionaldehyde | recombinant enzyme, in 50 mM potassium phosphate buffer, at pH 8.0 and 45°C | Klebsiella pneumoniae | |
1.2.1.3 | 117.2 | - |
Butyraldehyde | recombinant enzyme, in 50 mM potassium phosphate buffer, at pH 8.0 and 45°C | Klebsiella pneumoniae | |
1.2.1.3 | 121.9 | - |
Isovaleraldehyde | recombinant enzyme, in 50 mM potassium phosphate buffer, at pH 8.0 and 45°C | Klebsiella pneumoniae | |
1.2.1.3 | 207.1 | - |
Valeraldehyde | recombinant enzyme, in 50 mM potassium phosphate buffer, at pH 8.0 and 45°C | Klebsiella pneumoniae | |
1.2.1.3 | 254.7 | - |
NAD+ | recombinant enzyme, in 50 mM potassium phosphate buffer, at pH 8.0 and 45°C | Klebsiella pneumoniae | |
1.2.1.99 | 41.44 | - |
3-hydroxypropionaldehyde | pH 8.0, 45°C | Klebsiella pneumoniae | |
1.2.1.99 | 117.2 | - |
Butyraldehyde | pH 8.0, 45°C | Klebsiella pneumoniae | |
1.2.1.99 | 121.9 | - |
Isovaleraldehyde | pH 8.0, 45°C | Klebsiella pneumoniae | |
1.2.1.99 | 207.1 | - |
Valeraldehyde | pH 8.0, 45°C | Klebsiella pneumoniae |