EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.23 | EDTA | - |
Mycobacterium tuberculosis | |
3.5.1.23 | EGTA | - |
Mycobacterium tuberculosis | |
3.5.1.23 | sodium taurodeoxycholate | activates the recombinant enzyme about 2fold, optimally at 1% | Mycobacterium tuberculosis | |
3.5.1.23 | Triton X-100 | activates the recombinant enzyme about 2fold, optimally at 0.05% | Mycobacterium tuberculosis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.1.23 | expression of His6-tagged CDase in Escherichia coli strain BL21(DE3) | Mycobacterium tuberculosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.23 | Ca2+ | - |
Mycobacterium tuberculosis | |
3.5.1.23 | Co2+ | - |
Mycobacterium tuberculosis | |
3.5.1.23 | Cu2+ | - |
Mycobacterium tuberculosis | |
3.5.1.23 | Mg2+ | - |
Mycobacterium tuberculosis | |
3.5.1.23 | Mn2+ | - |
Mycobacterium tuberculosis | |
3.5.1.23 | Ni2+ | - |
Mycobacterium tuberculosis | |
3.5.1.23 | Zn2+ | - |
Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.23 | additional information | - |
additional information | Michaelis-Menten kinetics of hydrolysis, overview | Mycobacterium tuberculosis | |
3.5.1.23 | 0.0987 | - |
N-dodecanoyl-7-nitrobenz-2-oxa-1,3,4-diazole-D-erythro-sphingosine | pH 8.0, 37°C | Mycobacterium tuberculosis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.1.23 | 71000 | - |
x * 71000, recombinant enzyme, SDS-PAGE | Mycobacterium tuberculosis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.23 | ceramide + H2O | Mycobacterium tuberculosis | - |
sphingosine + fatty acid | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.23 | Mycobacterium tuberculosis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.23 | recombinant His-tagged CDase from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.23 | ceramide + H2O | - |
Mycobacterium tuberculosis | sphingosine + fatty acid | - |
r | |
3.5.1.23 | ceramide + H2O | the recombinant enzyme shows a broad subsrate specificity with ceramides, it shows good activity with ceramides containing C6-C24 fatty acids, and preference of ceramides with monounsaturated fatty acids than saturated fatty acids, overview | Mycobacterium tuberculosis | sphingosine + fatty acid | - |
r | |
3.5.1.23 | N-dodecanoyl-7-nitrobenz-2-oxa-1,3,4-diazole-D-erythro-dihydrosphingosine + H2O | - |
Mycobacterium tuberculosis | 12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]dodecanoate + D-erythro-dihydrosphingosine | - |
r | |
3.5.1.23 | N-dodecanoyl-7-nitrobenz-2-oxa-1,3,4-diazole-D-erythro-phytosphingosine + H2O | - |
Mycobacterium tuberculosis | 12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]dodecanate + D-erythro-phytosphingosine | - |
r | |
3.5.1.23 | N-dodecanoyl-7-nitrobenz-2-oxa-1,3,4-diazole-D-erythro-sphingosine + H2O | i.e. C12-NBD-Cer | Mycobacterium tuberculosis | 12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]dodecanate + D-erythro-sphingosine | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.1.23 | ? | x * 71000, recombinant enzyme, SDS-PAGE | Mycobacterium tuberculosis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.1.23 | CDase | - |
Mycobacterium tuberculosis |
3.5.1.23 | neutral ceramidase | - |
Mycobacterium tuberculosis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.23 | 37 | - |
assay at | Mycobacterium tuberculosis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.23 | 8 | 9 | recombinant enzyme, forward reaction | Mycobacterium tuberculosis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.23 | 4 | 10 | activity range, recombinant enzyme, forward reaction, profile overview | Mycobacterium tuberculosis |