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Literature summary extracted from

  • Okino, N.; Ikeda, R.; Ito, M.
    Expression, purification, and characterization of a recombinant neutral ceramidase from Mycobacterium tuberculosis (2010), Biosci. Biotechnol. Biochem., 74, 316-321.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.23 EDTA
-
Mycobacterium tuberculosis
3.5.1.23 EGTA
-
Mycobacterium tuberculosis
3.5.1.23 sodium taurodeoxycholate activates the recombinant enzyme about 2fold, optimally at 1% Mycobacterium tuberculosis
3.5.1.23 Triton X-100 activates the recombinant enzyme about 2fold, optimally at 0.05% Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.23 expression of His6-tagged CDase in Escherichia coli strain BL21(DE3) Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.23 Ca2+
-
Mycobacterium tuberculosis
3.5.1.23 Co2+
-
Mycobacterium tuberculosis
3.5.1.23 Cu2+
-
Mycobacterium tuberculosis
3.5.1.23 Mg2+
-
Mycobacterium tuberculosis
3.5.1.23 Mn2+
-
Mycobacterium tuberculosis
3.5.1.23 Ni2+
-
Mycobacterium tuberculosis
3.5.1.23 Zn2+
-
Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.23 additional information
-
additional information Michaelis-Menten kinetics of hydrolysis, overview Mycobacterium tuberculosis
3.5.1.23 0.0987
-
N-dodecanoyl-7-nitrobenz-2-oxa-1,3,4-diazole-D-erythro-sphingosine pH 8.0, 37°C Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.23 71000
-
x * 71000, recombinant enzyme, SDS-PAGE Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.23 ceramide + H2O Mycobacterium tuberculosis
-
sphingosine + fatty acid
-
r

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.23 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.23 recombinant His-tagged CDase from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.23 ceramide + H2O
-
Mycobacterium tuberculosis sphingosine + fatty acid
-
r
3.5.1.23 ceramide + H2O the recombinant enzyme shows a broad subsrate specificity with ceramides, it shows good activity with ceramides containing C6-C24 fatty acids, and preference of ceramides with monounsaturated fatty acids than saturated fatty acids, overview Mycobacterium tuberculosis sphingosine + fatty acid
-
r
3.5.1.23 N-dodecanoyl-7-nitrobenz-2-oxa-1,3,4-diazole-D-erythro-dihydrosphingosine + H2O
-
Mycobacterium tuberculosis 12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]dodecanoate + D-erythro-dihydrosphingosine
-
r
3.5.1.23 N-dodecanoyl-7-nitrobenz-2-oxa-1,3,4-diazole-D-erythro-phytosphingosine + H2O
-
Mycobacterium tuberculosis 12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]dodecanate + D-erythro-phytosphingosine
-
r
3.5.1.23 N-dodecanoyl-7-nitrobenz-2-oxa-1,3,4-diazole-D-erythro-sphingosine + H2O i.e. C12-NBD-Cer Mycobacterium tuberculosis 12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]dodecanate + D-erythro-sphingosine
-
r

Subunits

EC Number Subunits Comment Organism
3.5.1.23 ? x * 71000, recombinant enzyme, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
3.5.1.23 CDase
-
Mycobacterium tuberculosis
3.5.1.23 neutral ceramidase
-
Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.23 37
-
assay at Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.23 8 9 recombinant enzyme, forward reaction Mycobacterium tuberculosis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.23 4 10 activity range, recombinant enzyme, forward reaction, profile overview Mycobacterium tuberculosis